- Paenibacillus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter
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Soo-Jin Kim , Hayoung Cho , Jae-Hyung Ahn , Hang-Yeon Weon , Jae-Ho Joa , Jeong-Seon Kim , Soon-Wo Kwon
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J. Microbiol. 2019;57(10):836-841. Published online June 27, 2019
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DOI: https://doi.org/10.1007/s12275-019-9118-3
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A Gram-stain-positive, rod-shaped, non-endospore-forming,
motile by means of peritrichous flagella, facultatively anaerobic
bacterium designated TI45-13arT was isolated from Nuruk,
a Korean traditional Makgeolli fermentation starter. It grew
at 4–35°C (optimum, 28–30°C), pH 5.0–9.0 (optimum, pH
7.0) and NaCl concentrations up to 5% (w/v). Phylogenetic
trees generated using 16S rRNA gene sequences revealed that
strain TI45-13arT belonged to the genus Paenibacillus and
showed the highest sequence similarities with Paenibacillus
kyungheensis DCY88T (98.5%), Paenibacillus hordei RH-N24T
(98.4%) and Paenibacillus nicotianae YIM h-19T (98.1%). The
major fatty acid was anteiso-C15:0. The DNA G+C content
was 39.0 mol%, and MK-7 was the predominant isoprenoid
quinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
phosphatidylethanolamine, three unidentified
glycolipids, and one unidentified aminoglycolipid. The
cell-wall peptidoglycan contained meso-diaminopimelic acid.
On the basis of polyphasic taxonomy study, it was suggested
that strain TI45-13arT represents a novel species within the genus
Paenibacillus for which the name Paenibacillus nuruki
sp. nov. is proposed. The type strain was TI45-13arT (= KACC
18728T = NBRC 112013T).
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Citations
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- Anti-aging potential of the endophyte Paenibacillus nuruki in a Saccharomyces cerevisiae lifespan model
Yancho Zarev, Ivan Ivanov, Martin Dimitrov, Iliana Ionkova, Teodora Todorova Pharmacia.2026;[Epub] CrossRef - Metagenomic and meta-metabolomic analysis of traditional Korean rice vinegar productions shows a large variability between producers
Louise Vermote, Byung Hee Chun, Shehzad Abid Khan, Luc De Vuyst, Che Ok Jeon, Stefan Weckx International Journal of Food Microbiology.2025; 440: 111283. CrossRef -
Paenibacillus dendrobii sp. nov., an indole-3-acetic acid-producing endophytic bacterium isolated from Dendrobium nobile
Yadong Hu, Hongjie Li, Yaoyi Chen, Qiling Zhang, Shigang Zheng, Dan Rao, Ze Chun, Ruoxi Zhao
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Makgeolli - The Traditional Choice of Korean Fermented Beverage from Cereal: An Overview on its Composition and Health Benefits
Ganesh SHIMOGA, Sang-Youn KIM Food Science and Technology.2022;[Epub] CrossRef - Integrative Metagenomics–Metabolomics for Analyzing the Relationship Between Microorganisms and Non-volatile Profiles of Traditional Xiaoqu
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Jae-Yun Lee, Woorim Kang, Pil Soo Kim, So-Yeon Lee, Na-Ri Shin, Hojun Sung, June-Young Lee, Ji-Hyun Yun, Yun-Seok Jeong, Jeong Eun Han, Mi-Ja Jung, Dong-Wook Hyun, Hyun Sik Kim, Euon Jung Tak, Jin-Woo Bae International Journal of Systematic and Evolutionary Microbiology.2020; 70(10): 5439. CrossRef
- Soil pH and electrical conductivity are key edaphic factors shaping bacterial communities of greenhouse soils in Korea
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Jeong Myeong Kim , An-Sung Roh , Seung-Chul Choi , Eun-Jeong Kim , Moon-Tae Choi , Byung-Koo Ahn , Sun-Kuk Kim , Young-Han Lee , Jae-Ho Joa , Seong-Soo Kang , Shin Ae Lee , Jae-Hyung Ahn , Jaekyeong Song , Hang-Yeon Weon
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J. Microbiol. 2016;54(12):838-845. Published online November 26, 2016
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DOI: https://doi.org/10.1007/s12275-016-6526-5
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1,094
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Abstract
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Soil microorganisms play an essential role in soil ecosystem
processes such as organic matter decomposition, nutrient
cycling, and plant nutrient availability. The land use for greenhouse
cultivation has been increasing continuously, which
involves an intensive input of agricultural materials to enhance
productivity; however, relatively little is known about
bacterial communities in greenhouse soils. To assess the effects
of environmental factors on the soil bacterial diversity
and community composition, a total of 187 greenhouse soil
samples collected across Korea were subjected to bacterial
16S rRNA gene pyrosequencing analysis. A total of 11,865
operational taxonomic units at a 97% similarity cutoff level
were detected from 847,560 sequences. Among nine soil factors
evaluated; pH, electrical conductivity (EC), exchangeable
cations (Ca2+, Mg2+, Na+, and K+), available P2O5, organic
matter, and NO3-N, soil pH was most strongly correlated
with bacterial richness (polynomial regression, pH: R2 =
0.1683, P < 0.001) and diversity (pH: R2 = 0.1765, P < 0.001).
