- Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli
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Su-Mi Choi , Jae-Ho Jeong , Hyon E. Choy , Minsang Shin
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J. Microbiol. 2016;54(8):559-564. Published online August 2, 2016
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DOI: https://doi.org/10.1007/s12275-016-6027-6
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 Abstract
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Enteropathogenic E. coli causes attaching and effacing (A/E)
intestinal lesions. The genes involved in the formation of A/E
lesions are encoded within a chromosomal island comprising
of five major operons, LEE1-5. The global regulator H-NS
represses the expression of these operons. Ler, a H-NS homologue,
counteracts the H-NS–mediated repression. Using a
novel genetic approach, we identified the amino acid residues
in Ler that are involved in the interaction with H-NS: I20 and
L23 in the C-terminal portion of α-helix 3, and I42 in the
following unstructured linker region.
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Citations
Citations to this article as recorded by  - Structural basis of the oligomerization of anti-silencer Ler from enterohemorrhagic E. coli (EHEC)
Guanghao Wang, Bo Duan, Bin Xia
Structural Dynamics.2026;[Epub] CrossRef - Regulation of the Locus of Enterocyte Effacement in Attaching and Effacing Pathogens
R. Christopher D. Furniss, Abigail Clements, William Margolin
Journal of Bacteriology.2018;[Epub] CrossRef
- Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription
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Hyung-Ju Lim , Kwangsoo Kim , Minsang Shin , Jae-Ho Jeong , Phil Youl Ryu , Hyon E. Choy
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J. Microbiol. 2015;53(4):250-255. Published online April 8, 2015
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DOI: https://doi.org/10.1007/s12275-015-4681-8
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σ38 in Escherichia coli is required for expression of a subset
of stationary phase genes. However, the promoter elements
for σ38-dependent genes are virtually indistinguishable from
that for σ70-dependent house-keeping genes. hdeABp is a
σ38-dependent promoter and LEE5p is a σ70-dependent
promoter, but both are repressed by H-NS, a bacterial histone-
like protein, which acts at promoter upstream sequence.
We swapped the promoter upstream sequences of the two
promoters and found that the σ dependency was switched.
This was further verified using lacUV5 core promoter. The
results
suggested that the determinant for σ38-dependent
promoter lies in the promoter upstream sequence.
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Citations
Citations to this article as recorded by - Sequence-dependent model of genes with dual σ factor preference
Ines S.C. Baptista, Vinodh Kandavalli, Vatsala Chauhan, Mohamed N.M. Bahrudeen, Bilena L.B. Almeida, Cristina S.D. Palma, Suchintak Dash, Andre S. Ribeiro
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms.2022; 1865(3): 194812. CrossRef - Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment
Jun Li, Fengmei Yi, Guoqing Chen, Fanda Pan, Yang Yang, Ming Shu, Zeyu Chen, Zeling Zhang, Xiaotong Mei, Weihong Zhong
Applied Biochemistry and Biotechnology.2021; 193(9): 2793. CrossRef - Recent advances in genetic engineering tools based on synthetic biology
Jun Ren, Jingyu Lee, Dokyun Na
Journal of Microbiology.2020; 58(1): 1. CrossRef
- Immune Response Induced by ppGpp-Defective Salmonella enterica serovar Gallinarum in Chickens
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Sang-Ik Park , Jae-Ho Jeong , Hyon E. Choy , Joon Haeng Rhee , Hee-Sam Na , Tae-Hoon Lee , Moon Her , Kyoung-Oh Cho , Yeongjin Hong
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J. Microbiol. 2010;48(5):674-681. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0179-6
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Abstract
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To protect chickens from typhoid caused by Salmonella enterica serovar Gallinarum (S. Gallinarum), the attenuated 9R strain has been used in the field as a vaccine. However, safety concerns have been raised because the mutations in 9R are undefined while its efficacy is still a question under debate. A global regulator, ppGpp, synthesized by RelA and SpoT, has been shown to induce various virulence genes in S. Gallinarum (Jeong et al., 2008). In this study, two mutant strains defective in ppGpp-synthesis were constructed in wild-type S. Gallinarum (∆ppGpp) and 9R strain (9R-∆ppGpp) backgrounds and tested as live vaccines in chickens. After oral inoculation, the LD50 values of ∆ppGpp and 9R-∆ppGpp were approximately 5×1010 colony forming unit (CFU) similarly as 9R strain, which was ~105-fold higher than that of the wildtype S. Gallinarum strain. Immunological analyses revealed immunization with either of the two attenuated ppGpp-defective strains induced significant antibody responses, the production of antibody-secreting B cells in blood, proliferation of CD4+ and CD8+ T cells in the spleen, and splenic expression of proinflammatory cytokines, such as IFN-γ and TGF-β4, at levels comparable to the 9R strain. Chickens immunized with the mutants (1×108 CFU) were 80% protected against oral challenge with 1×109 wild-type virulent bacteria (4,000-fold LD50 dose), similar to the level of protection achieved by 9R immunization. Based on these data, live attenuated ∆ppGpp-defective strains may serve as novel vaccines to control fowl typhoid in chickens.
