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Hua Yang 2 Articles
Prevalence and characteristics of the mcr-1 gene in retail meat samples in Zhejiang Province, China
Biao Tang , Jiang Chang , Yi Luo , Han Jiang , Canying Liu , Xingning Xiao , Xiaofeng Ji , Hua Yang
J. Microbiol. 2022;60(6):610-619.   Published online March 31, 2022
DOI: https://doi.org/10.1007/s12275-022-1597-y
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  • 4 Download
  • 23 Web of Science
  • 20 Crossref
AbstractAbstract PDF
Considering the serious threat to food safety and public health posed by pathogens with colistin resistance, colistin was banned as a growth promoter in 2017 in China. In recent years, the resistance rate of Escherichia coli isolated from animal intestines or feces to colistin has decreased. However, the prevalence and characteristics of the mcr-1 gene in retail meat have not been well explored. Herein, 106 mcr-1-negative and 16 mcr- 1-positive E. coli isolates were randomly recovered from 120 retail meat samples and screened using colistin. The 106 E. coli isolates showed maximum resistance to sulfafurazole (73.58%) and tetracycline (62.26%) but susceptibility to colistin (0.00%). All 16 mcr-1-positive E. coli isolates showed resistance to colistin, were extended spectrum beta-lactamase (ESBL)-positive and exhibited complex multidrug resistance (MDR). For these 16 isolates, 17 plasmid replicons and 42 antibiotic resistance genes were identified, and at least 7 antibiotic resistance genes were found in each isolate. Acquired disinfectant resistance genes were identified in 75.00% (12/16) of the isolates. Furthermore, comparative genomic and phylogenetic analysis
results
indicated that these 16 mcr-1-positive E. coli isolates and the most prevalent mcr-1-harboring IncI2 plasmid in this study were closely related to other previously reported mcr-1-positive E. coli isolates and the IncI2 plasmid, respectively, showing their wide distribution. Taken together, our findings showed that retail meat products were a crucial reservoir of mcr-1 during the colistin ban period and should be continuously monitored.

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Citations to this article as recorded by  
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  • Co-occurrence of mcr-1.1, mcr-3.40, and tmexCD1-toprJ1 on a hybrid IncFIB/IncHI1B plasmid in Klebsiella pneumoniae from pork in Hainan, China
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  • Characteristics of Escherichia coli co-harboring blaNDM and mcr-1 genes from market chicken meat
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  • Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital
    Chenlu Xiao, Xuming Li, Lianjiang Huang, Huiluo Cao, Lizhong Han, Yuxing Ni, Han Xia, Zhitao Yang
    Frontiers in Cellular and Infection Microbiology.2023;[Epub]     CrossRef
  • Farm to table: colistin resistance hitchhiking through food
    Absar Talat, Carla Miranda, Patrícia Poeta, Asad U. Khan
    Archives of Microbiology.2023;[Epub]     CrossRef
  • Detection of mcr-1-harbouring Escherichia coli by quantum dot labelling of synthetic small peptides mimicking lipopolysaccharide receptors
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    International Journal of Antimicrobial Agents.2023; 62(3): 106898.     CrossRef
  • Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China
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  • Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China
    Wei Zhou, Rumeng Lin, Zhijin Zhou, Jiangang Ma, Hui Lin, Xue Zheng, Jingge Wang, Jing Wu, Yuzhi Dong, Han Jiang, Hua Yang, Zhangnv Yang, Biao Tang, Min Yue
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  • The Occurrence and Genomic Characteristics of mcr-1-Harboring Salmonella from Retail Meats and Eggs in Qingdao, China
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Core gut microbiota in Jinhua pigs and its correlation with strain, farm and weaning age
Hua Yang , Yingping Xiao , Junjun Wang , Yun Xiang , Yujie Gong , Xueting Wen , Defa Li
J. Microbiol. 2018;56(5):346-355.   Published online May 2, 2018
DOI: https://doi.org/10.1007/s12275-018-7486-8
  • 1,276 View
  • 0 Download
  • 63 Crossref
AbstractAbstract PDF
Gut microbial diversity and the core microbiota of the Jinhua pig, which is a traditional, slow-growing Chinese breed with a high body-fat content, were examined from a total of 105 fecal samples collected from 6 groups of pigs at 3 weaning ages that originated from 2 strains and were raised on 3 different pig farms. The bacterial community was analyzed following high-throughput pyrosequencing of 16S rRNA genes, and the fecal concentrations of short-chain fatty acids (SCFAs) were measured by gas chromatograph. Our results showed that Firmicutes and Bacteroidetes were the dominant phyla, and Lactobacillus, Streptococcus, Clostridium, SMB53, and Bifidobacterium were the most abundant genera. Fifteen predominant genera present in every Jinhua pig sample constituted a phylogenetic core microbiota and included the probiotics Lactobacillus and Bifidobacterium, and the SCFAproducing bacteria Clostridium, Prevotella, Bacteroides, Coprococcus, Roseburia, Ruminococcus, Blautia, and Butyricicoccus. Comparisons of the microbiota compositions and SCFA concentrations across the 6 groups of pigs demonstrated that genetic background and weaning age affected the structure of the gut microbiota more significantly than the farm. The relative abundance of the core genera in the pigs, including Lactobacillus, Clostridium, Prevotella, Bacteroides, Roseburia, Ruminococcus, Blautia, and Butyricicoccus varied dramatically in pigs among the 2 origins and 3 weaning ages, while Oscillospira, Megasphaera, Parabacteroides, and Corynebacterium differed among pigs from different farms. Interestingly, there was a more significant influence of strain and weaning age than of rearing farm on the SCFA concentrations. Therefore, strain and weaning age appear to be the more important factors shaping the intestinal microbiome of pigs.

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