- Analysis of Bacterial Diversity in Sponges Collected from Chuuk and Kosrae Islands in Micronesia
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In-Hye Jeong , Kyoung-Ho Kim , Hyi-Seung Lee , Jin-Sook Park
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J. Microbiol. 2014;52(1):20-26. Published online January 4, 2014
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DOI: https://doi.org/10.1007/s12275-014-3619-x
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 Abstract
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The bacteria resident in sponges collected from Chuuk Lagoon
and Kosrae Island of Micronesia were investigated using the
16S rRNA gene PCR-tagged pyrosequencing method. These
sponges were clustered into 5 groups based on their bacterial
composition. Diversity indexes and cumulative rank abundance
curves showed the different compositions of bacterial
communities in the various groups of sponges. Reads related
to the phylum Chloroflexi were observed predominantly
(9.7–68.2%) in 9 sponges of 3 groups and unobserved in the
other 2 groups. The Chloroflexi-containing group had similar
bacterial patterns at the phylum and lower taxonomic levels,
for example, significant proportions of Acidobacteria, Gemmatimonadetes,
SBR1093, and PAUC34f were observed in
most members of this group. The three groups in the Chloroflexi-
containing group, however, showed some minor
differences in the composition and diversity. The other two
groups contained high proportions of Proteobacteria (>87%)
or Bacteroidetes (>61%) and different composition and diversity
compared to the Chloroflexi-containing group and each
other. Four pairs of specimens with the same species showed
similar bacterial profiles, but, the bacteria in sponges were
highly specific at the individual level.
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Citations
Citations to this article as recorded by  - Identification of marine sponge-associated bacteria of the Saint Martin's island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita
Sulav Indra Paul, Md. Mahbubur Rahman, Mohammad Abdus Salam, Md. Arifur Rahman Khan, Md. Tofazzal Islam
Aquaculture.2021; 545: 737156. CrossRef - First Record of Microbiomes of Sponges Collected From the Persian Gulf, Using Tag Pyrosequencing
Akram Najafi, Maryam Moradinasab, Iraj Nabipour
Frontiers in Microbiology.2018;[Epub] CrossRef - Acidobacteriaappear to dominate the microbiome of two sympatric Caribbean Sponges and one Zoanthid
Aileen O’Connor-Sánchez, Adán J Rivera-Domínguez, César De los Santos-Briones, Lluvia K López-Aguiar, Yuri J Peña-Ramírez, Alejandra Prieto-Davo
Biological Research.2014;[Epub] CrossRef
- Bioluminescent Assay of Phospholipase C Using A Luminescent Marine Mutant Bacterium Vibrio harveyi M-17
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Ki Woong Cho , SangJun Mo , Hyi-Seung Lee , Jung-Rae Rho , Jongheon Shin
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J. Microbiol. 2000;38(3):150-155.
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Abstract
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A bioluminescent assay method for detecting the activity of phospholipase C (PLC; phosphatidyl choline cholinephosphohydrolase, EC 3.1.4.3) was developed using bioluminescent marine bacteria. Phospholipase C from Bacillus cereus and sn-1,2-dimyristoyl phosphatidyl choline (DMPC) as a substrate were used in the demonstration, and the produced sn-1,2-dimyristoyl glycerol was further hydrolyzed with lipase from Candida cylidracea. The hydrolyzed myristic acid was quantified using a dark mutant of Vibrio harveyi (designated as M-17). The in vivo light intensity of which was stimulated specifically up to one thousand fold in the presence of myristic acid. The rates of the hydrolysis of the DMPC substrate by the phospholipase measured by the luminescence method were linear with time and the amount of enzyme added. Activity measurement conditions (at 25 C, pH 6.5, 10 min fixed time assay) were established to detect as little as 0.1 mUnit of phospholipase C and 5 nM of myristic acid production.
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