- The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export
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DongGeRaMi Moon , Jin-Ah Bae , Hyun Jin Cho , Jin Ho Yoon
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J. Microbiol. 2008;46(4):422-428. Published online August 31, 2008
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DOI: https://doi.org/10.1007/s12275-008-0177-0
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We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.
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Citations
Citations to this article as recorded by  - Unveiling the Plasmodium inositol (pyro)phosphate pathway: Highlighting inositol polyphosphate multikinase as a novel therapeutic target for malaria
Abigail Obuobi, Neils B. Quashie, Nancy Odurowah Duah-Quashie, Jon R. Sayers, Yash Gupta
PLOS One.2025; 20(12): e0338411. CrossRef - Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef - Control of mRNA Export and Translation Termination by Inositol Hexakisphosphate Requires Specific Interaction with Gle1
Abel R. Alcázar-Román, Timothy A. Bolger, Susan R. Wente
Journal of Biological Chemistry.2010; 285(22): 16683. CrossRef - Generation of expression vectors for high-throughput functional analysis of target genes in Schizosaccharomyces pombe
Jiwon Ahn, Chung-Hae Choi, Chang-Mo Kang, Chun-Ho Kim, Hee-Moon Park, Kyung-Bin Song, Kwang-Lae Hoe, Misun Won, Kyung-Sook Chung
The Journal of Microbiology.2009; 47(6): 789. CrossRef
- Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is Essential for Growth and Involved in mRNA Export
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Hyun Jin Cho , Duk Kyung Hwang , Sun Im Jung , Jin Ho Yoon
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J. Microbiol. 2007;45(4):344-349.
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DOI: https://doi.org/2562 [pii]
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Abstract
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We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm.
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