Journal of Microbiology

Hyung Suk Baik

5 Articles

Molecular epidemiology of norovirus in asymptomatic food handlers in Busan, Korea, and emergence of genotype GII.17: Hee Soo Koo , Mi Ok Lee , Pyeong Tae Ku , Su Jeong Hwang , Dong Ju Park , Hyung Suk Baik; J. Microbiol. 2016;54(10):686-694. Published online September 30, 2016; DOI: https://doi.org/10.1007/s12275-016-6312-4

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The molecular epidemiology of norovirus infections was studied in food handlers without any symptoms from January to December 2015 in Busan city, Korea. A total of 2,174 fecal specimens from asymptomatic food handlers were analyzed, and 2.3% (49/2,174) were norovirus-positive. Fourteen of 335 samples (4.2%) were positive in January; fifteen of 299 samples (5.0%) in February, and seven of 189 samples (3.7%) in December. However, norovirus was rarely detected in other months. From sequencing analysis, 11 genotypes (five GI and six GII genotypes) were detected. Among the 42 capid gene sequences identified, 14 were from the GI genogroup, while 28 were from the GII genogroup. The most commonly detected genotype was GII.17, comprising 15 (35.7%) of positive samples. From January 2012 to December 2015, 5,138 samples were collected from gastroenteritis patients and outbreaks in Busan. The most detected genotype in 2012, 2013, and 2014 was GII.4 (121, 24, and 12 cases, respectively), but in 2015, GII.17 (25 cases) was the most common. The GII.4 genotype was the major cause of acute gastroenteritis from 2012 to 2014, but the GII.17 genotype became the most prevalent cause in 2015. Continued epidemiological surveillance of GII.17 is needed, together with assessment of the risk of norovirus infection.

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Molecular Epidemiology of Norovirus Outbreaks in Food-Catering Facilities in Busan, 2021–2024
Hee-Soo Koo, Hyeon-Jin Kim, Seon-Young Kim, Jung-Hye Choi, Byung-Jun Kim
Journal of Bacteriology and Virology.2025; 55(4): 360. CrossRef
Global prevalence of norovirus gastroenteritis after emergence of the GII.4 Sydney 2012 variant: a systematic review and meta-analysis
Pan Zhang, Cai Hao, Xie Di, Xue Chuizhao, Li Jinsong, Zheng Guisen, Liu Hui, Duan Zhaojun
Frontiers in Public Health.2024;[Epub] CrossRef
Norovirus antivirals: Where are we now?
Natalie E. Netzler, Daniel Enosi Tuipulotu, Peter A. White
Medicinal Research Reviews.2019; 39(3): 860. CrossRef
Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons
Yee Ching Ng, Ye Won Kim, Jeong-Su Lee, Sung Joon Lee, Moon Jung Song
Journal of Microbiology.2018; 56(9): 683. CrossRef
Emerging recombinant noroviruses identified by clinical and waste water screening
Jennifer H. Lun, Joanne Hewitt, Alefiya Sitabkhan, John-Sebastian Eden, Daniel Enosi Tuipulotu, Natalie E. Netzler, Leigh Morrell, Juan Merif, Richard Jones, Bixing Huang, David Warrilow, Kelly-Anne Ressler, Mark J. Ferson, Dominic E. Dwyer, Jen Kok, Will
Emerging Microbes & Infections.2018; 7(1): 1. CrossRef
Epidemiological characteristics of asymptomatic Norovirus infection in a population from oyster (Ostrea rivularis Gould) farms in southern China
Anna Wang, Qiong Huang, Lin Qin, Xianwu Zhong, Hui Li, Rongfeng Chen, Zhuang Wan, Hong Lin, Junhua Liang, Jiansen Li, Yali Zhuang, Yonghui Zhang
Epidemiology and Infection.2018; 146(15): 1955. CrossRef
Ice-associated norovirus outbreak predominantly caused by GII.17 in Taiwan, 2015
Hao-Yuan Cheng, Min-Nan Hung, Wan-Chin Chen, Yi-Chun Lo, Ying-Shih Su, Hsin-Yi Wei, Meng-Yu Chen, Yen-Chang Tuan, Hui-Chen Lin, Hsu-Yang Lin, Tsung-Yen Liu, Yu-Ying Wang, Fang-Tzy Wu
BMC Public Health.2017;[Epub] CrossRef
Current Status of Norovirus Food Poisoning Related to Bivalve Mollusk and Its Control Measures
Mamoru NODA
Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi).2017; 58(1): 12. CrossRef
Occurrence of norovirus infection in an asymptomatic population in Indonesia
Takako Utsumi, Maria Inge Lusida, Zayyin Dinana, Rury Mega Wahyuni, Laura Navika Yamani, Juniastuti, Soetjipto, Chieko Matsui, Lin Deng, Takayuki Abe, Yen Hai Doan, Yoshiki Fujii, Hirokazu Kimura, Kazuhiko Katayama, Ikuo Shoji
Infection, Genetics and Evolution.2017; 55: 1. CrossRef
Foodborne Infectious Diseases Mediated by Inappropriate Infection Control in Food Service Businesses and Relevant Countermeasures in Korea
Jong Myong Park, Young-Hyun You, Hyun-Min Cho, Ji Won Hong, Sa-Youl Ghim
Osong Public Health and Research Perspectives.2017; 8(3): 159. CrossRef

