Journal of Microbiology

Hui Zhang

3 Articles

The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2: Hui Zhang , Junxi Zheng , Li Yi , Yue Li , Zhe Ma , Hongjie Fan , Chengping Lu; J. Microbiol. 2014;52(11):963-969. Published online October 31, 2014; DOI: https://doi.org/10.1007/s12275-014-4311-x

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Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type І. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type І binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type І binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis that interact with fibronectin or collagen type І.

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Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig
Yanyan Zhang, Rui Li, Qian Li, Yongwei Zhu, Xiaopei Yang, Di Zhao, Bingbing Zong
BMC Veterinary Research.2023;[Epub] CrossRef
Schistosoma mansoni phosphoglycerate mutase: a glycolytic ectoenzyme with thrombolytic potential
David B. Pirovich, Akram A. Da’dara, Patrick J. Skelly
Parasite.2022; 29: 41. CrossRef
Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions
Haodan Zhu, Junming Zhou, Dandan Wang, Zhengyu Yu, Bin Li, Yanxiu Ni, Kongwang He
Archives of Microbiology.2021; 203(7): 4715. CrossRef
Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2
Quan Li, Yang Fu, Genglin Guo, Zhuohao Wang, Wei Zhang
AMB Express.2020;[Epub] CrossRef
Identification of two adhesins of Actinobacillus seminis
J. Fernando Montes-García, Delil A. Chincoya Martinez, Sergio Vaca Pacheco, Candelario Vázquez Cruz, Patricia Sanchez Alonso, Juan Xicohtencatl Cortes, Hector Trujillo-Ruiz, Erasmo Negrete-Abascal
Small Ruminant Research.2018; 167: 100. CrossRef
Characterization and functional analysis of PnuC that is involved in the oxidative stress tolerance and virulence of Streptococcus suis serotype 2
Quan Li, Yuhang Zhang, Du Dechao, Yu Yanfei, Wei Zhang
Veterinary Microbiology.2018; 216: 198. CrossRef
Understanding the virulence of Streptococcus suis: A veterinary, medical, and economic challenge
B. Haas, D. Grenier
Médecine et Maladies Infectieuses.2018; 48(3): 159. CrossRef
Factor H specifically capture novel Factor H-binding proteins of Streptococcus suis and contribute to the virulence of the bacteria
Quan Li, Caifeng Ma, Yang Fu, Yanan He, Yanfei Yu, Dechao Du, Huochun Yao, Chengping Lu, Wei Zhang
Microbiological Research.2017; 196: 17. CrossRef
The surface-displayed chaperones GroEL and DnaK of Mycoplasma pneumoniae interact with human plasminogen and components of the extracellular matrix
Lisa Hagemann, Anne Gründel, Enno Jacobs, Roger Dumke
Pathogens and Disease.2017;[Epub] CrossRef
Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses
Mariela Segura, Cynthia Calzas, Daniel Grenier, Marcelo Gottschalk
FEBS Letters.2016; 590(21): 3772. CrossRef
Interactions of surface-displayed glycolytic enzymes of Mycoplasma pneumoniae with components of the human extracellular matrix
Anne Gründel, Enno Jacobs, Roger Dumke
International Journal of Medical Microbiology.2016; 306(8): 675. CrossRef
Identification of Novel Laminin- and Fibronectin-binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2
Quan Li, Hanze Liu, Dechao Du, Yanfei Yu, Caifeng Ma, Fangfang Jiao, Huochun Yao, Chengping Lu, Wei Zhang
Frontiers in Cellular and Infection Microbiology.2015;[Epub] CrossRef

A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection: Junbo Zhang , Shuanghong Yin , Fei Guo , Ren Meng , Chuangfu Chen , Hui Zhang , Zhiqiang Li , Qiang Fu , Huijun Shi , Shengwei Hu , Wei Ni , Tiansen Li , Ke Zhang; J. Microbiol. 2014;52(8):681-688. Published online July 4, 2014; DOI: https://doi.org/10.1007/s12275-014-3689-9

