Journal of Microbiology

Jae Su Kim

3 Articles

Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant: Xue Ying Tao , Jae Young Choi , Yong Wang , Jong Yul Roh , Joo Hyun Lee , Qin Liu , Jong Bin Park , Jae Su Kim , Woojin Kim , Yeon Ho Je; J. Microbiol. 2013;51(4):515-521. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3058-0

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ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the nucleocapsids were singly enveloped in the polyhedrin matrix while the nucleocapsids of AcMNPV are known to be multiply enveloped. Furthermore, Ac43KO led to a defect in the transcription and expression of polyhedrin, which resulted in reduced occlusion body production. However, Ac43KO did not affect production of budded virus as there was no remarkable difference in budded virus titer. These results suggest that ac43 plays an important role in the expression of polyhedrin, the morphogenesis of occlusion body, and the assembly of virions occluded in occlusion bodies.

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Systematic analysis of nuclear localization of Autographa californica multiple nucleopolyhedrovirus proteins
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Journal of General Virology.2021;[Epub] CrossRef
The autographa californica multiple nucleopolyhedrovirus Ac12: A non-essential F box-like protein that interacts with cellular SKP1 component of the E3 ubiquitin ligase complex
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Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins
Zhong-Jian Guo, Meng-Han Yu, Xian-Yun Dong, Wei-Li Wang, Ting Tian, Xian-Yin Yu, Xu-Dong Tang
Scientific Reports.2017;[Epub] CrossRef
Disruption of the baculovirus core gene ac78 results in decreased production of multiple nucleocapsid-enveloped occlusion-derived virions and the failure of primary infection in vivo
Sai-Nan Li, Jin-Yu Wang, Mei-Jin Yuan, Kai Yang
Virus Research.2014; 191: 70. CrossRef
The Trichoplusia ni single nucleopolyhedrovirus tn79 gene encodes a functional sulfhydryl oxidase enzyme that is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the sulfhydryl oxidase ac92 gene
Stian A. Clem, Wenbi Wu, A. Lorena Passarelli
Virology.2014; 460-461: 207. CrossRef
Baculovirus nucleocapsid aggregation (MNPV vs SNPV): an evolutionary strategy, or a product of replication conditions?
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Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum: Qin Liu , Jong Yul Roh , Yong Wang , Jae Young Choi , Xue Ying Tao , Jae Su Kim , Yeon Ho Je; J. Microbiol. 2012;50(5):874-877. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2201-7

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Abstract PDF

A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.

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Research progress on the microbiota in bladder cancer tumors
Keyuan Lou, Junpeng Chi, Jitao Wu, Jian Ma, Shu Liu, Yuanshan Cui
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Taxonomic hierarchy of the phylum Firmicutes and novel Firmicutes species originated from various environments in Korea
Chi Nam Seong, Joo Won Kang, Ji Hee Lee, So Yeon Seo, Jung Jae Woo, Chul Park, Kyung Sook Bae, Mi Sun Kim
Journal of Microbiology.2018; 56(1): 1. CrossRef
Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria
Zahia Djenane, Farida Nateche, Meriam Amziane, Joaquín Gomis-Cebolla, Fairouz El-Aichar, Hassiba Khorf, Juan Ferré
Toxins.2017; 9(4): 139. CrossRef

Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses: Hyun Na Koo , Jeong Mi Oh , Jae Kyung Lee , Jae Young Choi , Kwang Sik Lee , Jong Yul Roh , Yeon Ho Je , Byung Rae Jin , Sung Sik Yoo , Jae Su Kim , Young In Kim , In Joong Yoon , Soo Dong Woo; J. Microbiol. 2008;46(6):709-719. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0224-x

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Abstract PDF

To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.

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Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016
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BMC Veterinary Research.2018;[Epub] CrossRef
Enhanced expression of the structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) by SUMO fusion
Hyun Na Koo, Sung Min Bae, Soo Dong Woo
International Journal of Industrial Entomology.2016; 32(2): 90. CrossRef
The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation
Baochao Fan, Xing Liu, Juan Bai, Yufeng Li, Qiaoya Zhang, Ping Jiang, Guihong Zhang
PLOS ONE.2015; 10(9): e0138772. CrossRef
Genomic analysis and pathogenic characteristics of Type 2 porcine reproductive and respiratory syndrome virus nsp2 deletion strains isolated in Korea
Hwan-Won Choi, Eeuri Nam, Yoo Jin Lee, Yun-Hee Noh, Seung-Chul Lee, In-Joong Yoon, Hyun-Soo Kim, Shien-Young Kang, Young-Ki Choi, Changhee Lee
Veterinary Microbiology.2014; 170(3-4): 232. CrossRef
Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus
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Journal of Bacteriology and Virology.2011; 41(3): 173. CrossRef

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