Journal of Microbiology

Il Kwon Bae

2 Articles

Nomenclature of ISCR1 Elements Capable of Mobilizing Antibiotic Resistance Genes Present in Complex Class 1 Integrons: Seung Ghyu Sohn , Jae Jin Lee , Jae Seok Song , Jung Hun Lee , Ha Ik Sun , Kwang Seung Park , Il Kwon Bae , Jung-Hyun Lee , Byeong Chul Jeong , Sang Hee Lee; J. Microbiol. 2009;47(4):514-516. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0054-5

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The dissemination of many antibiotic resistance genes has arisen among members of the family Enterobacteriaceae. The dissemination mechanism of these antibiotic resistance genes is closely linked with insertion sequence common region 1 (ISCR1). Thus, caution must be taken in clinical settings to prevent further dissemination of these antibiotic resistance genes. A nomenclature system of ISCR1 variants, important for the antibiotic resistance dissemination, was proposed. The proposed system can designate all ISCR1 variants on the basis of the detection time and by considering amino-acid substitution(s) compared with ISCR1a. This nomenclature system of ISCR1 variants can be applied to 19 groups (ISCR1 to ISCR19) of the ISCR family and help some researchers to correctly designate new ISCR subgroups.

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Molecular Characteristics of Carbapenem-Resistant Gram-Negative Bacteria in Southern China
Fen Zheng, Jingjing Sun, Cancan Cheng, Yongyu Rui
Microbial Drug Resistance.2015; 21(2): 178. CrossRef
Novel ISCR1-linked resistance genes found in multidrug-resistant Gram-negative bacteria in southern China
Fengping Wang, Kuihai Wu, Jingjing Sun, Qian Wang, Qing Chen, Shouyi Yu, Yongyu Rui
International Journal of Antimicrobial Agents.2012; 40(5): 404. CrossRef
Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens
Hatch W. Stokes, Michael R. Gillings
FEMS Microbiology Reviews.2011; 35(5): 790. CrossRef

Outbreaks of Imipenem-Resistant Acinetobacter baumannii Producing Carbapenemases in Korea: Seok Hoon Jeong , Il Kwon Bae , Kwang Ok Park , Young Jun An , Seung Ghyu Sohn , Seon Ju Jang , Kwang Hoon Sung , Ki Suk Yang , Kyungwon Lee , Dongeun Young , Sang Hee Lee; J. Microbiol. 2006;44(4):423-431.; DOI: https://doi.org/2410 [pii]

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Abstract PDF: Among 53 Acinetobacter baumannii isolates collected in 2004, nine imipenem-resistant isolates were obtained from clinical specimens taken from patients hospitalized in Busan, Korea. Nine carbapenemase-producing isolates were further investigated in order to determine the mechanisms underlying resistance. These isolates were then analyzed via antibiotic susceptibility testing, microbiological tests of carbapenemase activity, pI determination, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. One outbreak involved seven cases of infection by A. baumannii producing OXA-23 β-lactamase, and was found to have been caused by a single ERIC-PCR clone. During the study period, the other outbreak involved two cases of infection by A. baumannii producing IMP-1 β-lactamase. The two clones, one from each of the outbreaks, were characterized via a modified cloverleaf synergy test and an EDTA-disk synergy test. The isoelectric focusing of the crude bacterial extracts detected nitrocefin-positive bands with pI values of 6.65 (OXA-23) and 9.0 (IMP-1). The PCR amplification and characterization of the amplicons via direct sequencing showed that the clonal isolates harbored blaIMP-1 or blaOXA-23 determinants. The two clones were characterized by a multidrug resistance phenotype that remained unaltered throughout the outbreak. This resistance encompassed penicillins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. These results appear to show that the imipenem resistance observed among nine Korean A. baumannii isolates could be attributed to the spread of an IMP-1- or OXA-23-producing clone. Our microbiological test of carbapenemase activity is a simple method for the screening of clinical isolates producing class D carbapenemase and/or class B metallo-β-lactamase, in order both to determine their clinical impact and to prevent further spread.

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