- Differences in seroprevalence between epicenter and non-epicenter areas of the COVID-19 outbreak in South Korea
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Hye Won Jeong , Hyun-Ha Chang , Eun Ji Kim , Yu Kyung Kim , Se-Mi Kim , Eun-Ha Kim , Young-Il Kim , Mark Anthony B. Casel , Seong-Gyu Kim , Rare Rollon , Seung-Gyu Jang , Kwang-Min Yu , Hee-Sung Kim , Hee Sue Park , Su-Jin Park , Yong-Dae Kim , Eung-Gook Kim , Young Ki Choi
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J. Microbiol. 2021;59(5):530-533. Published online April 28, 2021
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DOI: https://doi.org/10.1007/s12275-021-1095-7
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To compare the standardized severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) seroprevalence of high
epicenter region with non-epicenter region, serological studies
were performed with a total of 3,268 sera from Daegu City
and 3,981 sera from Chungbuk Province. Indirect immunofluorescence
assay (IFA) for SARS-CoV-2 IgG results showed
a high seroprevalence rate in the Daegu City (epicenter) compared
with a non-epicenter area (Chungbuk Province) (1.27%
vs. 0.91%, P = 0.0358). It is noteworthy that the highest seroprevalence
in Daegu City was found in elderly patients (70’s)
whereas young adult patients (20’s) in Chungbuk Province
showed the highest seroprevalence. Neutralizing antibody
(NAb) titers were found in three samples from Daegu City
(3/3, 268, 0.09%) while none of the samples from Chungbuk
Province were NAb positive. These results demonstrated that
even following the large outbreak, the seropositive rate of
SARS-CoV-2 in the general population remained low in
South Korea.
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Citations
Citations to this article as recorded by  - Integrated Clinical and Social Impacts of the COVID-19 Pandemic in Korea: A Combined Systematic and Narrative Review
Yumi Jang, Jaehun Jung, Kyong Ran Peck
Journal of Korean Medical Science.2026;[Epub] CrossRef - Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
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Journal of Korean Medical Science.2022;[Epub] CrossRef
- Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity
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Young-Il Kim , Mark Anthony B. Casel , Se-Mi Kim , Seong-Gyu Kim , Su-Jin Park , Eun-Ha Kim , Hye Won Jeong , Haryoung Poo , Young Ki Choi
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J. Microbiol. 2020;58(10):886-891. Published online September 29, 2020
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DOI: https://doi.org/10.1007/s12275-020-0335-6
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1,104
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2
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28
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29
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Abstract
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Various treatments and agents had been reported to inactivate
RNA viruses. Of these, thermal inactivation is generally
considered an effective and cheap method of sample
preparation for downstream assays. The purpose of this study
is to establish a safe inactivation method for SARS-CoV-2
without compromising the amount of amplifiable viral genome
necessary for clinical diagnoses. In this study, we demonstrate
the infectivity and genomic stability of SARSCoV-
2 by thermal inactivation at both 56°C and 65°C. The
results
substantiate that viable SARS-CoV-2 is readily inactivated
when incubated at 56°C for 30 min or at 65°C for
10 min. qRT-PCR of specimens heat-inactivated at 56°C for
30 min or 65°C for 15 min revealed similar genomic RNA
stability compared with non-heat inactivated specimens. Further,
we demonstrate that 30 min of thermal inactivation at
56°C could inactivate viable viruses from clinical COVID-19
specimens without attenuating the qRT-PCR diagnostic sensitivity.
Heat treatment of clinical specimens from COVID-19
patients at 56°C for 30 min or 65°C for 15 min could be a useful
method
for the inactivation of a highly contagious agent,
SARS-CoV-2. Use of this method would reduce the potential
for secondary infections in BSL2 conditions during diagnostic
procedures. Importantly, infectious virus can be inactivated
in clinical specimens without compromising the
sensitivity of the diagnostic RT-PCR assay.
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