Community dissimilarities (Bray-Curtis distance) were positively
correlated with Euclidean distance for pH and EC
(Mantel test, pH: r = 0.2672, P < 0.001; EC: r = 0.1473, P < 0.001). Among dominant phyla (> 1%), the relative abundances
of Proteobacteria, Gemmatimonadetes, Acidobacteria,
Bacteroidetes, Chloroflexi, and Planctomycetes were also more
strongly correlated with pH and EC values, compared with
other soil cation contents, such as Ca2+, Mg2+, Na+, and K+.
Our results suggest that, despite the heterogeneity of various
environmental variables, the bacterial communities of
the intensively cultivated greenhouse soils were particularly
influenced by soil pH and EC. These findings therefore shed
light on the soil microbial ecology of greenhouse cultivation,
which should be helpful for devising effective management
strategies to enhance soil microbial diversity and improving
crop productivity.
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- Comparative analysis of bacterial diversity in the rhizosphere of tomato by culture-dependent and -independent approaches
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Shin Ae Lee , Jiyoung Park , Bora Chu , Jeong Myeong Kim , Jae-Ho Joa , Mee Kyung Sang , Jaekyeong Song , Hang-Yeon Weon
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J. Microbiol. 2016;54(12):823-831. Published online November 26, 2016
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DOI: https://doi.org/10.1007/s12275-016-6410-3
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Abstract
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The microbiome in the rhizosphere–the region surrounding
plant roots–plays a key role in plant growth and health,
enhancing nutrient availability and protecting plants from
biotic and abiotic stresses. To assess bacterial diversity in the
tomato rhizosphere, we performed two contrasting approaches:
culture-dependent and -independent. In the culturedependent
approach, two culture media (Reasoner’s 2A agar
and soil extract agar) were supplemented with 12 antibiotics
for isolating diverse bacteria from the tomato rhizosphere
by inhibiting predominant bacteria. A total of 689 bacterial
isolates were clustered into 164 operational taxonomic units
(OTUs) at 97% sequence similarity, and these were found to
belong to five bacterial phyla (Proteobacteria, Actinobacteria,
Bacteroidetes, Acidobacteria, and Firmicutes). Of these, 122
OTUs were retrieved from the antibiotic-containing media,
and 80 OTUs were recovered by one specific antibiotic-containing
medium. In the culture-independent approach, we
conducted Illumina MiSeq amplicon sequencing of the 16S
rRNA gene and obtained 19,215 high-quality sequences, which
clustered into 478 OTUs belonging to 16 phyla. Among the
total OTUs from the MiSeq dataset, 22% were recovered in
the culture collection, whereas 41% of OTUs in the culture
collection were not captured by MiSeq sequencing. These
results showed that antibiotics were effective in isolating
various taxa that were not readily isolated on antibiotic-free
media, and that both contrasting approaches provided complementary
information to characterize bacterial diversity
in the tomato rhizosphere.
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Jeong Myeong Kim, Shin Ae Lee, Hayoung Cho, Soo-Jin Kim, Jae-Ho Joa, Soon-Wo Kwon, Hang-Yeon Weon
International Journal of Systematic and Evolutionary Microbiology
.2017; 67(10): 3728. CrossRef
- Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus
-
Shin Ae Lee , Jeong Myeong Kim , Jae-Hyung Ahn , Jae-Ho Joa , Soo-Jin Kim , Mee-Kyung Sang , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon
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J. Microbiol. 2016;54(11):709-712. Published online October 29, 2016
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DOI: https://doi.org/10.1007/s12275-016-6451-7
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936
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0
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3
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Abstract
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Strain 63MJ-2T was isolated from the feces of broad-winged
katydid (Pseudorhynchus japonicus) collected in Korea. The
16S rRNA gene sequence of this strain showed the highest
sequence similarity with that of Siphonobacter aquaeclarae
P2T (96.1%) and had low similarities (below 86.3%) with those
of other members of family ‘Flexibacteraceae’. The strain
63MJ-2T is a strictly aerobic, Gram-stain-negative, non-motile,
rod-shaped bacterium. The strain grew at 4–35°C (optimum,
25–30°C), pH of 5.0–9.0 (optimum, 6.0–7.0), and
0–2.0% (optimum, 1.0–2.0) (w/v) NaCl. The DNA G+C content
of strain 63MJ-2T was 43.5 mol%. The major fatty acids
were C16:1 ω5c (42.5%), iso-C17:0 3-OH (18.7%), and summed
feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c, 18.0%). The major
menaquinone was MK-7 and polar lipids were phosphatidylethanolamine,
six unknown aminolipids, and five unknown
lipids. Based on the evidence from our polyphasic
taxonomic study, we conclude that strain 63MJ-2T should be
classified as a novel species of the genus Siphonobacter, and
propose the name Siphonobacter intestinalis sp. nov. The
type strain is 63MJ-2T (=KACC 18663T =NBRC 111883T).
