- Interaction of Acinetobacter baumannii 19606 and 1656-2 with Acanthamoeba castellanii
-
Migma Dorji Tamang , Shukho Kim , Sung-Min Kim , Hyun-Hee Kong , Jungmin Kim
-
J. Microbiol. 2011;49(5):841-846. Published online November 9, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1063-8
-
-
718
View
-
7
Download
-
8
Crossref
-
 Abstract
 PDF
-
Acinetobacter baumannii is virtually avirulent for healthy people but maintains a high virulence among critically ill patients or immuno-compromised individuals. The ability of A. baumannii to adhere to cells and persist on surfaces as biofilms could be central to its pathogenicity. In the present study, we compared the virulence of the A. baumannii 1656-2 clinical strain, which is able to form a thick biofilm, with the virulence of the A. baumannii type strain (ATCC 19606T). Acanthamoeba castellanii, a single-celled organism, was used as the host model system to study the virulence of A. baumannii. Compared to A. baumannii ATCC 19606T, A. baumannii 1656-2 exhibited a higher ability to adhere and invade A. castellanii cells and had a higher killing rate of A. castellanii cells. Furthermore, co-incubation of the amoeba cells and the cell-free supernatant of A. baumannii resulted in the cell death of the amoebae. Heat inactivation or proteinase K treatment of the supernatant did not eliminate its cytotoxicity, suggesting heat stable non-protein factors are responsible for its cytotoxicity to A. castellanii cells. In conclusion, this study for the first time has revealed the capacity of the A. baumannii strain and/or its metabolic products to induce cytotoxicity in A. castellanii cells.
-
Citations
Citations to this article as recorded by  - The intracellular life of Acinetobacter baumannii
Alexandra Maure, Etienne Robino, Charles Van der Henst
Trends in Microbiology.2023; 31(12): 1238. CrossRef - Molecular screening and characterization of Legionella pneumophila associated free-living amoebae in domestic and hospital water systems
Muhammad Atif Nisar, Kirstin E Ross, Melissa H Brown, Richard Bentham, Jason Hinds, Harriet Whiley
Water Research.2022; 226: 119238. CrossRef -
Interaction of Staphylococcus aureus and Acinetobacter baumannii during
In Vitro
β-Lactam Exposure
Nicholas M. Smith, Alexa Ang, Fanny Tan, Katelyn Macias, Sarah James, Jasleen Sidhu, Justin R. Lenhard
Antimicrobial Agents and Chemotherapy.2021;[Epub] CrossRef - Environmental Free-Living Amoebae Can Predate on Diverse Antibiotic-Resistant Human Pathogens
Félix Bornier, Eline Zas, Damien Potheret, Maria-Halima Laaberki, Bénédicte Coupat-Goutaland, Xavier Charpentier, Christopher A. Elkins
Applied and Environmental Microbiology.2021;[Epub] CrossRef - A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors
Qing-Ming Qin, Jianwu Pei, Gabriel Gomez, Allison Rice-Ficht, Thomas A. Ficht, Paul de Figueiredo
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef - Genomic analysis of a Raoultella ornithinolytica strain causing prosthetic joint infection in an immunocompetent patient
Mamadou Beye, Issam Hasni, Piseth Seng, Caroline Michelle, Bernard La Scola, Didier Raoult, Pierre-Edouard Fournier
Scientific Reports.2018;[Epub] CrossRef - Current and Past Strategies for Bacterial Culture in Clinical Microbiology
Jean-Christophe Lagier, Sophie Edouard, Isabelle Pagnier, Oleg Mediannikov, Michel Drancourt, Didier Raoult
Clinical Microbiology Reviews.2015; 28(1): 208. CrossRef - Acanthamoeba and bacteria produce antimicrobials to target their counterpart
Junaid Iqbal, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Parasites & Vectors.2014;[Epub] CrossRef
|
MSK
