- Comparison of virulence between matt and mucoid colonies of Klebsiella pneumoniae coproducing NDM-1 and OXA-232 isolated from a single patient
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Haejeong Lee , Jin Yang Baek , So Yeon Kim , HyunJi Jo , KyeongJin Kang , Jae-Hoon Ko , Sun Young Cho , Doo Ryeon Chung , Kyong Ran Peck , Jae-Hoon Song , Kwan Soo Ko
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J. Microbiol. 2018;56(9):665-672. Published online August 23, 2018
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DOI: https://doi.org/10.1007/s12275-018-8130-3
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Nine Klebsiella pneumoniae isolates coproducing NDM-1
and OXA-232 carbapenemases were successively isolated
from a single patient. Although they were isolated simultaneously
and were isogenic, they presented different colony
phenotypes (matt and mucoid). All nine isolates were resistant
to most antibiotics except colistin and fosfomycin. In
addition, matt-type isolates were resistant to tigecycline. No
differences were detected in the cps cluster sequences, except
for the insertion of IS5 in the wzb gene of two matt-type isolates.
In vitro virulence assays based on production of capsular
polysaccharide, biofilm formation, and resistance to
human serum indicated that the mucoid-type isolates were
significantly more virulent than the matt-type. In addition,
mucoid-type isolates showed higher survival rates than the
matt-type ones in infection experiments in the fruit fly, suggesting
a higher virulence of K. pneumoniae isolates with a
mucoid phenotype. To our knowledge, this is the first report
of K. pneumoniae colonies with different phenotypes being
isolated from the same sample. In addition, we show that virulence
varies with colony phenotype. Dissemination of K.
pneumoniae isolates expressing both antibiotic resistance
and high virulence would constitute a great threat.
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Citations
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- Characteristics of the community-genotype sequence type 72 methicillin-resistant Staphylococcus aureus isolates that underlie their persistence in hospitals
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Eun-Jeong Joo , Ji-Young Choi , Doo Ryeon Chung , Jae-Hoon Song , Kwan Soo Ko
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J. Microbiol. 2016;54(6):445-450. Published online May 27, 2016
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DOI: https://doi.org/10.1007/s12275-016-6157-x
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Abstract
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Panton-Valentine leukocidin-negative methicillin-resistant
Staphylococcus aureus (MRSA) clone ST72, known as a major
community-associated MRSA in Korea, has emerged as an
important pathogen in hospitals. To understand bacterial
properties that underlie transformation of this clone into a
nosocomial pathogen, we compared characteristics of the
community-genotype ST72 MRSA isolates with those of ST5
and ST239 MRSA, which have been predominant nosocomial
MRSA clones in Korea. Several genes associated with
adhesion and virulence were absent or rarely found in ST72
isolates. Many ST72 isolates (70.1%) belonged to agr group I,
but the agr group of other ST72 isolates could not be determined.
As indicated by δ-hemolysin production, ST72 isolates
expressed fully functional agr, whereas agr dysfunction
was observed in ST5 and ST239 isolates. In the biofilm formation
assay, no upregulation of biofilm-forming activity
of ST72 MRSA was detected. However, ST72 isolates demonstrated
persistence under hypotonic and desiccating conditions
(survival rates 72.3% and 33.9%, respectively), which
was similar to characteristics of ST5 or ST239 isolates. ST72-
MRSA isolates showed low virulence, but properties of their
functional agr system could facilitate their spread in hospitals.
In conclusion, tolerance to stressful environments, e.g., hypotonic
and dry conditions, may also contribute to survival
of the community-associated MRSA clones in healthcare
facilities.
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