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Citations
Citations to this article as recorded by - Progress in the application of Salmonella vaccines in poultry: A mini review
Jie Pan, Rong-rong Wei, Ping Xu, Yun-ying Liu, Chen Li, Guo-wei Ding, Juan Fan, Yu-he Li, Jing-yi Yu, Peng Dai
Veterinary Immunology and Immunopathology.2024; 278: 110855. CrossRef - Mutating both relA and spoT of enteropathogenic Escherichia coli E2348/69 attenuates its virulence and induces interleukin 6 in vivo
Jun Bong Lee, Se Kye Kim, Dalmuri Han, Jang Won Yoon
Frontiers in Microbiology.2023;[Epub] CrossRef - Vaccines to Control Salmonella in Poultry
Roy Curtiss
Avian Diseases.2023;[Epub] CrossRef - Pathophysiology of enteropathogenic Escherichia coli during a host infection
Jun Bong Lee, Se Kye Kim, Jang Won Yoon
Journal of Veterinary Science.2022;[Epub] CrossRef - Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
Jun-Feng Zhang, Ke Shang, Bai Wei, Yea-Jin Lee, Jong-Yeol Park, Hyung-Kwan Jang, Se-Yeoun Cha, Min Kang
Frontiers in Veterinary Science.2021;[Epub] CrossRef - Survival of the Fittest: The Relationship of (p)ppGpp With Bacterial Virulence
Shivani Kundra, Cristina Colomer-Winter, José A. Lemos
Frontiers in Microbiology.2020;[Epub] CrossRef - Immunogenicity and duration of immunity of the polyvalent vaccine against chicken salmonellosis
О. М. Sen, О. О. Saliy, V. I. Mazurkevych, Y. A. Sobko
Regulatory Mechanisms in Biosystems.2020; 11(4): 506. CrossRef - Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Zhili Li, Yuejia Cai, Guozhi Liang, Saeed El-Ashram, Minmin Mei, Wenjing Huang, Xiaowen Li, Wenfeng Li, Cheng He, Shujian Huang
Scientific Reports.2018;[Epub] CrossRef - Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivatedSalmonellaGallinarum vaccine candidate
Gayeon Won, Atul A. Chaudhari, John Hwa Lee
Clinical and Experimental Vaccine Research.2016; 5(2): 148. CrossRef - Characterization of the RelBbu Regulon in Borrelia burgdorferi Reveals Modulation of Glycerol Metabolism by (p)ppGpp
Julia V. Bugrysheva, Christopher J. Pappas, Darya A. Terekhova, Radha Iyer, Henry P. Godfrey, Ira Schwartz, Felipe C. Cabello, Petros C. Karakousis
PLOS ONE.2015; 10(2): e0118063. CrossRef - Generation of a safety enhanced Salmonella Gallinarum ghost using antibiotic resistance free plasmid and its potential as an effective inactivated vaccine candidate against fowl typhoid
Chetan V. Jawale, Atul A. Chaudhari, John Hwa Lee
Vaccine.2014; 32(9): 1093. CrossRef - A novel approach for the generation of Salmonella Gallinarum ghosts and evaluation of their vaccine potential using a prime-booster immunization strategy
Chetan V. Jawale, John Hwa Lee
Vaccine.2014; 32(50): 6776. CrossRef - A review of vaccine development and research for industry animals in Korea
Nak-Hyung Lee, Jung-Ah Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
Clinical and Experimental Vaccine Research.2012; 1(1): 18. CrossRef - Improved vaccines through targeted manipulation of the body's immunological risk‐assessment?
Leif E. Sander
BioEssays.2012; 34(10): 876. CrossRef - Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ PR/GeneEExpression System
Chetan V. Jawale, Atul A. Chaudhari, Byung Woo Jeon, Rahul M. Nandre, John Hwa Lee, A. J. Bäumler
Infection and Immunity.2012; 80(4): 1502. CrossRef - Role of RelA and SpoT in Burkholderia pseudomallei Virulence and Immunity
Claudia M. Müller, Laura Conejero, Natasha Spink, Matthew E. Wand, Gregory J. Bancroft, Richard W. Titball, A. Camilli
Infection and Immunity.2012; 80(9): 3247. CrossRef - Construction of a Salmonella Gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens
Atul A Chaudhari, Chetan V Jawale, Sam Woong Kim, John Hwa Lee
Veterinary Research.2012;[Epub] CrossRef
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