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Abstract PDF

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

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The Brucella abortus A19ΔfeuPΔfeuQ double-mutant is highly attenuated and confers protection in BALB/c mice
Zhiqiang Li, Shuli Wang, Huijun Zhang, Ruirui Li, Yanyan Cui, Jinliang Zhang, Junfang Hao, Qifeng Li
Vaccine.2026; 76: 128325. CrossRef
A Meta-Analysis of the Protective Efficacy of Brucella abortus S2308 Gene-Deletion Mutant Vaccines Compared to Conventional Vaccines in Mice
Jing Yuan, Maoyuan Gu, Rongrong Ni, Xufeng Liu, Qimin Dong, Mengdi Dong, Shuqi Dong, Zeyao Wang, Tianyue Zhang, Yuting Zhang, Zhujie Fu, Junyuan Li, Yanbing Zhang, Huan Zhang, Zhongchen Ma, Junbo Zhang, Jihai Yi, Yueli Wang
Microorganisms.2026; 14(7): 1419. CrossRef
Disruption of Erythritol Catabolism via the Deletion of Fructose-Bisphosphate Aldolase (Fba) and Transaldolase (Tal) as a Strategy to Improve the Brucella Rev1 Vaccine
Aitor Elizalde-Bielsa, Leticia Lázaro-Antón, María Jesús de Miguel, Pilar M. Muñoz, Raquel Conde-Álvarez, Amaia Zúñiga-Ripa
International Journal of Molecular Sciences.2024; 25(20): 11230. CrossRef
Establishment of picodroplet-based co-culture system to improve erythritol production in Yarrowia lipolytica
Shuang Li, Yuan Zhang, Lu Li, Yaomeng Yuan, Haoxuan Sun, Xin-Hui Xing, Xiaoyan Wang, Chong Zhang
Biochemical Engineering Journal.2023; 198: 109036. CrossRef
Characterization of Brucella abortus Mutant A19mut2, a Potential DIVA Vaccine Candidate with a Modification on Lipopolysaccharide
Hosny Ahmed Abdelgawad, Zhengmin Lian, Yi Yin, Tian Fang, Mingxing Tian, Shengqing Yu
Vaccines.2023; 11(7): 1273. CrossRef
Evaluation of Brucellosis Vaccines: A Comprehensive Review
Mohsen Heidary, Shirin Dashtbin, Roya Ghanavati, Marzie Mahdizade Ari, Narjess Bostanghadiri, Atieh Darbandi, Tahereh Navidifar, Malihe Talebi
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Advances and prospects of transcription‐factor‐based biosensors in high‐throughput screening for cell factories construction
Changfan Li, Chang Wang, Jiang Zhu, Feng Xue, Xiaoman Sun, Yang Gu
Food Bioengineering.2022; 1(2): 135. CrossRef
Development of Brucella melitensis Rev.1 ΔOmp19 mutants with DIVA feature and comparison of their efficacy against three commercial vaccines in a mouse model
Ali Uslu, Osman Erganis
Molecular Immunology.2021; 133: 44. CrossRef
Insights into irr and rirA gene regulation on the virulence of Brucella melitensis M5-90
Huan Zhang, Benben Wang, Wenxing Wu, Xiaoyu Deng, Zhiran Shao, Jihai Yi, Zhen Wang, Ningning Yang, Yong Wang, Yuanzhi Wang, Chuangfu Chen
Canadian Journal of Microbiology.2020; 66(5): 351. CrossRef
Combining genetically-encoded biosensors with high throughput strain screening to maximize erythritol production in Yarrowia lipolytica
Xueliang Qiu, Peng Xu, Xinrui Zhao, Guocheng Du, Juan Zhang, Jianghua Li
Metabolic Engineering.2020; 60: 66. CrossRef
Research progress on the role of immune cells in Brucella infection
Jin Zhang
Infection International.2018; 7(1): 23. CrossRef
Development and evaluation of in murine model, of an improved live-vaccine candidate against brucellosis from to Brucella melitensis vjbR deletion mutant
Zhiqiang Li, Shuli Wang, Hui Zhang, Li Xi, Jinliang Zhang, Xiaogen Zhang, Qingfeng Zhou, Jihai Yi, Min Li, Weihua Zhang, Junbo Zhang
Microbial Pathogenesis.2018; 124: 250. CrossRef
Expression and regulation of the ery operon of Brucella melitensis in human trophoblast cells
Hui Zhang, Xiaoxia Dou, Zhiqiang Li, Yu Zhang, Jing Zhang, Fei Guo, Yuanzhi Wang, Zhen Wang, Tiansen Li, Xinli Gu, Chuangfu Chen
Experimental and Therapeutic Medicine.2016; 12(4): 2723. CrossRef
Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models
Ae Jeong KWON, Ja Young MOON, Won Kyong KIM, Suk KIM, Jin HUR
Journal of Veterinary Medical Science.2016; 78(10): 1541. CrossRef
Meta-Analysis and Advancement of Brucellosis Vaccinology
Tatiane F. Carvalho, João Paulo A. Haddad, Tatiane A. Paixão, Renato L. Santos, Roy Martin Roop
PLOS ONE.2016; 11(11): e0166582. CrossRef
The Brucella melitensis M5-90 phosphoglucomutase (PGM) mutant is attenuated and confers protection against wild-type challenge in BALB/c mice
Yu Zhang, Tiansen Li, Jing Zhang, Zhiqiang Li, Yan Zhang, Zhen Wang, Hanping Feng, Yuanzhi Wang, Chuangfu Chen, Hui Zhang
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef
A Brucella melitensis M5-90 wboA deletion strain is attenuated and enhances vaccine efficacy
Zhi-Qiang Li, Jing-Xue Shi, Wen-Dong Fu, Yu Zhang, Jing Zhang, Zhen Wang, Tian-Sen Li, Chuang-Fu Chen, Fei Guo, Hui Zhang
Molecular Immunology.2015; 66(2): 276. CrossRef

Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells: Qiang Fu , Huijun Shi , Yan Ren , Fei Guo , Wei Ni , Jun Qiao , Pengyan Wang , Hui Zhang , Chuangfu Chen; J. Microbiol. 2014;52(7):619-625. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3479-4

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Abstract PDF

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

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