- Accumulation of Lipid Production in Chlorella minutissima by Triacylglycerol Biosynthesis-Related Genes Cloned from Saccharomyces cerevisiae and Yarrowia lipolytica
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Hsin-Ju Hsieh , Chia-Hung Su , Liang-Jung Chien
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J. Microbiol. 2012;50(4):716-716.
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Abstract
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In the article by Hsie et al. that appears in the Journal of Microbiology 2012; 50, 526-534. Page 526, The corresponding author for the article should be Liang-Jung Chien, Graduate School of Biochemical Engineering, Ming Chi University of Technology; 84 Gungjuan Rd., New Taipei 24301, Taiwan (ljchien@mail.mcut.edu.tw).
- Accumulation of Lipid Production in Chlorella minutissima by Triacylglycerol Biosynthesis-Related Genes Cloned from Saccharomyces cerevisiae and Yarrowia lipolytica
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Hsin-Ju Hsieh , Chia-Hung Su , Liang-Jung Chien
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J. Microbiol. 2012;50(3):526-534. Published online June 30, 2012
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DOI: https://doi.org/10.1007/s12275-012-2041-5
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Abstract
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Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity.
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Prachi Nawkarkar, Vikas U. Kapase, Sarika Chaudhary, Sachin Kajla, Shashi Kumar GCB Bioenergy.2023; 15(10): 1240. CrossRef - Expression profile of genes associated with the accumulation of triacylglycerol (TAG) in Auxenochlorella protothecoides KP7 under alkaline pH and nitrogen starvation conditions
Enver Ersoy Andeden, Sahlan Ozturk, Belma Aslim Biocatalysis and Agricultural Biotechnology.2023; 48: 102628. CrossRef - Recent breakthroughs in integrated biomolecular and biotechnological approaches for enhanced lipid and carotenoid production from microalgae
Ashfaq Ahmad, Fawzi Banat, Habiba Alsafar, Shadi W. Hasan Phytochemistry Reviews.2023; 22(4): 993. CrossRef - Microalgae: A promising source for biofuel production
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Anjani Devi Chintagunta, N. S. Sampath Kumar, P. Naveen Kumar, P. Senthil Kumar, S.P. Jeevan Kumar SSRN Electronic Journal.2022;[Epub] CrossRef - Enhancing microalgal lipid accumulation for biofuel production
Zhi Zhu, Jing Sun, Yun Fa, Xufeng Liu, Peter Lindblad Frontiers in Microbiology.2022;[Epub] CrossRef - A review on unit operations, challenges, opportunities, and strategies to improve algal based biodiesel and biorefinery
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Ayesha Shahid, Abd ur Rehman, Muhammad Usman, Muhammad Umer Farooq Ashraf, Muhammad Rizwan Javed, Aqib Zafar Khan, Saba Shahid Gill, Muhammad Aamer Mehmood Biotechnology and Applied Biochemistry.2020; 67(1): 41. CrossRef - Biodiesel from oleaginous microbes: opportunities and challenges
Lohit K. S. Gujjala, S. P. Jeevan Kumar, Bitasta Talukdar, Archana Dash, Sanjeev Kumar, Knawang Ch. Sherpa, Rintu Banerjee Biofuels.2019; 10(1): 45. CrossRef - Improving lipid production by strain development in microalgae: Strategies, challenges and perspectives
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Xiao-Man Sun, Lu-Jing Ren, Quan-Yu Zhao, Xiao-Jun Ji, He Huang Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2019; 1864(4): 552. CrossRef - Biomass and lipid induction strategies in microalgae for biofuel production and other applications
Hossein Alishah Aratboni, Nahid Rafiei, Raul Garcia-Granados, Abbas Alemzadeh, José Rubén Morones-Ramírez Microbial Cell Factories.2019;[Epub] CrossRef - Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans
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Lenny de Jaeger, Benoit M. Carreres, Jan Springer, Peter J. Schaap, Gerrit Eggink, Vitor A. P. Martins Dos Santos, Rene H. Wijffels, Dirk E. Martens, Rafael Vazquez-Duhalt PLOS ONE.2018; 13(4): e0194834. CrossRef - Tailoring Microalgae for Efficient Biofuel Production
Prabin Kumar Sharma, Manalisha Saharia, Richa Srivstava, Sanjeev Kumar, Lingaraj Sahoo Frontiers in Marine Science.2018;[Epub] CrossRef - Algal Green Energy – R&D and technological perspectives for biodiesel production
Rajesh P. Rastogi, Ashok Pandey, Christian Larroche, Datta Madamwar Renewable and Sustainable Energy Reviews.2018; 82: 2946. CrossRef - Innovations in improving lipid production: Algal chemical genetics
Nishikant Wase, Paul Black, Concetta DiRusso Progress in Lipid Research.2018; 71: 101. CrossRef - The role of diatom glucose-6-phosphate dehydrogenase on lipogenic NADPH supply in green microalgae through plastidial oxidative pentose phosphate pathway
