Journal of Microbiology

Hyoung T. Choi

9 Articles

Biochemical Characterization of Chitinase 2 Expressed during the Autolytic Phase of the Inky Cap, Coprinellus congregatus: Yuri Kang , Hyewon Kim , Hyoung T. Choi; J. Microbiol. 2013;51(2):189-193. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2535-9

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Fungal cell walls consist of various glucans and chitin. The inky cap, Coprinellus congregatus, produces mushrooms at 25°C in a regime of 15 h light/9 h dark, and then the mushroom is autolyzed rapidly to generate black liquid droplets in which no cell walls are detected by microscopy. Chitinase cDNA from the mature mushroom tissues of C. congregatus, which consisted of 1,622 nucleotides (chi2), was successfully cloned using the rapid amplification of cDNA ends polymerase chain reaction technique. The deduced 498 amino acid sequence of Chi2 had a conserved catalytic domain as in other fungal chitinase family 18 enzymes. The Chi2 enzyme was purified from the Pichia pastoris expression system, and its estimated molecular weight was 68 kDa. The optimum pH and temperature of Chi2 was pH 4.0 and 35°C, respectively when 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside was used as the substrate. The Km value and Vmax for the substrate A, 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside, was 0.175 mM and 0.16 OD min-1unit-1, respectively.

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Chitin Synthases in Cordyceps militaris: Genome-Wide Gene Identification, Evolutionary Insights, and Life Cycle Transcript Profiling
Shoumian Li, Huihui Ren, Jie Zhang, Shangpai Gao, Zixuan Chen, Guojie Li, Jinghua Tian, Junling Wang, Ming Li, Xiao Li, Caihong Dong
Horticulturae.2024; 10(5): 494. CrossRef
Chitinase Is Involved in the Fruiting Body Development of Medicinal Fungus Cordyceps militaris
Zi-Juan Zhang, Yuan-Yuan Yin, Yao Cui, Yue-Xuan Zhang, Bi-Yang Liu, You-Chu Ma, Yong-Nan Liu, Gao-Qiang Liu
Life.2023; 13(3): 764. CrossRef
Myco-chitinases as versatile biocatalysts for translation of coastal residual resources to eco-competent chito-bioactives
Meenakshi Rajput, Manish Kumar, Nidhi Pareek
Fungal Biology Reviews.2022; 41: 52. CrossRef
Transcriptome analysis of genes associated with autolysis of Coprinus comatus
Hong-Bo Guo, Zhi-Fei Zhang, Jia-Qing Wang, Si-Yu Wang, Ji-Kang Yang, Xi-Yao Xing, Xiao-Jian Qi, Xiao-Dan Yu
Scientific Reports.2022;[Epub] CrossRef
Production of polyclonal antibody against human Neuritin and its application of immunodetection
Na Wang, Yu Wei, Wen Zhang, Xingyi Li, Jingling Zhu, Liya Shan, Chunyan Liu, Wumei Yuan, Jin Huang
Preparative Biochemistry and Biotechnology.2019; 49(3): 209. CrossRef
Identification and characterization of a marine-derived chitinolytic fungus, Acremonium sp. YS2-2
Dawoon Chung, Kyunghwa Baek, Seung Seob Bae, Jaejoon Jung
Journal of Microbiology.2019; 57(5): 372. CrossRef
Lentinula edodes Genome Survey and Postharvest Transcriptome Analysis
Yuichi Sakamoto, Keiko Nakade, Shiho Sato, Kentaro Yoshida, Kazuhiro Miyazaki, Satoshi Natsume, Naotake Konno, Daniel Cullen
Applied and Environmental Microbiology.2017;[Epub] CrossRef
きのこ類のキチン分解酵素と形態形成への寄与
Yuichi SAKAMOTO, Naotake KONNO
KAGAKU TO SEIBUTSU.2017; 55(10): 663. CrossRef
Heterologous Expression and Characterization of a Novel Chitinase (ChiEn1) from Coprinopsis cinerea and its Synergism in the Degradation of Chitin
Xin Niu, Jiang-Sheng Zhou, Yan-Xin Wang, Cui-Cui Liu, Zhong-Hua Liu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2017; 65(32): 6943. CrossRef
Purification, characterization and physiological significance of a chitinase from the pilei ofCoprinopsis cinereafruiting bodies
Yajun Zhou, Liqin Kang, Xin Niu, Jun Wang, Zhonghua Liu, Sheng Yuan, Paolina Garbeva
FEMS Microbiology Letters.2016; 363(12): fnw120. CrossRef
Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap
Yuri Kang, Hyoung T. Choi
The Korean Journal of Microbiology.2016; 52(2): 226. CrossRef
The Modes of Action of ChiIII, a Chitinase from Mushroom Coprinopsis cinerea, Shift with Changes in the Length of GlcNAc Oligomers
Xin Niu, Cui-Cui Liu, Yuan-Jing Xiong, Ming-Mei Yang, Fei Ma, Zhong-Hua Liu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2016; 64(37): 6958. CrossRef
Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
Zhonghua Liu, Xin Niu, Jun Wang, Wenming Zhang, Mingmei Yang, Cuicui Liu, Yuanjing Xiong, Yan Zhao, Siyu Pei, Qin Qin, Yu Zhang, Yuan Yu, Sheng Yuan
Journal of Agricultural and Food Chemistry.2015; 63(43): 9609. CrossRef
Molecular cloning, characterization, and expression analysis of a β-N-acetylhexosaminidase (LeHex20B) from the shiitake mushroom, Lentinula edodes
Naotake Konno, Ayumi Obara, Yuichi Sakamoto
Journal of Wood Science.2015; 61(2): 178. CrossRef
Novel Protease-Resistant Exochitinase (Echi47) from Pig Fecal Environment DNA with Application Potentials in the Food and Feed Industries
Yuchun Liu, Qiaojuan Yan, Shaoqing Yang, Zhengqiang Jiang
Journal of Agricultural and Food Chemistry.2015; 63(27): 6262. CrossRef
Expression and purification of recombinant human neuritin from Pichia pastoris and a partial analysis of its neurobiological activity in vitro
Yunhua Zhang, Shujun Zhang, Lingling Xian, Juan Tang, Jingling Zhu, Lijuan Cui, Shanman Li, Lei Yang, Jin Huang
Applied Microbiology and Biotechnology.2015; 99(19): 8035. CrossRef
Antifungal chitinase against human pathogenic yeasts from Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
Journal of Microbiology.2014; 52(5): 441. CrossRef
Biochemical Characterization of Heterologously Expressed Chitinase 1 (Chi1) from an Inky Cap, Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
The Korean Journal of Microbiology.2013; 49(4): 309. CrossRef