The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids: Jun Seung Lee , Hye Ryun Ryu , Ji Young Cha , Hyung Suk Baik; J. Microbiol. 2015;53(10):725-731. Published online October 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5256-4

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Abstract PDF

Plants produce a wide array of antimicrobial compounds, such as phenolic compounds, to combat microbial pathogens. The hrp PAI is one of the major virulence factors in the plant pathogen, Pseudomonas syringae. A major role of hrp PAI is to disable the plant defense system during bacterial invasion. We examined the influence of phenolic compounds on hrp PAI gene expression at low and high concentrations. There was approximately 2.5 times more hrpA and hrpZ mRNA in PtoDC3000 that was grown in minimal media (MM) supplemented with 10 μM of ortho-coumaric acid than in PtoDC3000 grown in MM alone. On the other hand, a significantly lower amount of hrpA mRNA was observed in bacteria grown in MM supplemented with a high concentration of phenolic compounds. To determine the regulation pathway for hrp PAI gene expression, we performed qRTPCR using gacS, gacA, and hrpS deletion mutants.

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Journal of Plant Nutrition and Soil Science.2024; 187(3): 301. CrossRef
Regulation of the Pseudomonas syringae Type III Secretion System by Host Environment Signals
Megan R. O’Malley, Jeffrey C. Anderson
Microorganisms.2021; 9(6): 1227. CrossRef
Quantification of Viable Cells of Pseudomonas syringae pv. tomato in Tomato Seed Using Propidium Monoazide and a Real-Time PCR Assay
A-li Chai, Hai-yan Ben, Wei-tao Guo, Yan-xia Shi, Xue-wen Xie, Lei Li, Bao-ju Li
Plant Disease.2020; 104(8): 2225. CrossRef
Validation of RT-qPCR Approaches to MonitorPseudomonas syringaeGene Expression During Infection and Exposure to Pattern-Triggered Immunity
Amy Smith, Amelia H. Lovelace, Brian H. Kvitko
Molecular Plant-Microbe Interactions®.2018; 31(4): 410. CrossRef
Multidrug Efflux Pumps at the Crossroad between Antibiotic Resistance and Bacterial Virulence
Manuel Alcalde-Rico, Sara Hernando-Amado, Paula Blanco, José L. Martínez
Frontiers in Microbiology.2016;[Epub] CrossRef
Global Analysis of Type Three Secretion System and Quorum Sensing Inhibition of Pseudomonas savastanoi by Polyphenols Extracts from Vegetable Residues
Carola Biancalani, Matteo Cerboneschi, Francesco Tadini-Buoninsegni, Margherita Campo, Arianna Scardigli, Annalisa Romani, Stefania Tegli, Boris Alexander Vinatzer
PLOS ONE.2016; 11(9): e0163357. CrossRef

Protein-Protein Interactions between Histidine Kinases and Response Regulators of Mycobacterium tuberculosis H37Rv: Ha-Na Lee , Kwang-Eun Jung , In-Jeong Ko , Hyung Suk Baik , Jeong-Il Oh; J. Microbiol. 2012;50(2):270-277. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-2050-4

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Abstract PDF

Using yeast two-hybrid assay, we investigated protein-protein interactions between all orthologous histidine kinase (HK)/response regulator (RR) pairs of M. tuberculosis H37Rv and identified potential protein-protein interactions between a noncognate HK/RR pair, DosT/NarL. The protein interaction between DosT and NarL was verified by phosphotransfer reaction from DosT to NarL. Furthermore, we found that the DosT and DosS HKs, which share considerable sequence similarities to each other and form a twocomponent system with the DosR RR, have different crossinteraction capabilities with NarL: DosT interacted with NarL, while DosS did not. The dimerization domains of DosT and DosS were shown to be sufficient to confer specificity for DosR, and the different cross-interaction abilities of DosS and DosT with NarL were demonstrated to be attributable to variations in the amino acid sequences of the α2-helices of their dimerization domains.

