- Phylogenetic Clustering of 4 Prevalent Virulence Genes in Orientia tsutsugamushi Isolates from Human Patients
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Hyuk Chu , Sang-Hee Park , Eun-Ju Kim , Kyu-Jam Hwang , Soo-Kyoung Shim , Sungdo Park , Mi-Yeoun Park
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J. Microbiol. 2010;48(1):124-128. Published online March 11, 2010
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DOI: https://doi.org/10.1007/s12275-009-0267-7
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The pathogenicity of microbes is involved in many kinds of virulence genes. The relationships between these virulence genes and strains are not clear in Orientia tsutsugamushi yet. In this study, we confirmed the presence of the virulence genes and classified into O. tsutsugamushi isolates using phylogenetic analysis of the
virulence genes. We also compared the fatality rates of every isolate via an infection experiment in BALB/c mice using the O. tsutsugamushi isolates, Deajeon03-01, Wonju03-01, and Muju03-01. Moreover, we compared the phylogenetic analysis, in basis with 56 kDa protein sequence which determined from serotype, and virulence genes of O. tsutsugamushi. Our results showed remarkably different fatality rates between Deajeon03-01 and Muju03-01, which are both Boryong strains of O. tsutsugamushi. Also, clustering analyses including these two isolates gave slightly different results depending on whether they were clustered based on virulence genes or on the 56 kDa protein sequences. Consequently, we conclude that fatality rates in O. tsutsugamushi are correlated with differences in both serotypes and virulence genes. We identified some variations within the virulence genes dnaA, virB8, tolR, and trxA among the isolates.
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Citations
Citations to this article as recorded by  - Epidemiological features of scrub typhus and molecular characteristics of Orientia tsutsugamushi in north-central Bangladesh
Sultana Shabnam Nila, Meiji Soe Aung, Nazia Haque, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Sangjukta Roy, Salma Ahmed, Jobyda Khanam, Mohammad Abdul Awal Bin Sayeed, Shashwata Paul, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Nobumichi Kob
IJID Regions.2025; 14: 100571. CrossRef - Analysis of Factors Related to Regional Occurrence Distribution of Scrub Typhus: 2012∼2016
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The Korean Journal of Clinical Laboratory Science.2019; 51(4): 420. CrossRef - Molecular Epidemiology and Genetic Diversity ofOrientia tsutsugamushifrom Patients with Scrub Typhus in 3 Regions of India
George M. Varghese, Jeshina Janardhanan, Sanjay K. Mahajan, David Tariang, Paul Trowbridge, John A.J. Prakash, Thambu David, Sowmya Sathendra, O.C. Abraham
Emerging Infectious Diseases.2015; 21(1): 64. CrossRef - Phylogenetic Analysis of the 56-kDa Type-Specific Protein Genes ofOrientia tsutsugamushiin Central Korea
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Journal of Korean Medical Science.2012; 27(11): 1315. CrossRef - Differences in Clinical Features According to Boryoung and Karp Genotypes of Orientia tsutsugamushi
Dong-Min Kim, Na Ra Yun, Ganesh Prasad Neupane, Sung Heui Shin, So Yeon Ryu, Hee Jung Yoon, Seong Heon Wie, Woo Jin Kim, Chang Youl Lee, Jong Soo Choi, Tae Young Yang, Frank R. DeLeo
PLoS ONE.2011; 6(8): e22731. CrossRef
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