Degradation of Endocrine Disrupting Chemicals by Genetic Transformants with Two Lignin Degrading Enzymes in Phlebia tremellosa: Hyunwoo Kum , Sungsuk Lee , Sunhwa Ryu , Hyoung T. Choi; J. Microbiol. 2011;49(5):824-827. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1230-y

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Abstract PDF

A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions. An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain.

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Fungi for the bioremediation of pharmaceutical-derived pollutants: A bioengineering approach to water treatment
Galit Akerman-Sanchez, Keilor Rojas-Jimenez
Environmental Advances.2021; 4: 100071. CrossRef
Complete degradation of bisphenol A and nonylphenol by a composite of biogenic manganese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA
Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li
Chemical Engineering Journal.2019; 362: 897. CrossRef
Laccase production and pellet morphology of Coprinopsis cinerea transformants in liquid shake flask cultures
Martin Rühl, Karin Lange, Ursula Kües
Applied Microbiology and Biotechnology.2018; 102(18): 7849. CrossRef
Fate of Bisphenol A in Terrestrial and Aquatic Environments
Jeongdae Im, Frank E. Löffler
Environmental Science & Technology.2016; 50(16): 8403. CrossRef
Molecular characterization of manganese peroxidases from white-rot fungus Polyporus brumalis
Sun-Hwa Ryu, Boyeong Kim, Myungkil Kim, Jin-Ho Seo
Bioprocess and Biosystems Engineering.2014; 37(3): 393. CrossRef
Protoplast Isolation and Genetic Transformation of Polyporus brumalis
Sun-Hwa Ryu, Myung-Kil Kim
The Korean Journal of Microbiology.2014; 50(4): 372. CrossRef
Enhanced Lignin Biodegradation by a Laccase-Overexpressed White-Rot Fungus Polyporus brumalis in the Pretreatment of Wood Chips
Sun-Hwa Ryu, Myung-Kil Cho, Myungkil Kim, Sang-Min Jung, Jin-Ho Seo
Applied Biochemistry and Biotechnology.2013; 171(6): 1525. CrossRef

Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus: Hyangsoon Lim , Hyoung T. Choi; J. Microbiol. 2010;48(5):706-708. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0272-x

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Abstract PDF

Coprinellus congregatus generates several chitinases during its entire life cycle: at the growing hyphal stage and at the mushroom autolysis stage. We have isolated a chitinase gene (chi1) from the mushroom tissue at the autolysing stage, and constructed a chitinase expression vector to get large amount of enzyme protein. Chitinase 1 (chi1) cDNA was heterologously expressed in Saccharomyces cerevisiae by gal1 promoter. The transformants showed no specific change in growth characteristics under normal growth conditions. However the expression of the gene by the gal1 promoter in the yeast transformants resulted in complete growth inhibition, while laccase expression by the gal1 promoter showed normal growth. The chitinase activities from the transformants were also more than 3 times higher than that of the recipient strain, and the chitinase expression by the real time-PCR also showed increased expression of the chi1 in the yeast transformant. Expression of a chitinase which was produced at the mushroom autolysing stage of C. congregatus resulted in yeast growth inhibition.

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The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris
Palanisamy Athiyaman Balakumaran, Jan Förster, Martin Zimmermann, Jayachandran Charumathi, Andreas Schmitz, Eik Czarnotta, Mathias Lehnen, Suresh Sudarsan, Birgitta E. Ebert, Lars Mathias Blank, Sankaranarayanan Meenakshisundaram
BMC Biotechnology.2016;[Epub] CrossRef
Antifungal chitinase against human pathogenic yeasts from Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
Journal of Microbiology.2014; 52(5): 441. CrossRef
Stipe wall extension of Flammulina velutipes could be induced by an expansin-like protein from Helix aspersa
Hejian Fang, Wenming Zhang, Xin Niu, Zhonghua Liu, Changmei Lu, Hua Wei, Sheng Yuan
Fungal Biology.2014; 118(1): 1. CrossRef
Biochemical Characterization of Heterologously Expressed Chitinase 1 (Chi1) from an Inky Cap, Coprinellus congregatus
Yeeun Yoo, Hyoung T. Choi
The Korean Journal of Microbiology.2013; 49(4): 309. CrossRef
Growth Inhibition of Plant Pathogenic Fungi by a Chitinase of Coprinellus congregatus
Yuri Kang, Hyoung T. Choi
The Korean Journal of Microbiology.2012; 48(4): 325. CrossRef

Enhanced Expression of Chitinase during the Autolysis of Mushroom in Coprinellus congregatus: Hyangsoon Lim , Hyoung T. Choi; J. Microbiol. 2009;47(2):225-228. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0247-3

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Abstract PDF

Fungal cell walls consist of various glucans and chitin. An inky cap, Coprinellus congregatus, produced mushrooms at 25°C in a regime of 15 h light/9 h dark, and then the mushroom was autolyzed rapidly to generate black liquid droplets where no cell wall was detected by microscopy. A chitinase cDNA from the matured mushroom cells of C. congregatus that consisted of 1,541 nucleotides was successfully cloned using the rapid amplification of cDNA ends (RACE)-PCR technique. Its deduced 441 amino acid sequence had the conserved catalytic domain as in other fungal chitinase family 18. Chitinase activity was higher at the matured mushroom stage than primordial and young mushroom stage. When the expression of the cloned chitinase was examined by real-time PCR using the chitinase-specific primers, it was increased more than twice to 20 times during the autolytic process of mushroom than young mushroom or primordial stages, respectively.

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The Modes of Action of ChiIII, a Chitinase from Mushroom Coprinopsis cinerea, Shift with Changes in the Length of GlcNAc Oligomers
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Biochemical characterization of chitinase 2 expressed during the autolytic phase of the inky cap, Coprinellus congregatus
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Biochemical Characterization of Heterologously Expressed Chitinase 1 (Chi1) from an Inky Cap, Coprinellus congregatus
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Growth Inhibition of Plant Pathogenic Fungi by a Chitinase of Coprinellus congregatus
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Enhanced Expression of Laccase during the Degradation of Endocrine Disrupting Chemicals in Trametes versicolor: Yunjung Kim , Sumin Yeo , Hong-Gyu Song , Hyoung T. Choi; J. Microbiol. 2008;46(4):402-407. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-007-0236-y