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The Influence of the Nucleotide Sequences of Random Shine-Dalgarno and Spacer Region on Bovine Growth Hormone Gene Expression: Soon-Young Paik , Kyung Soo Ra , Hoon Sik Cho , Kwang Bon Koo , Hyung Suk Baik , Myung Chul Lee , Jong Won Yun , Jang Won Choi; J. Microbiol. 2006;44(1):64-71.; DOI: https://doi.org/2335 [pii]

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Abstract PDF: To investigate the effects of the nucleotide sequences in Shine-Dalgarno (SD) and the spacer region (SD-ATG) on bovine growth hormone (bGH) gene expression, the expression vectors under the control of the T7 promoter (pT7-7 vector) were constructed using bGH derivatives (bGH1 & bGH14) which have different 5''-coding regions and were induced in E. coli BL21(DE3). Oligonucleotides containing random SD sequences and a spacer region were chemically synthesized and the distance between the SD region and the initiation codon were fixed to nine bases in length. The oligonucleotides were annealed and fused to the bGH1 and bGH14 cDNA, respectively. When the bGH gene was induced with IPTG in E. coli BL21(DE3), some clones containing only bGH14 cDNA produced considerable levels of bGH in the range of 6.9% to 8.5% of total cell proteins by SDS-PAGE and Western blot. Otherwise, the bGH was not detected in any clones with bGH1 cDNA. Accordingly, the nucleotide sequences of SD and the spacer region affect on bGH expression indicates that the sequences sufficiently destabilize the mRNA secondary structure of the bGH14 gene. When the free energy was calculated from the transcription initiation site to the +51 nucleotide of bGH cDNA using a program of nucleic acid folding and hybridization prediction, the constructs with values below ‒26.3 kcal/mole (toward minus direction) were not expressed. The constructs with the original sequence of bGH cDNA also did not show any expression, regardless of the free energy values. Thus, the disruption of the mRNA secondary structure may be a major factor regulating bGH expression in the translation initiation process. Accordingly, the first stem-loop among two secondary structures present in the 5''-end region of the bGH gene should be disrupted for the effective expression of bGH.

Characterization, Cloning and Expression of the Ferritin Gene from the Korean Polychaete, Periserrula leucophryna: Byeong Ryong Jeong , Su-Mi Chung , Nam Joo Baek , Kwang Bon Koo , Hyung Suk Baik , Han-Seung Joo , Chung-Soon Chang , Jang Won Choi; J. Microbiol. 2006;44(1):54-63.; DOI: https://doi.org/2336 [pii]

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Abstract PDF: Ferritin is a major eukaryotic protein and in humans is the protein of iron storage. A partial gene fragment of ferritin (255 bp) taken from the total RNA of Periserrula leucophryna, was amplified by RT-PCR using oligonucleotide primers designed from the conserved metal binding domain of eukaryotic ferritin and confirmed by DNA sequencing. Using the 32P-labeled partial ferritin cDNA fragment, 28 different clones were obtained by the screening of the P. leucophryna cDNA library prepared in the Uni-ZAP XR vector, sequenced and characterized. The longest clone was named the PLF (Periserrula leucophryna ferritin) gene and the nucleotide and amino acid sequences of this novel gene were deposited in the GenBank databases with accession numbers DQ207752 and ABA55730, respectively. The entire cDNA of PLF clone was 1109 bp (CDS: 129-653), including a coding nucleotide sequence of 525 bp, a 5''-untranslated region of 128 bp, and a 3''-noncoding region of 456 bp. The 5''-UTR contains a putative iron responsive element (IRE) sequence. Ferritin has an open reading frame encoding a polypeptide of 174 amino acids including a hydrophobic signal peptide of 17 amino acids. The predicted molecular weights of the immature and mature ferritin were calculated to be 20.3 kDa and 18.2 kDa, respectively. The region encoding the mature ferritin was subcloned into the pT7-7 expression vector after PCR amplification using the designed primers and included the initiation and termination codons; the recombinant clones were expressed in E. coli BL21(DE3) or E. coli BL21(DE3)pLysE. SDS-PAGE and western blot analysis showed that a ferritin of approximately 18 kDa (mature form) was produced and that by iron staining in native PAGE, it is likely that the recombinant ferritin is correctly folded and assembled into a homopolymer composed of a single subunit.

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