- The Proportion of Non-Aflatoxigenic Strains of the Aspergillus flavus/oryzae Complex from Meju by Analyses of the Aflatoxin Biosynthetic Genes
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Seung-Beom Hong , Mina Lee , Dae-Ho Kim , Soo-Hyun Chung , Hyeon-Dong Shin , Robert A. Samson
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J. Microbiol. 2013;51(6):766-772. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3128-3
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Strains of the Aspergillus flavus/oryzae complex are frequently
isolated from meju, a fermented soybean product, that is used
as the starting material for ganjang (soy sauce) and doenjang
(soybean paste) production. In this study, we examined the
aflatoxin producing capacity of A. flavus/oryzae strains isolated
from meju. 192 strains of A. flavus/oryzae were isolated
from more than 100 meju samples collected from diverse
regions of Korea from 2008 to 2011, and the norB-cypA,
omtA, and aflR genes in the aflatoxin biosynthesis gene
cluster were analyzed. We found that 178 strains (92.7%)
belonged to non-aflatoxigenic group (Type I of norB-cypA,
IB-L-B-, IC-AO, or IA-L-B- of omtA, and AO type of aflR),
and 14 strains (7.3%) belonged to aflatoxin-producible group
(Type II of norB-cypA, IC-L-B+/B- or IC-L-B+ of omtA, and
AF type of aflR). Only 7 strains (3.6%) in the aflatoxin-producible
group produced aflatoxins on Czapek yeast-extract
medium. The aflatoxin-producing capability of A. flavus/
oryzae strains from other sources in Korea were also investigated,
and 92.9% (52/56) strains from air, 93.9% (31/33)
strains from rice straw, 91.7% (11/12) strains from soybean,
81.3% (13/16) strains from corn, 82% (41/50) strains from
peanut, and 73.2% (41/56) strains from arable soil were included
in the non-aflatoxigenic group. The proportion of
non-aflatoxigenicity of meju strains was similar to that of
strains from soybean, air and rice straw, all of which have
an effect on the fermentation of meju. The data suggest that
meju does not have a preference for non-aflatoxigenic or aflatoxin-producible strains of A. flavus/oryzae from the
environment of meju. The non-aflatoxigenic meju strains
are proposed to be named A. oryzae, while the meju strains
that can produce aflatoxins should be referred to A. flavus
in this study.
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Citations
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Dong-Hyun Kim, Dong-Chan Kim, Donggun Seo, Ki-Tae Kim, Sang-Han Lee, Seung-Beom Hong
BMC Genomics.2025;[Epub] CrossRef - Distinction Between Aspergillus oryzae and Aflatoxigenic Aspergillus flavus by Rapid PCR Method Based on the Comparative Sequence Analysis of the Aflatoxin Biosynthesis Gene Cluster
Eunji Jeong, Yoo Jin Kwon, Jeong-Ah Seo
Journal of Fungi.2025; 12(1): 10. CrossRef - Inhibitory Effects of Bacillus subtilis Isolated from Meju (Fermented Soybean Brick) on the Growth of Aspergillus parasiticus
Jong-Gyu Kim, Jeong-Yeong Park
Applied Microbiology.2024; 4(1): 354. CrossRef - Analysis of microbial community and the characterization of Aspergillus flavus in Liuyang Douchi during fermentation
Yi Chen, Pao Li, Weiwei He, Luyan Liao, Bo Xia, Liwen Jiang, Yang Liu
LWT.2022; 154: 112567. CrossRef - Effect of allyl isothiocyanate on transcriptional profile, aflatoxin synthesis, and Aspergillus flavus growth
Tiago de Melo Nazareth, Manuel Alonso-Garrido, Oana Stanciu, Jordi Mañes, Lara Manyes, Giuseppe Meca
Food Research International.2020; 128: 108786. CrossRef - Safety of the fungal workhorses of industrial biotechnology: update on the mycotoxin and secondary metabolite potential of Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei
Jens C. Frisvad, Lars L. H. Møller, Thomas O. Larsen, Ravi Kumar, José Arnau
Applied Microbiology and Biotechnology.2018; 102(22): 9481. CrossRef - A comparative secretome analysis of industrial Aspergillus oryzae and its spontaneous mutant ZJGS-LZ-21
Yuanyuan Zhu, Xinle Liang, Hong Zhang, Wei Feng, Ye Liu, Fuming Zhang, Robert J Linhardt
International Journal of Food Microbiology.2017; 248: 1. CrossRef - Mold and Human Health: a Reality Check
Andrea T. Borchers, Christopher Chang, M. Eric Gershwin
Clinical Reviews in Allergy & Immunology.2017; 52(3): 305. CrossRef - Addition of Carbon to the Culture Medium Improves the Detection Efficiency of Aflatoxin Synthetic Fungi
Tadahiro Suzuki, Yumiko Iwahashi
Toxins.2016; 8(11): 338. CrossRef - Mycobiota of ground red pepper and their aflatoxigenic potential
Hyeonheui Ham, Sosoo Kim, Min-Hee Kim, Soohyung Lee, Sung Kee Hong, Jae-Gee Ryu, Theresa Lee
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Perng-Kuang Chang, Leslie L. Scharfenstein, Cesar D. Solorzano, Hamed K. Abbas, Sui-Sheng T. Hua, Walker A. Jones, Robert M. Zablotowicz
International Journal of Food Microbiology.2015; 200: 66. CrossRef - The potential hazards of Aspergillus sp. in foods and feeds, and the role of biological treatment: A review
Sheikh Imranudin Sheikh-Ali, Akil Ahmad, Siti-Hamidah Mohd-Setapar, Zainul Akmal Zakaria, Norfahana Abdul-Talib, Aidee Kamal Khamis, Md Enamul Hoque
Journal of Microbiology.2014; 52(10): 807. CrossRef - Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
Min Sik Kim, Sinil Kim, Byeong-Seok Ha, Hye-Young Park, Seong-Yeol BaeK, Soo-Hwan Yeo, Hyeon-Su Ro
The Korean Journal of Mycology.2014; 42(3): 191. CrossRef
- Mitochondrial Phylogeny Reveals Intraspecific Variation in Peronospora effusa, the Spinach Downy Mildew Pathogen
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Young-Joon Choi , Marco Thines , Jae-Gu Han , Hyeon-Dong Shin
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J. Microbiol. 2011;49(6):1039-1043. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1069-2
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678
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Since about two hundred years, downy mildew caused by Peronospora effusa is probably the most economically important disease of spinach (Spinacia oleracea). However, there is no information on the global phylogeographic structure of the pathogen and thus it is unclear whether a single genotype occurs worldwide
or whether some local genetic variation exists. To investigate the genetic variability of this pathogen, a sequence analysis of two partial mitochondrial DNA genes, cox2 and nad1, was carried out. Thirty-three specimens of Peronospora effusa from four continents were analyzed, including samples from Australia, China, Japan, Korea, Mexico, Russia, Sweden, and the USA. Despite the potential anthropogenic admixture of genotypes, a phylogeographic pattern was observed, which corresponds to two major groups, an Asian/Oceanian clade and another group, which includes American/European specimens. Notably, two of six Japanese specimens investigated did not belong to the Asian/Oceanian clade, but were identical to three of the specimens from the USA, suggestive of a recent introduction from the USA to Japan. As similar introduction events may be occurring as a result of the globalised trade with plant and seed material, a better knowledge of the phylogeographic distribution of pathogens is highly warranted for food security purposes.