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Abstract PDF

A putative laccase cDNA from a white-rot basidiomycete, Trametes versicolor, that consisted of 1,769 nucleotides was cloned using the rapid amplification of cDNA ends (RACE)-PCR method. The deduced amino acid sequence had 4 putative copper binding regions, which are common to fungal laccases. In addition, the sequence was 57~97% homologous to sequences of other T. versicolor laccases. Additionally, the expression of laccase and manganese peroxidase in this fungus were both greatly increased under degrading conditions for bisphenol A, nonylphenol and two phthalic esters (benzylbutylphthalate and diethylphthalate), all of which are reportedly endocrine disrupting chemicals (EDCs). Furthermore, the estrogenic activities of the EDCs also decreased rapidly during incubation when examined in a two-hybrid yeast system. Finally, kojic acid inhibited the removal of estrogenic activities generated by bisphenol A and nonylphenol, which confirmed that laccase was involved in the degradation of EDCs in T. versicolor.

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Purification and Characterization of an Intracellular NADH: Quinone Reductase from Trametes versicolor: Sang-Soo Lee , Dong-Soo Moon , Hyoung T. Choi , Hong-Gyu Song; J. Microbiol. 2007;45(4):333-338.; DOI: https://doi.org/2564 [pii]

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Abstract PDF: Intracellular NADH:quinone reductase involved in degradation of aromatic compounds including lignin was purified and characterized from white rot fungus Trametes versicolor. The activity of quinone reductase was maximal after 3 days of incubation in fungal culture, and the enzyme was purified to homogeneity using ion-exchange, hydrophobic interaction, and gel filtration chromatographies. The purified enzyme has a molecular mass of 41 kDa as determined by SDS-PAGE, and exhibits a broad temperature optimum between 20-40°C, with a pH optimum of 6.0. The enzyme preferred FAD as a cofactor and NADH rather than NADPH as an electron donor. Among quinone compounds tested as substrate, menadione showed the highest enzyme activity followed by 1,4-benzoquinone. The enzyme activity was inhibited by CuSO4, HgCl2, MgSO4, MnSO4, AgNO3, dicumarol, KCN, NaN3, and EDTA. Its Km and Vmax with NADH as an electron donor were 23 μM and 101 mM/mg per min, respectively, and showed a high substrate affinity. Purified quinone reductase could reduce 1,4-benzoquinone to hydroquinone, and induction of this enzyme was higher by 1,4-benzoquinone than those of other quinone compounds.

Generation of a Transformant Showing Higher Manganese Peroxidase (Mnp) Activity by Overexpression of Mnp Gene in Trametes versicolor: Sumin Yeo , Nammee Park , Hong-Gyu Song , Hyoung T. Choi; J. Microbiol. 2007;45(3):213-218.; DOI: https://doi.org/2540 [pii]

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Abstract PDF: Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.

Increase of Yeast Survival under Oxidative Stress by the Expression of the Laccase Gene from Coprinellus congregatus: Dongsik Kim , Eunju Kwak , Hyoung T. Choi; J. Microbiol. 2006;44(6):617-621.; DOI: https://doi.org/2466 [pii]

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Abstract PDF: Coprinellus congregatus secreted a laccase isozyme when the culture was transferred to an acidic liquid medium (pH 4.1). The laccase cDNA gene (clac2) was used as a probe for cloning of the genomic laccase gene (lac2) including the promoter (Plac2). The open reading frame (ORF) of lac2 had 526 deduced amino acids and four conserved copper binding domains as other fungal laccases. Recombinant plasmid (pRSlac2p-cDNA) of lac2 cDNA with its own promoter was transformed in Saccharomyces cerevisiae. Expression of the transformed lac2 gene was induced by oxidative stress (H2O2) in yeast and the survival rate of the transformed yeast strain was greatly increased when compared with that of the control strain transformed with pRS316 yeast vector.

Cloning of a Manganese Peroxidase cDNA Gene Repressed by Manganese in Trametes versicolor: Yongho Kim , Sumin Yeo , Joohee Kum , Hong-Gyu Song , Hyoung T. Choi; J. Microbiol. 2005;43(6):569-571.; DOI: https://doi.org/2288 [pii]

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Abstract PDF: White-rot fungi have the following enzyme systems for lignin degradation: laccase, lignin peroxidase and manganese peroxidase. There are other types of peroxidases related to lignin degradation, one of which we have cloned a cDNA gene of manganese-repressed peroxidase (MrP) in Trametes versicolor isolated in South Korea. The mrp transcript level has been decreased by 1 M of Mn2+.

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