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Citations
Citations to this article as recorded by 
- Notification of changes in taxonomic opinion previously published outside the IJSEM: List of Changes in Taxonomic Opinion no. 43
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2026;[Epub] CrossRef - Validation List no. 227: valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2026;[Epub] CrossRef -
Siphonobacter curvatus sp. nov., isolated from a freshwater river
Yunho Lee, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology
.2018; 68(6): 1925. CrossRef
- Characterization of the Bacterial and Archaeal Communities in Rice Field Soils Subjected to Long-Term Fertilization Practices
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Jae-Hyung Ahn , Jaekyeong Song , Byung-Yong Kim , Myung-Sook Kim , Jae-Ho Joa , Hang-Yeon Weon
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J. Microbiol. 2012;50(5):754-765. Published online November 4, 2012
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DOI: https://doi.org/10.1007/s12275-012-2409-6
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781
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2
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123
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Abstract
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The bacterial and archaeal communities in rice field soils subjected to different fertilization regimes for 57 years were investigated in two different seasons, a non-planted, drained season (April) and a rice-growing, flooded season (August), by performing soil dehydrogenase assay, real-time PCR assay and pyrosequencing analysis. All fertilization regimes increased the soil dehydrogenase activity while the abundances of bacteria and archaea increased in the plots receiving inorganic fertilizers plus compost and not in those receiving inorganic fertilizers only. Rice-growing and flooding decreased the soil dehydrogenase activity while they increased the bacterial diversity in rice field soils. The bacterial communities were dominated by Chloroflexi, Proteobacteria, and Actinobacteria and the archaeal communities by Crenarchaeota at the phylum level. In principal coordinates analysis based on the weighted Fast UniFrac metric, the bacterial and archaeal communities were separated primarily by season, and generally distributed along with soil pH, the variation of which had been caused by long-term fertilization. Variations in the relative abundance according to the season or soil pH were observed for many bacterial and archaeal groups. In conclusion, the microbial activity, prokaryotic abundance and diversity, and prokaryotic community structure in the rice field soils were changed by season and long-term fertilization.
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- Gaetbulibacter jejuensis sp. nov., Isolated from Seawater
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You-Sung Oh , Sang-Bin Lim , Hyung-Yeel Kahng , Byoung-Jun Yoon , Duck-Chul Oh , Jae-Ho Joa , Dong-Heon Lee
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J. Microbiol. 2010;48(3):307-311. Published online June 23, 2010
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DOI: https://doi.org/10.1007/s12275-010-9232-8
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A novel marine bacterium, designated strain CNURIC014T was isolated from coastal seawater of Jeju Island in Korea. Strain CNURIC014T formed yellow colonies on marine agar 2216 and the cells were Gram-negative, non-motile, strictly aerobic, rod-shaped. The temperature, pH and NaCl ranges for growth were 15-37°C, pH 6.0-9.0 and 1.0-7.0% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNURIC014T was most closely related to Gaetbulibacter marinus and Gaetbulibacter saemankumensis, with a sequence similarity of 95.1% and 94.6%, respectively. The DNA G+C content of the strain was 33.1 mol% and the major respiratory quinone was menaquinone-6. The major cellular fatty acids were iso-C15:1 (22.8%), iso-C15:0 (18.8%), summed feature 3 (iso-C15:0 2-OH/C16:1 ω7c, 12.9%) and iso-C17:0 3-OH (11.5%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC014T represents a novel species within the
genus Geatbulibacter, for which the name Gaetbulibacter jejuensis sp. nov. is proposed. The type strain is CNURIC014T (=KCTC 22615T =JCM 15976T).
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