- Epidemiological relationships of Campylobacter jejuni strains isolated from humans and chickens in South Korea
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Jae-Young Oh , Yong-Kuk Kwon , Bai Wei , Hyung-Kwan Jang , Suk-Kyung Lim , Cheon-Hyeon Kim , Suk-Chan Jung , Min-Su Kang
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J. Microbiol. 2017;55(1):13-20. Published online December 30, 2016
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DOI: https://doi.org/10.1007/s12275-017-6308-8
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Abstract
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Thirty-nine human isolates of Campylobacter jejuni obtained
from a national university hospital during 2007–2010 and
38 chicken isolates of C. jejuni were collected from poultry
farms during 2009–2010 in South Korea were used in this
study. Campylobacter genomic species and virulence-associated
genes were identified by PCR. Pulsed-field gel electrophoresis
(PFGE) and multilocus sequence typing (MLST)
were performed to compare their genetic relationships. All
isolates were highly resistant to ciprofloxacin, nalidixic acid,
and tetracycline. Of all isolates tested, over 94% contained
seven virulence associated genes (flaA, cadF, racR, dnaJ, cdtA,
cdtB, and cdtC). All isolates were classified into 39 types by
PFGE clustering with 90% similarity. Some chicken isolates
were incorporated into some PFGE types of human isolates.
MLST analysis for the 39 human isolates and 38 chicken isolates
result ed in 14 and 23 sequence types (STs), respectively,
of which 10 STs were new. STs overlapped in both chicken
and human isolates included ST-21, ST-48, ST-50, ST-51,
and ST-354, of which ST-21 was the predominant ST in both
human and chicken isolates. Through combined analysis of
PFGE types and STs, three chicken isolates were clonally related
to the three human isolates associated with food poisoning
(VII-ST-48, XXII-ST-354, and XXVIII-ST-51). They
were derived from geographically same or distinct districts.
Remarkably, clonal spread of food poisoning pathogens between
animals and humans was confirmed by population
genetic analysis. Consequently, contamination of campylobacters
with quinolone resistance and potential virulence genes in poultry production and consumption may increase
the risk of infections in humans.
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- Epidemiological Investigation of eaeA-Positive Escherichia coli and Escherichia albertii Strains Isolated from Healthy Wild Birds
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Jae-Young Oh , Min-Su Kang , Hee-Tae Hwang , Byung-Ki An , Jun-Hun Kwon , Yong-Kuk Kwon
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J. Microbiol. 2011;49(5):747-752. Published online November 9, 2011
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DOI: https://doi.org/10.1007/s12275-011-1133-y
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Escherichia coli has commonly been associated with diarrheal illness in humans and animals. Recently, E. albertii has been reported to be a potential pathogen of humans and animals and to be carried by wild birds. In the present study, the prevalence and genetic characteristics of intimin-producing E. coli and E. albertii strains were evaluated in wild birds in Korea. Thirty one of 790 Enterobacteriaceae strains from healthy wild birds were positive for the intimin gene (eaeA) and twenty two of the 31 strains were identified as atypical enteropathogenic E. coli (aEPEC) that did not possess both EAF and bfpA genes. A total of nine lactose non-fermenting coliform bacterial strains were identified as E. albertii by PCR and sequence analysis of housekeeping genes. A total of 28 (90.3%) eaeA-positive strains were isolated from waterfowl. Fifteen aEPEC (68.2%) and two E. albertii (22.2%) strains had a β-intimin subtype and 14 aEPEC strains harboring β-intimin belonged to phylogenetic group B2. All eaeA-positive E. albertii and 3 aEPEC strains possessed the cytolethal distending toxin gene (cdtB). The eaeA-positive E. coli and E. albertii strains isolated from healthy wild birds need to be recognized as a potential pathogroup that may pose a potential threat to human and animal health. These findings indicate that eaeA-positive E. coli as well as E. albertii can be carried by wild birds, posing a potential threat to human and animal health.
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