Journal of Microbiology

Jae-Hong Kim

3 Articles

Evolution of a major bovine mastitic genotype (rpoB sequence type 10-2) of Staphylococcus aureus in cows: Dae-Sung Ko , Danil Kim , Eun-Kyung Kim , Jae-Hong Kim , Hyuk-Joon Kwon; J. Microbiol. 2019;57(7):587-596. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8699-1

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Staphylococcus aureus is the major pathogen leading to bovine mastitis globally while livestock-associated methicillin resistant S. aureus (LA-MRSA) has become a potential threat to public health. MRSA from bovine mastitis is not common but a methicillin susceptible S. aureus (MSSA) genotype, rpoB sequence type (RST)10-2 (RST10-2), is prevalent in Korea. To date, many genomic sequences from S. aureus have been elucidated, but the complete genome sequences of RST10-2 MSSA from bovine mastitis has never been reported. In this study, we determined the complete genome sequence of two RST10-2 MSSA that differ from each other in staphylococcal protein A and molecular prophage types [PMB64-1 (t2489/ mPPT0) and PMB81-4 (t127/mPPT1-2-3)] and conducted a comparative genomics study. The genomic sequences of PMB64-1 and PMB81-4 were more homologous to the representative human RST10-2 strains (MSSA476, MW2 etc.) compared to other RSTs. Most of them shared five common pseudogenes, along with high amino acid identity of four variable virulence genes that were identified in this study. However, PMB64-1 and PMB81-4 acquired different strainspecific pseudogenes and mobile genetic elements than the human strains. The unique pseudogene profile and high identity of the virulence genes were verified in RST10-2 field strains from bovine mastitis. Thus, bovine mastitic RST10-2 MSSA may have an evolutionary relationship with the human RST10- 2 community-associated (CA) MSSA and CA-MRSA strains but may have adapted to cows.

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Antimicrobial resistance and molecular characteristics of bovine mastitis-associated methicillin-resistant Staphylococcus aureus : potential for cross-species transmission of ST59-MRSA
Wen Sun, Jiayi Liu, Shuangshuang Li, Xiaoman Zhu, Xiangyun Wu, Baojing Dou, Xiaomin Pang, Keke Tian, Peipei Wang, Haihong Hao, Yulian Wang, Philips O. Akinwole
Microbiology Spectrum.2025;[Epub] CrossRef
An rpoB Sequence Type Network as a Framework for the Evolutionary Investigation of Clostridium perfringens
Sun-Min Ahn, Seungeun Son, Yongwoo Son, So-Jeong Lim, Danil Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Microorganisms.2025; 13(12): 2768. CrossRef
Rapid Antibacterial Activity Assessment of Chimeric Lysins
Jin-Mi Park, Jun-Hyun Kim, Gun Kim, Hun-Ju Sim, Sun-Min Ahn, Kang-Seuk Choi, Hyuk-Joon Kwon
International Journal of Molecular Sciences.2024; 25(4): 2430. CrossRef
Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli
Eun-Jin Ha, Seung-Min Hong, Seung-Ji Kim, Sun-Min Ahn, Ho-Won Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(12): 1714. CrossRef
Genetic characterization of Staphylococcus aureus isolated from Norway rats in Boston, Massachusetts
Gracen R. Gerbig, Helen Piontkivska, Tara C. Smith, Ruairi White, Jean Mukherjee, Hayley Benson, Marieke Rosenbaum, Jessica H. Leibler
Veterinary Medicine and Science.2023; 9(1): 272. CrossRef
Rapid Screening and Comparison of Chimeric Lysins for Antibacterial Activity against Staphylococcus aureus Strains
Jin-Mi Park, Dae-Sung Ko, Hee-Soo Kim, Nam-Hyung Kim, Eun-Kyoung Kim, Young-Hye Roh, Danil Kim, Jae-Hong Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(4): 667. CrossRef
Comparative genomics of bovine mastitis-origin Staphylococcus aureus strains classified into prevalent human genotypes
Dae-Sung Ko, Nam-Hyung Kim, Eun-Kyung Kim, Eun-Jin Ha, Young-Hye Ro, Danil Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Research in Veterinary Science.2021; 139: 67. CrossRef

