- Establishment and Characterization of the Epithelioma Papulosum Cyprini (EPC) Cell Line Persistently Infected with Infectious Pancreatic Necrosis Virus (IPNV), an Aquabirnavirus
-
Hyoung Jun Kim , Jae-Kwon Cho , Hyung-Kyu Hwang , Myung-Joo Oh , Toyohiko Nishizawa
-
J. Microbiol. 2012;50(5):821-826. Published online November 4, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2364-2
-
-
868
View
-
1
Download
-
11
Crossref
-
 Abstract
 PDF
-
Infectious pancreatic necrosis virus (IPNV), a type species of aquabirnaviruses in the family Birnaviridae, is an etiological agent of infectious pancreatic necrosis and has been isolated from epizootics of cultured salmonids. In the present study, an epithelioma papulosum cyprini (EPC) cell line persistently infected with IPNV (PI-EPC) was experimentally established by subculturing EPC cells surviving IPNV infection, and was characterized. PI-EPC cells were morphologically indistinguishable from EPC, but continued to grow and yield IPNV. PI-EPC cells showed no cytopathic effect due to IPNV inoculation, and susceptibility of PI-EPC cells against heterologous viruses was not different from that of EPC cells. Only one cell of 103.5 PI-EPC cells produced IPNV at approximately 100.5 50% tissue culture infectious dose (TCID50)/cell/day, which was approximately 1,000 times lower than that of normal EPC cells. PI-EPC cells that did not yield IPNV (N-PI-EPC) were screened. The IPNV genome was detected from both PI-EPC and N-PI-EPC cells, and the IPNV VP2 structural protein was detected from both cell lines, but no other IPNV proteins were observed by Western blot analysis with anti-IPNV serum. Thus, multiplication of IPNV in PI-EPC cells was regulated by some host cell factors, except interferon.
-
Citations
Citations to this article as recorded by  - Kinetic control of nervous necrosis virus by cell-derived antiviral substance and metabolic state in fish cell lines
Han Sol Lee, Toyohiko Nishizawa
Virology.2026; 615: 110767. CrossRef - Characterization of cell lines persistently infected with infectious pancreatic necrosis virus
Lise Chaumont, Mathilde Peruzzi, François Huetz, Claudine Raffy, Jérôme Le Hir, Jules Minke, Pierre Boudinot, Bertrand Collet
Journal of General Virology
.2026;[Epub] CrossRef - Potential involvement of persistently infected cells in protective immunity of grouper fish convalesced from nervous necrosis virus infection
Toyohiko Nishizawa
Virology.2026; 624: 111040. CrossRef - Fish cells persistently infected with nervous necrosis virus produce a small-molecule substance for reducing cellular metabolism and suppressing viral multiplication
Han Sol Lee, Toyohiko Nishizawa
Scientific Reports.2025;[Epub] CrossRef - RNA-seq analysis of a zebrafish caudal fin cell line in response to infection with spring viraemia of carp virus
Wenji Huang, Ning Ji, Xin Zhao, Jiahong Guo, Jianhua Feng, Kangyong Chen, Yaxin Wu, Junya Wang, Jun Zou
Aquaculture and Fisheries.2024; 9(2): 181. CrossRef - Development and characterization of megalocytivirus persistently-infected cell cultures for high yield of virus
Woo Ju Kwon, Min Ji Yoon, Ji Woong Jin, Kwang Il Kim, Young Chul Kim, Suhee Hong, Joon Bum Jeong, Hyun Do Jeong
Tissue and Cell.2020; 66: 101387. CrossRef - Characterization of Persistent Infection of Megalocytivirus in PMF Cell Line Derived from the Fin of Red Sea Bream Pagrus major
Min-Gyeong JEONG, Woo-Ju KWON, Joon-Gyu MIN, Hyun-Do JEONG
JOURNAL OF FISHRIES AND MARINE SCIENCES EDUCATION.2019; 31(6): 1639. CrossRef - INFECTIOUS PANCREATIC NECROSIS OF SALMONID FISH - DISTRIBUTION AND LABORATORY METHODS FOR DIAGNOSIS
E. Mileva
Trakia Journal of Sciences.2019; 17(4): 401. CrossRef - Infectious Hematopoietic Necrosis
Toyohiko Nishizawa, Mamoru Yoshimizu
Fish Pathology.2016; 52(1): 1. CrossRef - Multiple Passages of Grunt Fin Cells Persistently Infected with Red Seabream Iridovirus (RSIV) at 15ºC or 30ºC to Yield Uninfected Cells
So-Young Oh, Toyohiko Nishizawa
Journal of Aquatic Animal Health.2016; 28(4): 214. CrossRef - A Novel Disorder of Cultured Juvenile Greater Amberjack Seriola dumerili Characterized by Renomegaly and Splenomegaly
Shinpei Wada, Osamu Kurata, Hitoshi Hatakeyama, Azumi Yamashita, Shusaku Takagi, Toyohiko Nishizawa, Hiroshi Yokoyama
Fish Pathology.2014; 49(1): 7. CrossRef
|
MSK
