- Comparison of virulence between matt and mucoid colonies of Klebsiella pneumoniae coproducing NDM-1 and OXA-232 isolated from a single patient
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Haejeong Lee , Jin Yang Baek , So Yeon Kim , HyunJi Jo , KyeongJin Kang , Jae-Hoon Ko , Sun Young Cho , Doo Ryeon Chung , Kyong Ran Peck , Jae-Hoon Song , Kwan Soo Ko
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J. Microbiol. 2018;56(9):665-672. Published online August 23, 2018
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DOI: https://doi.org/10.1007/s12275-018-8130-3
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Nine Klebsiella pneumoniae isolates coproducing NDM-1
and OXA-232 carbapenemases were successively isolated
from a single patient. Although they were isolated simultaneously
and were isogenic, they presented different colony
phenotypes (matt and mucoid). All nine isolates were resistant
to most antibiotics except colistin and fosfomycin. In
addition, matt-type isolates were resistant to tigecycline. No
differences were detected in the cps cluster sequences, except
for the insertion of IS5 in the wzb gene of two matt-type isolates.
In vitro virulence assays based on production of capsular
polysaccharide, biofilm formation, and resistance to
human serum indicated that the mucoid-type isolates were
significantly more virulent than the matt-type. In addition,
mucoid-type isolates showed higher survival rates than the
matt-type ones in infection experiments in the fruit fly, suggesting
a higher virulence of K. pneumoniae isolates with a
mucoid phenotype. To our knowledge, this is the first report
of K. pneumoniae colonies with different phenotypes being
isolated from the same sample. In addition, we show that virulence
varies with colony phenotype. Dissemination of K.
pneumoniae isolates expressing both antibiotic resistance
and high virulence would constitute a great threat.
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