- The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans
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Jong-Myeong Kim , Hye Yun Moon , Dong Wook Lee , Hyun Ah Kang , Jeong-Yoon Kim
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J. Microbiol. 2021;59(10):911-919. Published online September 7, 2021
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DOI: https://doi.org/10.1007/s12275-021-1326-y
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Candida albicans is an opportunistic human pathogen that
exists as yeast, hyphal or pseudohyphal forms depending on
pH, nutrients, and temperature. The morphological transition
from yeast to hyphae, which is required for the complete virulence
of C. albicans, is controlled by many transcription factors
that activate or repress hypha-specific genes. The C. albicans
transcriptional factor Cas5, a key regulator of genes involved
in cell wall integrity, affects the susceptibility of C. albicans
to fluconazole, an inhibitor of ergosterol synthesis. In
this study, we found that deletion of CAS5 in C. albicans decreased
the expression levels of a set of ergosterol biosynthesis
genes, such as ERG2, ERG3, ERG5, ERG6, ERG11, and ERG24,
result
ing in the accumulation of lanosterol and zymosterol,
which are intermediate metabolites in the ergosterol biosynthesis
pathway. Interestingly, it was observed that the cas5Δ/Δ
mutant could not maintain the yeast form under non-hyphainducing
conditions, while the CAS5-overexpressing cells could
not form hyphae under hypha-inducing conditions. Consistent
with these observations, the cas5Δ/Δ mutant highly expressed
hypha-specific genes, ALS3, ECE1, and HWP1, under
non-hypha-inducing conditions. In addition, CAS5 transcription
was significantly downregulated immediately after hyphal
initiation in the wild-type strain. Furthermore, the cas5Δ/Δ
mutant reduced the transcription of NRG1, which encodes
a major repressor of hyphal morphogenesis, while Cas5 overexpression
increased the transcription of NRG1 under hyphainducing
conditions. Collectively, this study suggests the potential
role of Cas5 as a repressor of hypha-specific genes during
yeast-form growth of C. albicans.
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Citations
Citations to this article as recorded by  - Biocontrol effects and mechanisms of two Streptomyces strains against root rot of Codonopsis pilosula caused by Fusarium oxysporum
Wei Wang, Lulu Zhang, Zhanfeng Cao, Weijing Yang, Xingzheng Wang, Yufen Yang, Ziyi Hu, Shufeng Wei, Ning Yang
Physiological and Molecular Plant Pathology.2026; 145: 103378. CrossRef - Cas5 Regulates the Exposure of β-Glucan, the Cell Surface Hydrophobicity, and the Expression of Cell Wall Proteins to Remodel the Candida albicans Cell Wall and Participates in the Recruitment of Neutrophils
Qiyue Zhang, Guanglin Li, Yanmei Wang, Chen Yang, Wenhui Bai, Qingqing Li, Jiye Zhang, Peipei Zhang
Microorganisms.2025; 13(3): 683. CrossRef - The Role of Sfp1 in Candida albicans Cell Wall Maintenance
Che-Kang Chang, Min-Chi Yang, Hsueh-Fen Chen, Yi-Ling Liao, Chung-Yu Lan
Journal of Fungi.2022; 8(11): 1196. CrossRef
- Molecular characterization of the Saccharomycopsis fibuligera ATF genes, encoding alcohol acetyltransferase for volatile acetate ester formation
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Hye Yun Moon , Hyeon Jin Kim , Ki Seung Kim , Su Jin Yoo , Dong Wook Lee , Hee Je Shin , Jeong Ah Seo , Hyun Ah Kang
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J. Microbiol. 2021;59(6):598-608. Published online May 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-1159-8
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1,024
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15
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Abstract
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Aroma ester components produced by fermenting yeast cells
via alcohol acetyltransferase (AATase)-catalyzed intracellular
reactions are responsible for the fruity character of fermented
alcoholic beverages, such as beer and wine. Acetate esters
are reportedly produced at relatively high concentrations by
non-Saccharomyces species. Here, we identified 12 ATF orthologues
(SfATFs) encoding putative AATases, in the diploid
genome of Saccharomycopsis fibuligera KJJ81, an isolate from
wheat-based Nuruk in Korea. The identified SfATF proteins
(SfAtfp) display low sequence identities with S. cerevisiae
Atf1p (between 13.3 and 27.0%). All SfAtfp identified, except
SfAtf(A)4p and SfAtf(B)4p, contained the activation domain
(HXXXD) conserved in other Atf proteins. Culture supernatant
analysis using headspace gas chromatography mass spectrometry
confirmed that the recombinant S. cerevisiae strains
expressing SfAtf(A)2p, SfAtf(B)2p, and SfAtf(B)6p produced
high levels of isoamyl and phenethyl acetates. The volatile
aroma profiles generated by the SfAtf proteins were distinctive
from that of S. cerevisiae Atf1p, implying difference in
the substrate preference. Cellular localization analysis using
GFP fusion revealed the localization of SfAtf proteins proximal
to the lipid particles, consistent with the presence of amphipathic
helices at their N- and C-termini. This is the first
report that systematically characterizes the S. fibuligera ATF
genes encoding functional AATases responsible for acetate
ester formation using higher alcohols as substrate, demonstrating
their biotechnological potential for volatile ester production.