Molecular Serotyping of Salmonella enterica by Complete rpoB Gene Sequencing: Won-Jin Seong , Hyuk-Joon Kwon , Tae-Eun Kim , Deog-Yong Lee , Mi-Sun Park , Jae-Hong Kim; J. Microbiol. 2012;50(6):962-969. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2547-x

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Abstract PDF

Serotyping has been the gold standard for identifying Salmonella, but it requires large amounts of standard antisera. Multilocus sequence typing (MLST) has been applied to identify Salmonella serovars, but the recombination of 4–7 housekeeping genes and multiple analytic steps diminish its applicability. In the present study, we determined the complete sequences of the RNA polymerase beta subunit gene (rpoB) and 7 housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA) for 76 strains of 33 Salmonella enterica serovars and conducted phylogenetic analyses together with the corresponding gene sequences of 24 reference strains registered in the GenBank database. Based on the phylogenetic analyses, 100 strains from 40 serovars and 91 strains from 37 serovars were classified into 60 rpoB (RST) and 49 multilocus sequence types (ST), respectively. The nucleotide similarities were 98.8–100% and 96.9–100% for the complete rpoB gene and the seven concatenated housekeeping genes, respectively. The strains of 35 and 30 serovars formed serovar-specific branches or clusters in the rpoB and housekeeping gene phylogenetic trees, respectively. Therefore, complete rpoB gene sequencing and phylogenetic analysis may be a useful method for identifying Salmonella serovars that is a simpler, more cost-effective, and less time-consuming alternative or complementary method to MLST and conventional serotyping.

