- Experimental and Computational Characterization of the Ferric Uptake Regulator from Aliivibrio salmonicida (Vibrio salmonicida)
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Hege Lynum Pedersen , Rafi Ahmad , Ellen Kristin Riise , Hanna-Kirsti Schrøder Leiros , Stefan Hauglid , Sigrun Espelid , Bjørn Olav Brandsdal , Ingar Leiros , Nils-Peder Willassen , Peik Haugen
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J. Microbiol. 2010;48(2):174-183. Published online May 1, 2010
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DOI: https://doi.org/10.1007/s12275-010-9199-5
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The Ferric uptake regulator (Fur) is a global transcription factor that affects expression of bacterial genes in an iron-dependent fashion. Although the Fur protein and its iron-responsive regulon are well studied, there are still important questions that remain to be answered. For example, the consensus Fur binding site also known as the “Fur box” is under debate, and it is still unclear which Fur residues directly interact with the DNA. Our long-term goal is to dissect the biological roles of Fur in the development of the disease cold-water vibriosis, which is caused by the psychrophilic bacteria Aliivibrio salmonicida (also known as Vibrio salmonicida). Here, we have used experimental and computational methods to characterise the Fur protein from A. salmonicida (AS-Fur). Electrophoretic mobility shift assays show that AS-Fur binds to the recently proposed vibrio Fur box consensus in addition to nine promoter regions that contain Fur boxes. Binding appears to be dependent on the number of Fur boxes, and the predicted “strength” of Fur boxes. Finally, structure modeling and molecular dynamics simulations provide new insights into potential AS-Fur–DNA interactions.
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Citations
Citations to this article as recorded by  - Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling
Kristel Berg, Hege Lynum Pedersen, Ingar Leiros
BioMetals.2020; 33(4-5): 169. CrossRef - Construction of afurnull mutant and RNA-sequencing provide deeper global understanding of theAliivibrio salmonicidaFur regulon
Sunniva Katharina Thode, Cecilie Bækkedal, Jenny Johansson Söderberg, Erik Hjerde, Hilde Hansen, Peik Haugen
PeerJ.2017; 5: e3461. CrossRef - Vibrio Iron Transport: Evolutionary Adaptation to Life in Multiple Environments
Shelley M. Payne, Alexandra R. Mey, Elizabeth E. Wyckoff
Microbiology and Molecular Biology Reviews.2016; 80(1): 69. CrossRef - Single Asparagine to Arginine Mutation Allows PerR to Switch from PerR Box to Fur Box
Christelle Caux-Thang, Aubérie Parent, Ramakrishnan Sethu, Arhamatoulaye Maïga, Geneviève Blondin, Jean-Marc Latour, Victor Duarte
ACS Chemical Biology.2015; 10(3): 682. CrossRef - The Fur Transcription Regulator and Fur‐Regulated Genes in Clostridium botulinum A ATCC 3502
Weibin Zhang, Junhua Ma, Chengyuan Zang, Yingying Song, Peipei Liu, Ayman El-Kadi
BioMed Research International.2011;[Epub] CrossRef
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