-
Citations
Citations to this article as recorded by - Aroma modulation of wet Spirulina by a microbial consortium: Deciphering the role of community succession and metabolic pathways
Kerui Su, Feihang Wang, Anqi Zhao, Beixiao Zhang, Zhihao Fan, Qi Wang, Xuejing Duan, Hanyu Dong, A.E. Solovchenko, Ayesha Shahid, Jingliang Xu
Food Microbiology.2026; 137: 105043. CrossRef - Two Metschnikowia nectar yeast species have similar volatile profiles but elicit differential foraging in bee pollinators
M. Elizabeth Moore, Lindsey Wilson, Nathan Brandt, Ayako Wada‐Katsumata, Ahmed M. Saveer, Coby Schal, Robert R. Dunn, Rebecca E. Irwin, Caiti Smukowski Heil
Ecological Entomology.2026;[Epub] CrossRef - Starter cultures for sourdough production: rational selection, mixed-strain starter cultures, and strain monitoring
Inés Pradal, Stefan Weckx, Luc De Vuyst
Critical Reviews in Food Science and Nutrition.2026; : 1. CrossRef - Metabolomics-Driven Insights into Rice Wine Fermentation: From Descriptive Profiling to Intelligent Process Control
Baoyu Peng, Bifeng Chen, Zhaozhao Dai, Jinwen Chen, Lang Hu, Lelei Wen, Changchun Li
Fermentation.2026; 12(6): 264. CrossRef - Enhancement of fermentation starter (Jiuyao) quality through reinforced inoculation of indigenous non-Saccharomyces species
Qiang Hu, Xiang Ni, Jingfeng Zha, Jiahao Lin, Yanyun Wang, Nabil Ibrahim Elsheery, Guangfa Xie, Qi Peng
LWT.2026; 255: 119760. CrossRef - Investigating the role of primary fungi in Huangjiu fermentation: Insights from flavor orientation and synthetic microbiomes
Qi Peng, Huihui Zhou, Huajun Zheng, Guangfa Xie
Food Microbiology.2025; 129: 104765. CrossRef - Molecular functional mechanisms of two alcohol acetyltransferases in Lavandula x intermedia (lavandin)
Dafeng Liu, Yanyan Du, Ablikim Abdiriyim, Lvxia Zhang, Daoqi Song, Huashui Deng, Xiongying Wen, Yanyan Zhang, Bingwang Sun
Frontiers in Chemistry.2025;[Epub] CrossRef - Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing
Anastasia E.C. Rumpl, Joshua R. Goodhew, Paul F. Kelly, Mika Hirano, Michael E. Pyne
Biotechnology Advances.2025; 84: 108684. CrossRef - Genome-based exploration of volatile flavor diversity from food yeast species
Su Jin Yoo, Da Eun Kim, Lintang Ignatius Satyawan, Seong-Il Eyun, Che Ok Jeon, Hyun Ah Kang, Zbigniew Lazar
FEMS Yeast Research.2025;[Epub] CrossRef - Genome-Wide Identification and Biochemical Characterization of Alcohol Acyltransferases for Aroma Generation in Wickerhamomyces subpelliculosus Isolates from Fermented Food
Su Jin Yoo, Hyeon Jin Kim, Hye Yun Moon, Min-Seung Jeon, Yong Uk Cho, Che Ok Jeon, Seong-Il Eyun, Hyun Ah Kang
Journal of Agricultural and Food Chemistry.2024; 72(50): 28194. CrossRef -
Characterization and phylogenetic analysis of the complete mitochondrial genome of
Saccharomycopsis fibuligera
(lindner) Klocker 1907 (saccharomycetales: saccharomycopsidaceae)
Yue Deng, Guangjiu Chen, Xuedong Bao, Jie He
Mitochondrial DNA Part B.2024; 9(6): 743. CrossRef - Optimization of High-Density Fermentation Conditions for Saccharomycopsis fibuligera Y1402 through Response Surface Analysis