Jiao Xue, Ting-Ting Chen, Jian-Wei Zheng, Srinivasan Balamurugan, Jia-Xi Cai, Yu-Hong Liu, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li Applied Microbiology and Biotechnology.2018; 102(24): 10803. CrossRef - Trends and strategies to enhance triacylglycerols and high-value compounds in microalgae
Lisa M. Schüler, Peter S.C. Schulze, Hugo Pereira, Luísa Barreira, Rosa León, João Varela Algal Research.2017; 25: 263. CrossRef - Implications of glycerol metabolism for lipid production
Lu-Lu Xue, Hao-Hong Chen, Jian-Guo Jiang Progress in Lipid Research.2017; 68: 12. CrossRef - Increased fatty acid accumulation following overexpression of glycerol‐3‐phosphate dehydrogenase and suppression of β‐oxidation in oleaginous fungus Mortierella alpina
Hua Yang, Haiqin Chen, Guangfei Hao, Tiantian Mei, Hao Zhang, Wei Chen, Yong Q. Chen European Journal of Lipid Science and Technology.2017;[Epub] CrossRef - Molecular challenges in microalgae towards cost-effective production of quality biodiesel
Young-Soo Chung, Jin-Woo Lee, Chung-Han Chung Renewable and Sustainable Energy Reviews.2017; 74: 139. CrossRef - Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae
Yahaira J. Tamayo‐Ordóñez, Benjamin A. Ayil‐Gutiérrez, Felipe L. Sánchez‐Teyer, Erika A. De la Cruz‐Arguijo, Francisco A. Tamayo‐Ordóñez, Atl Victor Córdova‐Quiroz, Maria C. Tamayo‐Ordóñez Phycological Research.2017; 65(1): 14. CrossRef - Heterologous expression of DGAT genes in the marine microalga Tetraselmis chui leads to an increase in TAG content
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Freddy Guihéneuf, Asif Khan, Lam-Son P. Tran Frontiers in Plant Science.2016;[Epub] CrossRef - dEMBF: A Comprehensive Database of Enzymes of Microalgal Biofuel Feedstock
Namrata Misra, Prasanna Kumar Panda, Bikram Kumar Parida, Barada Kanta Mishra, Shihui Yang PLOS ONE.2016; 11(1): e0146158. CrossRef - Trends and novel strategies for enhancing lipid accumulation and quality in microalgae
Poonam Singh, Sheena Kumari, Abhishek Guldhe, Rohit Misra, Ismail Rawat, Faizal Bux Renewable and Sustainable Energy Reviews.2016; 55: 1. CrossRef - Chlorella species as hosts for genetic engineering and expression of heterologous proteins: Progress, challenge and perspective
Bo Yang, Jin Liu, Yue Jiang, Feng Chen Biotechnology Journal.2016; 11(10): 1244. CrossRef - Current advances in molecular, biochemical, and computational modeling analysis of microalgal triacylglycerol biosynthesis
Sangram K. Lenka, Nicole Carbonaro, Rudolph Park, Stephen M. Miller, Ian Thorpe, Yantao Li Biotechnology Advances.2016; 34(5): 1046. CrossRef - Way forward to achieve sustainable and cost-effective biofuel production from microalgae: a review
N. Misra, P. K. Panda, B. K. Parida, B. K. Mishra International Journal of Environmental Science and Technology.2016; 13(11): 2735. CrossRef - Site-Directed Mutagenesis from Arg195 to His of a Microalgal Putatively Chloroplastidial Glycerol-3-Phosphate Acyltransferase Causes an Increase in Phospholipid Levels in Yeast
Long-Ling Ouyang, Hui Li, Xiao-Jun Yan, Ji-Lin Xu, Zhi-Gang Zhou Frontiers in Plant Science.2016;[Epub] CrossRef - Stable nuclear transformation of the industrial alga Chlorella pyrenoidosa
Conglin Run, Lei Fang, Jianhua Fan, Chengming Fan, Yuanchan Luo, Zanmin Hu, Yuanguang Li Algal Research.2016; 17: 196. CrossRef - In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity
Javier A. Gimpel, Vitalia Henríquez, Stephen P. Mayfield Frontiers in Microbiology.2015;[Epub] CrossRef - Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels
Helge Janßen, Alexander Steinbüchel Biotechnology for Biofuels.2014; 7(1): 7. CrossRef - Edible oils from microalgae: insights in TAG accumulation
A.J. Klok, P.P. Lamers, D.E. Martens, R.B. Draaisma, R.H. Wijffels Trends in Biotechnology.2014; 32(10): 521. CrossRef - Microalgal lipids biochemistry and biotechnological perspectives
Stamatia Bellou, Mohammed N. Baeshen, Ahmed M. Elazzazy, Dimitra Aggeli, Fotoon Sayegh, George Aggelis Biotechnology Advances.2014; 32(8): 1476. CrossRef - Nitrogen Source Affects Glycolipid Production and Lipid Accumulation in the Phytopathogen Fungus <i>Ustilago maydis</i>
Ariana Zavala-Moreno, Roberto Arreguin-Espinosa, Juan Pablo Pardo, Lucero Romero-Aguilar, Guadalupe Guerra-Sánchez Advances in Microbiology.2014; 04(13): 934. CrossRef - In Search of Actionable Targets for Agrigenomics and Microalgal Biofuel Production: Sequence-Structural Diversity Studies on Algal and Higher Plants with a Focus on GPAT Protein
Namrata Misra, Prasanna Kumar Panda OMICS: A Journal of Integrative Biology.2013; 17(4): 173. CrossRef - Biological Challenges and Research Trends in Algae Biofuels
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