- Genome sequence analysis of potential probiotic strain Leuconostoc lactis EFEL005 isolated from kimchi
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Jin Seok Moon , Hye Sun Choi , So Yeon Shin , Sol Ji Noh , Che Ok Jeon , Nam Soo Han
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J. Microbiol. 2015;53(5):337-342. Published online May 3, 2015
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DOI: https://doi.org/10.1007/s12275-015-5090-8
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Leuconostoc lactis EFEL005 (KACC 91922) isolated from
kimchi showed promising probiotic attributes; resistance
against acid and bile salts, absence of transferable genes for
antibiotic resistance, broad utilization of prebiotics, and no
hemolytic activity. To expand our understanding of the species,
we generated a draft genome sequence of the strain and
analyzed its genomic features related to the aforementioned
probiotic properties. Genome assembly resulted in 35 contigs,
and the draft genome has 1,688,202 base pairs (bp) with
a G+C content of 43.43%, containing 1,644 protein-coding
genes and 50 RNA genes. The average nucleotide identity
analysis showed high homology (≥ 96%) to the type strain
L. lactis KCTC3528, but low homology (≤ 95%) to L. lactis
KCTC3773 (formerly L. argentinum). Genomic analysis revealed
the presence of various genes for sucrose metabolism
(glucansucrases, invertases, sucrose phosphorylases, and
mannitol dehydrogenase), acid tolerance (F1F0 ATPases, cation
transport ATPase, branched-chain amino acid permease,
and lysine decarboxylase), vancomycin response regulator,
and antibacterial peptide (Lactacin F). No gene for production
of biogenic amines (histamine and tyramine) was found.
This report will facilitate the understanding of probiotic
properties of this strain as a starter for fermented foods.
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Citations
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