Hongyang Yuan, Qi Sun, Lanshuang Wang, Zhilei Fu, Tianze Zhou, Jinghao Ma, Xiaoyan Liu, Guangsen Fan, Chao Teng
Foods.2024; 13(10): 1546. CrossRef - Genomic and functional features of yeast species in Korean traditional fermented alcoholic beverage and soybean products
Da Min Jeong, Hyeon Jin Kim, Min-Seung Jeon, Su Jin Yoo, Hye Yun Moon, Eun-joo Jeon, Che Ok Jeon, Seong-il Eyun, Hyun Ah Kang
FEMS Yeast Research.2023;[Epub] CrossRef - Beer fermentation performance and sugar uptake of Saccharomycopsis fibuligera–A novel option for low-alcohol beer
Yvonne Methner, Frederico Magalhães, Luis Raihofer, Martin Zarnkow, Fritz Jacob, Mathias Hutzler
Frontiers in Microbiology.2022;[Epub] CrossRef - Comparative analysis of aroma components and quality of Geotrichum candidum after space mutation breeding
Junjie Chen, Qianying Li, Jie Wang, Weizhe Chen, Qikai Zheng, Qingping Zhong, Xiang Fang, Zhenlin Liao
Frontiers in Microbiology.2022;[Epub] CrossRef
- Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
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Jin Ho Choo , Su-Bin Lee , Hye Yun Moon , Kun Hwa Lee , Su Jin Yoo , Keun Pil Kim , Hyun Ah Kang
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J. Microbiol. 2021;59(2):151-163. Published online February 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-0646-2
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940
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Ogataea parapolymorpha (Hansenula polymorpha DL-1) is
a thermotolerant methylotrophic yeast with biotechnological
applications. Here, O. parapolymorpha genes whose expression
is induced in response to heat shock were identified by
transcriptome analysis and shown to possess heat shock elements
(HSEs) in their promoters. The function of O. parapolymorpha
HSF1 encoding a putative heat shock transcription
factor 1 (OpHsf1) was characterized in the context of heat
stress response. Despite exhibiting low sequence identity
(26%) to its Saccharomyces cerevisiae homolog, OpHsf1 harbors
conserved domains including a DNA binding domain
(DBD), domains involved in trimerization (TRI), transcriptional
activation (AR1, AR2), transcriptional repression (CE2),
and a C-terminal modulator (CTM) domain. OpHSF1 could
complement the temperature sensitive (Ts) phenotype of a
S. cerevisiae hsf1 mutant. An O. parapolymorpha strain with
an H221R mutation in the DBD domain of OpHsf1 exhibited
significantly retarded growth and a Ts phenotype. Intriguingly,
the expression of heat-shock-protein‒coding genes harboring
HSEs was significantly decreased in the H221R mutant
strain, even under non-stress conditions, indicating the importance
of the DBD for the basal growth of O. parapolymorpha.
Notably, even though the deletion of C-terminal domains
(ΔCE2, ΔAR2, ΔCTM) of OpHsf1 destroyed complementation
of the growth defect of the S. cerevisiae hsf1 strain,
the C-terminal domains were shown to be dispensable in O.
parapolymorpha. Overexpression of OpHsf1 in S. cerevisiae
increased resistance to transient heat shock, supporting the
idea that OpHsf1 could be useful in the development of heatshock‒
resistant yeast host strains.
