Journal of Microbiology

Jae-Yong Cho

3 Articles

Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain: Hong-Il Kim , Jae-Young Nam , Jae-Yong Cho , Chang-Soo Lee , Young-Jin Park; J. Microbiol. 2013;51(6):877-880. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3236-0

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In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or downregulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation.

Citations

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Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum
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Microorganisms.2024; 12(8): 1681. CrossRef
Transcriptome analysis of l-leucine-producing Corynebacterium glutamicum under the addition of trimethylglycine
Jian Wang, Xuesong Wang, Qing Liang, Deheng Li, Dawei Li, Qunqun Guo
Amino Acids.2022; 54(2): 229. CrossRef
RETRACTED ARTICLE: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels
Xiuxia Liu, Sun Yang, Fen Wang, Xiaofeng Dai, Yankun Yang, Zhonghu Bai
Journal of Industrial Microbiology and Biotechnology.2017; 44(2): 181. CrossRef
Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l-Lysine Production in Corynebacterium glutamicum
Hong-Il Kim, Jong-Hyeon Kim, Young-Jin Park
International Journal of Molecular Sciences.2016; 17(3): 353. CrossRef

Next-Generation Sequencing-Based Genome-Wide Mutation Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain: Chang-Soo Lee , Jae-Young Nam , Eun-Suk Son , O-chul Kwon , Woorijarang Han , Jae-Yong Cho , Young-Jin Park; J. Microbiol. 2012;50(5):860-863. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2109-2

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Abstract PDF

In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acidproducing strain with minimal mutation.

Citations

Citations to this article as recorded by

Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum
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Microorganisms.2024; 12(8): 1681. CrossRef
Recent advances in the metabolic engineering and physiological opportunities for microbial synthesis of L-aspartic acid family amino acids: A review
Yusheng Wang, Yunlong Bai, Qi Zeng, Zeyuan Jiang, Yuzhe Liu, Xiyan Wang, Xiaoting Liu, Chunlei Liu, Weihong Min
International Journal of Biological Macromolecules.2023; 253: 126916. CrossRef
Obtaining a series of native gradient promoter-5′-UTR sequences in Corynebacterium glutamicum ATCC 13032
Ning Li, Weizhu Zeng, Sha Xu, Jingwen Zhou
Microbial Cell Factories.2020;[Epub] CrossRef
The BFK20 phage replication origin confers a phage-encoded resistance phenotype to the industrial strain Brevibacterium flavum
Gabriela Bukovska, Jana Ugorcakova, Nora Halgasova, Lucia Bocanova, Adela Tkacova
FEMS Microbiology Letters.2019;[Epub] CrossRef
L-lysine production improvement: a review of the state of the art and patent landscape focusing on strain development and fermentation technologies
Fernanda Karine do Carmo Félix, Luiz Alberto Junior Letti, Gilberto Vinícius de Melo Pereira, Pedro Gabriel Borges Bonfim, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Critical Reviews in Biotechnology.2019; 39(8): 1031. CrossRef
Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids
Junjie Yang, Sheng Yang
BMC Genomics.2017;[Epub] CrossRef
White biotechnology: State of the art strategies for the development of biocatalysts for biorefining
S. Heux, I. Meynial-Salles, M.J. O'Donohue, C. Dumon
Biotechnology Advances.2015; 33(8): 1653. CrossRef
Characterization of a Corynebacterium glutamicum dnaB mutant that shows temperature-sensitive growth and mini-cell formation
Makoto Uchida, Takashi Hirasawa, Masaaki Wachi
Archives of Microbiology.2014; 196(12): 871. CrossRef
Next-generation sequencing-based transcriptome analysis of l-lysine-producing Corynebacterium glutamicum ATCC 21300 strain
Hong-Il Kim, Jae-Young Nam, Jae-Yong Cho, Chang-Soo Lee, Young-Jin Park
Journal of Microbiology.2013; 51(6): 877. CrossRef

Recombinant Expression and Purification of Functional XorII, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae: Dong Kyu Hwang , Jae-Yong Cho , Young Kee Chae; J. Microbiol. 2007;45(2):175-178.; DOI: https://doi.org/2515 [pii]

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Abstract PDF: An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorII, was recombinantly produced in Escherichia coli using a T7 system. XorII was purified using a combination of ion exchange and immobilized metal affinity chromatography (IMAC). An optimized washing protocol was carried out on an IMAC in order to obtain a high purity product. The final amount of purified XorII was approximately 2.5 mg/L of LB medium. The purified recombinant XorII was functional and showed the same cleavage pattern as PvuI. The enzyme activity tested the highest at 25°C in 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, and 1 mM dithiothreitol at a pH of 7.9.

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