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Citations
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Petros Skiadas, Joël Klein, Thomas Quiroz‐Monnens, Joyce Elberse, Ronnie de Jonge, Guido Van den Ackerveken, Michael F. Seidl
Environmental Microbiology.2022; 24(3): 1622. CrossRef - Population structure and migration of the Tobacco Blue Mold Pathogen, Peronospora tabacina, into North America and Europe
Monica Blanco‐Meneses, Ignazio Carbone, Jean B. Ristaino
Molecular Ecology.2018; 27(3): 737. CrossRef - Population Structure of Peronospora effusa in the Southwestern United States
Rebecca Lyon, James Correll, Chunda Feng, Burt Bluhm, Sandesh Shrestha, Ainong Shi, Kurt Lamour, Rita Grosch
PLOS ONE.2016; 11(2): e0148385. CrossRef - Evolution of Hyaloperonospora effectors: ATR1 effector homologs from sister species of the downy mildew pathogen H. arabidopsidis are not recognised by RPP1WsB
Irina Solovyeva, Angelika Schmuker, Liliana M. Cano, Mireille van Damme, Sebastian Ploch, Sophien Kamoun, Marco Thines
Mycological Progress.2015;[Epub] CrossRef - Towards a universal barcode of oomycetes – a comparison of the cox1 and cox2 loci
Young‐Joon Choi, Gordon Beakes, Sally Glockling, Julia Kruse, Bora Nam, Lisa Nigrelli, Sebastian Ploch, Hyeon‐Dong Shin, Roger G. Shivas, Sabine Telle, Hermann Voglmayr, Marco Thines
Molecular Ecology Resources.2015; 15(6): 1275. CrossRef - Characterisation and risk assessment of the emerging Peronospora disease on Aquilegia
Geoffrey J. Denton, Elizabeth J. Beal, Anne Kilty, Jennifer O. Denton, Young-Joon Choi, Marco Thines
Mycological Progress.2015;[Epub] CrossRef - Coupling Spore Traps and Quantitative PCR Assays for Detection of the Downy Mildew Pathogens of Spinach (Peronospora effusa) and Beet (P. schachtii)
Steven J. Klosterman, Amy Anchieta, Neil McRoberts, Steven T. Koike, Krishna V. Subbarao, Hermann Voglmayr, Young-Joon Choi, Marco Thines, Frank N. Martin
Phytopathology®.2014; 104(12): 1349. CrossRef
- Sawadaea koelreuteriae comb. nov., a Powdery Mildew of Koelreuteria paniculata
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Hyeon-Dong Shin , Mi-Jeong Park
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J. Microbiol. 2011;49(5):862-866. Published online November 9, 2011
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DOI: https://doi.org/10.1007/s12275-011-1479-1
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720
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A powdery mildew parasitizing Koelreuteria spp. was first described under the name Uncinula koelreuteriae Miyake and later transferred to the genus Typhulochaeta. Based on morphological and molecular data of several herbarium specimens collected from Korea, the generic placement of Typhulochaeta is discussed and T. koelreuteriae is combined in the genus Sawadaea. Redescription and epitypification of this species is provided hereby.
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Citations
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Yu-Wei Yeh, Roland Kirschner
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Forest Pathology.2020;[Epub] CrossRef - Molecular phylogeny reveals the presence of cryptic speciation within Erysiphe japonica (≡Typhulochaeta japonica), a powdery mildew on Quercus spp.
Jamjan Meeboon, Susumu Takamatsu
Mycoscience.2013; 54(1): 69. CrossRef
- A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates
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Mi-Jeong Park , Quinn A. Holtslag , Hyeon-Dong Shin
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J. Microbiol. 2011;49(2):324-326. Published online May 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-1117-y
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613
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Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful
in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and
transferring the characteristic fan-shaped colonies to fresh medium.
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Hao Chen, James F. White, Kamran Malik, Fang Qi, Chunjie Li
Plant Disease.2022; 106(11): 2884. CrossRef - Occurrence of Leaf Spot on Eriobotrya japonica Caused by Entomosporium mespili in Korea
Sang-Tae Seo, Mi-Jeong Park, Hyeon-Dong Shin, Kyung-Hee Kim
Research in Plant Disease.2011; 17(2): 228. CrossRef
- Re-identification of Aspergillus fumigatus sensu lato Based on a New Concept of Species Delimitation
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Seung-Beom Hong , Dae-Ho Kim , In-Cheol Park , Young-Joon Choi , Hyeon-Dong Shin , Robert Samson
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J. Microbiol. 2010;48(5):607-615. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0084-z
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682
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11
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The species concept of Aspergillus fumigatus sensu stricto has recently been defined by polyphasic taxonomy. Based on the new concept of species delimitations, 146 worldwide strains of Aspergillus fumigatus sensu lato were re-identified. Of those 146 strains, 140 (95.8%) could be identified as A. fumigatus sensu stricto, 3 (2.1%) as A. lentulus, and the remaining 3 strains as A. viridinutans complex, Neosartorya udagawae, and N. cf. nishimurae. Of 98 clinical strains, only 1 from dolphin nostril was identified as A. lentulus and not A. fumigatus sensu stricto. Random amplification of polymorphic DNA-polymerase chain reaction (RAPD-PCR) with primers PELF and URP1F produced nearly the same band patterns among 136 strains of A. fumigatus sensu stricto while discriminated the species from its related species. We also discussed about identification of several atypical A. fumigatus strains from clinical environments.
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Gabriela A. Pena, Verónica Alonso, Marina Velasco Manini, M. Pellegrino, Lilia R. Cavaglieri
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