-
Citations
Citations to this article as recorded by - Thermotolerant yeasts and their biotechnological applications
Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny
Trends in Biotechnology.2026; 44(9): 2563. CrossRef - Lignocellulosic biomass fermentation: a roadmap for Candida famata and Ogataea polymorpha
Dominik Wojdyła, Roksolana Vasylyshyn, Alicja Najdecka, Justyna Ruchala
FEMS Yeast Research.2025;[Epub] CrossRef - Sucrose‐Induced Transcriptomic Response in Ogataea polymorpha TBRC 4839 Reveals its Potential for Recombinant Protein Production
Somsak Likhitrattanapisal, Chitwadee Phithakrotchanakoon, Aekkachai Puseenam, Paopit Siriarchawatana, Natta Wiriyakun, Jiraprapa Nirapun, Warasirin Sornlek, Supawadee Ingsriswang, Niran Roongsawang
Yeast.2025; 42(8-10): 181. CrossRef - A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisiae and Escherichia coli
Xinru Ren, Yue Wei, Honglu Zhao, Juanjuan Shao, Fanli Zeng, Zhen Wang, Li Li
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef - Heat shock in Cronobacter sakazakii induces direct protection and cross-protection against simulated gastric fluid stress
Hongmei Niu, MingzheYang, Yonghua Qi, Yangtai Liu, Xiang Wang, Qingli Dong
Food Microbiology.2022; 103: 103948. CrossRef - A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation
Dongxu Shen, Xiaoli He, Peifang Weng, Yanan Liu, Zufang Wu
FEMS Yeast Research.2022;[Epub] CrossRef
- Development of recombinant Yarrowia lipolytica producing virus-like particles of a fish nervous necrosis virus
-
Van-Trinh Luu , Hye Yun Moon , Jee Youn Hwang , Bo-Kyu Kang , Hyun Ah Kang
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J. Microbiol. 2017;55(8):655-664. Published online July 28, 2017
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DOI: https://doi.org/10.1007/s12275-017-7218-5
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1,007
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0
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22
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Abstract
PDF
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Nervous necrosis virus (NNV) causes viral encephalopathy
and retinopathy, a devastating disease of many species of
cultured marine fish worldwide. In this study, we used the
dimorphic non-pathogenic yeast Yarrowia lipolytica as a
host to express the capsid protein of red-spotted grouper
nervous necrosis virus (RGNNV-CP) and evaluated its potential
as a platform for vaccine production. An initial attempt
was made to express the codon-optimized synthetic
genes encoding intact and N-terminal truncated forms of
RGNNV-CP under the strong constitutive TEF1 promoter
using autonomously replicating sequence (ARS)-based vectors.
The full-length recombinant capsid proteins expressed
in Y. lipolytica were detected not only as monomers and
but also as trimers, which is a basic unit for formation of
NNV virus-like particles (VLPs). Oral immunization of mice
with whole recombinant Y. lipolytica harboring the ARSbased
plasmids was shown to efficiently induce the formation
of IgG against RGNNV-CP. To increase the number of
integrated copies of the RGNNV-CP expression cassette, a
set of 26S ribosomal DNA-based multiple integrative vectors
was constructed in combination with a series of defective
Ylura3 with truncated promoters as selection markers, resulting
in integrants harboring up to eight copies of the RGNNVCP
cassette. Sucrose gradient centrifugation and transmission
electron microscopy of this high-copy integrant were
carried out to confirm the expression of RGNNV-CPs as
VLPs. This is the first report on efficient expression of viral
capsid proteins as VLPs in Y. lipolytica, demonstrating high
potential for the Y. lipolytica expression system as a platform
for recombinant vaccine production based on VLPs.
-
Citations
Citations to this article as recorded by - Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides
Seo Hyeon Shin, Hye Yun Moon, Hae Eun Park, Gi Jeong Nam, Ju Hye Baek, Che Ok Jeon, Hyunwook Jung, Myeong Seok Cha, Sol Choi, Jeong Jun Han, Chen Yuan Hou, Chang Seo Park, Hyun Ah Kang
Metabolic Engineering.2025; 87: 68. CrossRef - Sustainable aquaculture and sea ranching with the use of vaccines: a review
Alma Alfatat, Kwaku Amoah, Jia Cai, Yu Huang, Muhammad Fachri, Hagai Nsobi Lauden, Shaoliang Lyu, Xuefeng Wang, Sahya Maulu, Berchie Asiedu, Syaifiuddin Syaifiuddin