Citations

Citations to this article as recorded by

An rpoB Sequence Type Network as a Framework for the Evolutionary Investigation of Clostridium perfringens
Sun-Min Ahn, Seungeun Son, Yongwoo Son, So-Jeong Lim, Danil Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Microorganisms.2025; 13(12): 2768. CrossRef
Shared Extended-Spectrum β-Lactamase-Producing Salmonella Serovars between Agricultural and Aquatic Environments Revealed through invA Amplicon Sequencing
Cecilia Mahlatse Raseala, Mutshiene Deogratias Ekwanzala, Maggy Ndombo Benteke Momba
Microorganisms.2020; 8(12): 1898. CrossRef
Molecular evolution of Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum in the field
Nam-Hyung Kim, Eun-Jin Ha, Dae-Sung Ko, Chung-Young Lee, Jae-Hong Kim, Hyuk-Joon Kwon
Veterinary Microbiology.2019; 235: 63. CrossRef
Molecular prophage typing ofStaphylococcus aureusisolates from bovine mastitis
Dae-Sung Ko, Won-Jin Seong, Danil Kim, Eun-Kyung Kim, Nam-Hyung Kim, Chung-Young Lee, Jae-Hong Kim, Hyuk-Joon Kwon
Journal of Veterinary Science.2018; 19(6): 771. CrossRef
Quantifying the Survival of Multiple Salmonella enterica Serovars In Vivo via Massively Parallel Whole-Genome Sequencing To Predict Zoonotic Risk
Prerna Vohra, Marie Bugarel, Frances Turner, Guy H. Loneragan, Jayne C. Hope, John Hopkins, Mark P. Stevens, Harold L. Drake
Applied and Environmental Microbiology.2018;[Epub] CrossRef
Phage‐Based Biosensor and Optimization of Surface Blocking Agents to Detect Salmonella Typhimurium on Romaine Lettuce
Jacqueline D. Mack, Teshome Yehualaeshet, Mi‐Kyung Park, Berhanu Tameru, Temesgen Samuel, Bryan A. Chin
Journal of Food Safety.2017;[Epub] CrossRef
Genotyping of Salmonella with lineage-specific genes: correlation with serotyping
Qing-Hua Zou, Ren-Qing Li, Gui-Rong Liu, Shu-Lin Liu
International Journal of Infectious Diseases.2016; 49: 134. CrossRef
Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase
Jean Guard, Zaid Abdo, Sara Overstreet Byers, Patrick Kriebel, Michael J. Rothrock
Foodborne Pathogens and Disease.2016; 13(7): 350. CrossRef
Phylogenetics of family Enterobacteriaceae and proposal to reclassify Escherichia hermannii and Salmonella subterranea as Atlantibacter hermannii and Atlantibacter subterranea gen. nov., comb. nov.
Hiroyuki Hata, Tatsuya Natori, Takuya Mizuno, Izumi Kanazawa, Ibrahim Eldesouky, Masahiro Hayashi, Machiko Miyata, Hajime Fukunaga, Shoko Ohji, Akira Hosoyama, Eiji Aono, Atsushi Yamazoe, Keiko Tsuchikane, Nobuyuki Fujita, Takayuki Ezaki
Microbiology and Immunology.2016; 60(5): 303. CrossRef
rpoB gene sequencing for phylogenetic analysis of avian pathogenic Escherichia coli
Hyuk-Joon Kwon, Won-Jin Seong, Tae-Eun Kim, Yong-Jin Won, Jae-Hong Kim
Korean Journal of Veterinary Research.2015; 55(1): 31. CrossRef
Modified Method of Multilocus Sequence Typing (MLST) for Serotyping inSalmonellaSpecies
Young-Sun Yun, Su-Jin Chae, Hye-Young Na, Gyung Tae Chung, Cheon-Kwon Yoo, Deog-Yong Lee
Journal of Bacteriology and Virology.2015; 45(4): 314. CrossRef
Serotype-associated polymorphisms in a partial rpoB gene sequence of Salmonella enterica
Marcela Fresno, Marlen Barreto, Sindy Gutierrez, Catherine Dougnac, Pedro Abalos, Patricio Retamal
Canadian Journal of Microbiology.2014; 60(3): 177. CrossRef
Specific Discrimination of Three Pathogenic Salmonella enterica subsp. enterica Serotypes by carB -Based Oligonucleotide Microarray
Hwa Hui Shin, Byeong Hee Hwang, Jeong Hyun Seo, Hyung Joon Cha
Applied and Environmental Microbiology.2014; 80(1): 366. CrossRef
Protective role of gut commensal microbes against intestinal infections
My Young Yoon, Keehoon Lee, Sang Sun Yoon
Journal of Microbiology.2014; 52(12): 983. CrossRef
Comparison of complete rpoB gene sequence typing and multi-locus sequence typing for phylogenetic analysis of Staphylococcus aureus
Won-Jin Seong, Jae-Hong Kim, Hyuk-Joon Kwon
The Journal of General and Applied Microbiology.2013; 59(5): 335. CrossRef

Functional Analysis of the Invariant Residue G791 of Escherichia coli 16S rRNA: Woo-Seok Song , Hong-Man Kim , Jae-Hong Kim , Se-Hoon Sim , Sang-Mi Ryou , Sanggoo Kim , Chang-Jun Cha , Philip R. Cunningham , Jeehyeon Bae , Kangseok Lee; J. Microbiol. 2007;45(5):418-421.; DOI: https://doi.org/2595 [pii]

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Abstract PDF: The nucleotide at position 791(G791) of E. coli 16S rRNA was previously identified as an invariant residue for ribosomal function. In order to characterize the functional role of G791, base substitutions were introduced at this position, and mutant ribosomes were analyzed with regard to their protein synthesis ability, via the use of a specialized ribosome system. These ribosomal RNA mutations attenuated the ability of ribosomes to conduct protein synthesis by more than 65%. A transition mutation (G to A) exerted a moderate effect on ribosomal function, whereas a transversion mutation (G to C or U) resulted in a loss of protein synthesis ability of more than 90%. The sucrose gradient profiles of ribosomes and primer extension analysis showed that the loss of protein-synthesis ability of mutant ribosomes harboring a base substitution from G to U at position 791 stems partially from its inability to form 70S ribosomes. These findings show the involvement of the nucleotide at position 791 in the association of ribosomal subunits and protein synthesis steps after 70S formation, as well as the possibility of using 16S rRNA mutated at position 791 for the selection of second-site revertants in order to identify ligands that interact with G791 in protein synthesis.

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