- Molecular Phylogenetic Status of Korean Strain of Podosphaera xanthii, a Causal Pathogen of Powdery Mildew on Japanese Thistle (Cirsium japonicum) in Korea
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Hyang Burm Lee
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J. Microbiol. 2012;50(6):1075-1080. Published online December 30, 2012
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DOI: https://doi.org/10.1007/s12275-012-2618-z
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Powdery mildew diseases are sensitive to climate change and spread can be favored by increased temperature and low moisture. During 2011 to 2012, a powdery mildew disease by a Podosphaera species was observed on the leaves of Japanese thistle (Cirsium japonicum) in Korea. The initial sign of this disease included scattered superficial white mycelia on leaves. As the disease progressed, abundant necrotic black spots exhibiting chasmothecia were formed on the leaves. rDNA ITS and 28S homologies of the fungus (EML-CSPW1) showed 100% identity values with those regions from many strains of P. xanthii (syn. P. fusca) via NCBI BLASTN search.
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Seunghwan Kim, Myunghee Jung, Eun A. Oh, Tae Ho Kim, Jeong-Gu Kim Mitochondrial DNA Part B.2019; 4(2): 4172. CrossRef - Pectolinarin inhibits proliferation, induces apoptosis, and suppresses inflammation in rheumatoid arthritis fibroblast‐like synoviocytes by inactivating the phosphatidylinositol 3 kinase/protein kinase B pathway
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Seo Hee Lee, Thuong T. T. Nguyen, Hyang Burm Lee Mycobiology.2018; 46(3): 205. CrossRef - Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus
Thuong T. T. Nguyen, Hyang Burm Lee Mycobiology.2018; 46(4): 317. CrossRef - In renal hypertension, Cirsium japonicum strengthens cardiac function via the intermedin/nitric oxide pathway
Xiaoling Yang, Hui Shao, Yong Chen, Ning Ding, Anning Yang, Jue Tian, Yuanxu Jiang, Guizhong Li, Yideng Jiang Biomedicine & Pharmacotherapy.2018; 101: 787. CrossRef - Isolation and Characterization of Three Unrecorded Zygomycete Fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans
Thuong T. T. Nguyen, Young-Joon Choi, Hyang Burm Lee Mycobiology.2017; 45(4): 318. CrossRef - Characterization of Powdery Mildew Caused by Podosphaera fusca Infecting Melothria japonica(Thunb.) Maxim
Jin-Hyeuk Kwon, Hyeong-Jin Jee, Jinwoo Kim Journal of Agriculture & Life Science.2015; 49(5): 47. CrossRef
- Diversity and Physiological Properties of Root Endophytic Actinobacteria in Native Herbaceous Plants of Korea
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Tae-Ui Kim , Sung-Heun Cho , Ji-Hye Han , Young Min Shin , Hyang Burm Lee , Seung Bum Kim
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J. Microbiol. 2012;50(1):50-57. Published online February 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1417-x
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950
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Endophytic actinobacterial diversity in the native herbaceous
plant species of Korea was analyzed using a culturebased
approach. Sixty one actinobacterial strains were isolated,
and assigned to 15 genera based on 16S rRNA gene
analysis. The members of the genus Streptomyces comprised
45.9% of the total isolates, followed by Micromonospora
(18.8%), Rhodococcus (6.6%), Microbispora (4.9%), and
Micrococcus (4.9%). Other minor constituents included
members of Microbacterium, Streptacidiphilus, Arthrobacter,
Dietzia, Kitasatospora, Herbiconiux, Mycobacterium, Nocardia,
Rathayibacter, and Tsukamurella. Among the isolates, 65.6%
exhibited at least one hydrolytic enzyme activity out of four,
and 45.9% exhibited antagonistic activity against at least
one fungal pathogen out of five, thus demonstrating that
endophytic actinobacteria can be an important source of
bioactive compounds. Notably, most strains of Streptomyces
proved active for both enzymatic and antagonistic activities.
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- Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)
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Young Tae Kim , Myoungho Cho , Je Yong Jeong , Hyang Burm Lee , Seung Bum Kim
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J. Microbiol. 2010;48(5):566-572. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0126-6
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690
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Microbial communities in hot pepper (Capsicum annuum L.) cultivation fields under different cultivation methods were investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis. Rhizosphere soil and leaf samples were collected from control, conventional and nature-friendly cultivation fields between May and July, 2009. Two Bacillus subtilis strains were applied to nature-friendly cultivation fields as biocontrol agents during the sampling period. Relative abundances of bacteria and plant pathogenic fungi related T-RFs were also measured to monitor the effect of biocontrol agents on potential plant pathogenic fungi. In the principal component analysis (PCA) based on T-RFLP profiles, the microbial communities from rhizosphere soil samples in July, including bacteria and fungi, showed distinct difference between nature-friendly cultivation fields and other cultivation fields. However, there was no correlation between cultivation methods and leaf microbial communities at any sampling period. Changes in the abundance of bacteria related T-RF in the rhizosphere of nature-friendly cultivation fields were observed clearly two months after application of biocontrol agent, while the abundance of plant pathogenic fungi related T-RFs significantly decreased.
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- Evaluation of Insecticidal Activity of a Bacterial Strain, Serratia sp. EML-SE1 against Diamondback Moth
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Hyung Uk Jeong , Hye Yeon Mun , Hyung Keun Oh , Seung Bum Kim , Kwang Yeol Yang , Iksoo Kim , Hyang Burm Lee
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J. Microbiol. 2010;48(4):541-545. Published online August 20, 2010
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DOI: https://doi.org/10.1007/s12275-010-0221-9
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To identify novel bioinsecticidal agents, a bacterial strain, Serratia sp. EML-SE1, was isolated from a dead larva of the lepidopteran diamondback moth (Plutella xylostella) collected from a cabbage field in Korea. In this study, the insecticidal activity of liquid cultures in Luria-Bertani broth (LBB) and nutrient broth (NB) of a bacterial strain, Serratia sp. EML-SE1 against thirty 3rd and 4th instar larvae of the diamondback moth was investigated on a Chinese cabbage leaf housed in a round plastic cage (Ø 10×6 cm). 72 h after spraying the cabbage leaf with LBB and NB cultures containing the bacterial strain, the mortalities of the larvae were determined to be 91.7% and 88.3%, respectively. In addition, the insecticidal activity on potted cabbage containing 14 leaves in a growth cage (165×83×124 cm) was found to be similar to that of the plastic cage experiment. The results of this study provided valuable information on the insecticidal activity of the liquid culture of a Serratia species against the diamondback moth.
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