Frontiers in Marine Science.2025;[Epub] CrossRef - Yeast vaccine production platform for human and animal infectious diseases
Abel Ramos-Vega, Elizabeth Monreal-Escalante, Bernardo Bañuelos-Hernández, Miriam Angulo, Edgar Trujillo, Carlos Angulo
Frontiers in Immunology.2025;[Epub] CrossRef - Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
Vaccines.2023; 11(2): 479. CrossRef - Humoral immune response in Asian seabass vaccinated with inactivated and recombinant viral nervous necrosis vaccine
M. Makesh, N. Venkata Satyanarayana, K. Muddukrishnaiah, Sujeet Kumar, G. Thiagarajan, Ashok Kumar Jangam, R. Subburaj, M. Kailasam, K.K. Vijayan
Aquaculture.2023; 569: 739384. CrossRef - Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts
Xiaosong Gu, Lei Huang, Jiazhang Lian
Synthetic and Systems Biotechnology.2023; 8(3): 469. CrossRef - Yeast as carrier for drug delivery and vaccine construction
Yifu Tan, Liwei Chen, Ke Li, Beibei Lou, Yanfei Liu, Zhenbao Liu
Journal of Controlled Release.2022; 346: 358. CrossRef - Construction of Attenuated Strains for Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) via Reverse Genetic System
Yingying Lei, Yu Xiong, Dagang Tao, Tao Wang, Tianlun Chen, Xufei Du, Gang Cao, Jiagang Tu, Jinxia Dai
Viruses.2022; 14(8): 1737. CrossRef - Long-Term Protection and Serologic Response of European Sea Bass Vaccinated with a Betanodavirus Virus-Like Particle Produced in Pichia pastoris
Sofie Barsøe, Anna Toffan, Francesco Pascoli, Ansgar Stratmann, Tobia Pretto, Andrea Marsella, Mériem Er-Rafik, Niccolò Vendramin, Niels J. Olesen, Dagoberto Sepúlveda, Niels Lorenzen
Vaccines.2021; 9(5): 447. CrossRef - Plant-Produced Vaccines: Future Applications in Aquaculture
Hang Su, Igor A. Yakovlev, André van Eerde, Jianguo Su, Jihong Liu Clarke
Frontiers in Plant Science.2021;[Epub] CrossRef - Recombinant Baculovirus-Produced Grass Carp Reovirus Virus-Like Particles as Vaccine Candidate That Provides Protective Immunity against GCRV Genotype II Infection in Grass Carp
Ting Gao, Caixia Gao, Siyu Wu, Yingying Wang, Jiyuan Yin, Yingying Li, Weiwei Zeng, Sven M. Bergmann, Qing Wang
Vaccines.2021; 9(1): 53. CrossRef - Developing oral nanovaccines for fish: a modern trend to fight infectious diseases
Carlos Angulo, Marlene Tello‐Olea, Martha Reyes‐Becerril, Elizabeth Monreal‐Escalante, Luis Hernández‐Adame, Miriam Angulo, José M. Mazon‐Suastegui
Reviews in Aquaculture.2021; 13(3): 1172. CrossRef - Contribution of yeast models to virus research
R Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Applied Microbiology and Biotechnology.2021; 105(12): 4855. CrossRef - Yarrowia lipolytica, health benefits for animals
Francisco A. Guardiola, María Ángeles Esteban, Carlos Angulo
Applied Microbiology and Biotechnology.2021; 105(20): 7577. CrossRef - Betanodavirus and VER Disease: A 30-year Research Review
Isabel Bandín, Sandra Souto
Pathogens.2020; 9(2): 106. CrossRef - Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
Eun Jung Thak, Su Jin Yoo, Hye Yun Moon, Hyun Ah Kang
FEMS Yeast Research.2020;[Epub] CrossRef - Yeast-based vaccines: New perspective in vaccine development and application
Ravinder Kumar, Piyush Kumar
FEMS Yeast Research.2019;[Epub] CrossRef - Development of conditional cell lysis mutants of Saccharomyces cerevisiae as production hosts by modulating OCH1 and CHS3 expression
Van-Trinh Luu, Hye Yun Moon, Su Jin Yoo, Jin Ho Choo, Eun Jung Thak, Hyun Ah Kang
Applied Microbiology and Biotechnology.2019; 103(5): 2277. CrossRef - An effective and rapid method for RNA preparation from non-conventional yeast species
Dong Wook Lee, Chang Pyo Hong, Hyun Ah Kang
Analytical Biochemistry.2019; 586: 113408. CrossRef - A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches
Jie Ma, Timothy J. Bruce, Evan M. Jones, Kenneth D. Cain
Microorganisms.2019; 7(11): 569. CrossRef - Vaccination with UV-inactivated nodavirus partly protects European sea bass against infection, while inducing few changes in immunity
Yulema Valero, Djamal Mokrani, Elena Chaves-Pozo, Marta Arizcun, Mustapha Oumouna, José Meseguer, M.Ángeles Esteban, Alberto Cuesta
Developmental & Comparative Immunology.2018; 86: 171. CrossRef - Cell Surface Display of Red-Grouper Nervous Necrosis Virus Capsid Protein on <i>Pichia pastoris</i>
Uraiwan Intamaso, Palatip Chutoam, Witthaya Poomipak, Nopadon Pirarat
Advances in Microbiology.2018; 08(11): 830. CrossRef
- Cell-Surface Expression of Aspergillus saitoi-Derived Functional α-1,2-Mannosidase on Yarrowia lipolytica for Glycan Remodeling
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Hye Yun Moon , Trinh Luu Van , Seon Ah Cheon , Jinho Choo , Jeong-Yoon Kim , Hyun Ah Kang
-
J. Microbiol. 2013;51(4):506-514. Published online August 30, 2013
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DOI: https://doi.org/10.1007/s12275-013-3344-x
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841
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Abstract
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Expression of proteins on the surface of yeast has a wide range of applications, such as development of live vaccines, screening of antibody libraries, and use as whole-cell biocatalysts. The hemiascomycetes yeast Yarrowia lipolytica has been raised as a potential host for heterologous expression of recombinant proteins. In this study, we report the expression of Aspergillus saitoi α-1,2-mannosidase, encoded by the msdS gene, on the cell surface of Y. lipolytica. As the first step to achieve the secretory expression of msdS protein, four different signal sequences-derived from the endogenous Y. lipolytica Lip2 and Xpr2 prepro regions and the heterologous A. niger α-amylase and rice α-amylase signal sequences-were analyzed for their secretion efficiency. It was shown that the YlLip2 prepro sequence was most efficient in directing the secretory expression of msdS in fully N-glycosylated forms. The surface display of msdS was subsequently directed by fusing GPI anchoring motifs derived from Y. lipolytica cell wall proteins, YlCwp1p and YlYwp1p, respectively, to the C-terminus of the Lip2 prepro-msdS protein. The expression of actively functional msdS protein on the cell surface was confirmed by western blot, flow cytometry analysis, along with the α-1,2-mannosidase activity assay using intact Y. lipolytica cells as the enzyme source. Furthermore, the glycoengineered Y. lipolytica Δoch1Δmpo1 strains displaying α-1,2-mannosidase were able to convert Man8GlcNAc2 to Man5GlcNAc2 efficiently on their cell-wall mannoproteins, demonstrating its potential used for glycoengineering in vitro or in vivo.
-
Citations
Citations to this article as recorded by - Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production
Maria Orfanidou, Eleftheria Panagiotidou, Antonios M. Makris, Eleni Theodosiou
Biotechnology for the Environment.2025;[Epub] CrossRef - Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications
Karla V. Teymennet-Ramírez, Fernando Martínez-Morales, María R. Trejo-Hernández
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Dietary intake of GDF11 delays the onset of several biomarkers of aging in male mice through anti-oxidant system via Smad2/3 pathway
Lili Song, Fei Wu, Congjun Li, Shicui Zhang
Biogerontology.2022; 23(3): 341. CrossRef - Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin
Ewelina Celińska, Jean-Marc Nicaud, Wojciech Białas
Applied Microbiology and Biotechnology.2021; 105(3): 975. CrossRef - Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement
Catherine Madzak
Journal of Fungi.2021; 7(7): 548. CrossRef - Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations
Catherine Madzak
Molecular Biotechnology.2018; 60(8): 621. CrossRef - Synthetic biology tools for engineering Yarrowia lipolytica
M. Larroude, T. Rossignol, J.-M. Nicaud, R. Ledesma-Amaro
Biotechnology Advances.2018; 36(8): 2150. CrossRef - Development of recombinant Yarrowia lipolytica producing virus-like particles of a fish nervous necrosis virus
Van-Trinh Luu, Hye Yun Moon, Jee Youn Hwang, Bo-Kyu Kang, Hyun Ah Kang
Journal of Microbiology.2017; 55(8): 655. CrossRef - Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
Microbial Cell Factories.2017;[Epub] CrossRef -
Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering
Catherine Madzak
Applied Microbiology and Biotechnology.2015; 99(11): 4559. CrossRef - Biotechnological applications of Yarrowia lipolytica: Past, present and future
Hu-Hu Liu, Xiao-Jun Ji, He Huang
Biotechnology Advances.2015; 33(8): 1522. CrossRef - Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica
Jeong-Nam Park, Chang Pyo Han, Dong-Jik Lee, Seon Ah Cheon, Hyun Ah Kang
Journal of Microbiology.2014; 52(4): 284. CrossRef - Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha
Seon Ah Cheon, Jinhee Jung, Jin Ho Choo, Doo-Byoung Oh, Hyun Ah Kang
Biotechnology Letters.2014; 36(10): 2085. CrossRef
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