- Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
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Yongchao Guan , Meng Zhang , Yingda Wang , Zhongzhuo Liu , Zelin Zhao , Hong Wang , Dingjie An , Aidong Qian , Yuanhuan Kang , Wuwen Sun , Xiaofeng Shan
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J. Microbiol. 2022;60(12):1153-1161. Published online November 10, 2022
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DOI: https://doi.org/10.1007/s12275-022-2373-8
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Aeromonas veronii is a pathogen which can induce diseases in
humans, animals and aquatic organisms, but its pathogenic
mechanism and virulence factors are still elusive. In this study,
we successfully constructed a mutant strain (ΔascP) by homologous
recombination. The results showed that the deletion
of the ascP gene significantly down-regulated the expression
of associated effector proteins in A. veronii compared
to its wild type. The adhesive and invasive abilities of ΔascP to
EPC cells were 0.82-fold lower in contrast to the wild strain.
The toxicity of ΔascP to cells was decreased by about 2.91-fold
(1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant
strain of crucian carp was reduced by 19.94-fold, and
the virulence was considerably attenuated. In contrast to the
wild strain, the ΔascP content in the liver and spleen was considerably
lower. The titers of serum cytokines (IL-8, TNF-α,
and IL-1β) in crucian carp after the infection of the ΔascP strain
were considerably lower in contrast to the wild strain. Hence,
the ascP gene is essential for the etiopathogenesis of A. veronii
TH0426.
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Citations
Citations to this article as recorded by  - Complete genome sequence and genome-wide transposon mutagenesis enable the determination of genes required for sodium hypochlorite tolerance and drug resistance in pathogen Aeromonas veronii GD2019
Yifan Bu, Chengyu Liu, Yabo Liu, Wensong Yu, Tingjin Lv, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
Microbiological Research.2024; 284: 127731. CrossRef - Construction of the flagellin F mutant of Vibrio parahaemolyticus and its toxic effects on silver pomfret (Pampus argenteus) cells
Yang Li, Chao Liu, Yuechen Sun, Ruijun Wang, Choufei Wu, Hanqu Zhao, Liqin Zhang, Dawei Song, Quanxin Gao
International Journal of Biological Macromolecules.2024; 259: 129395. CrossRef - Ferric uptake regulator (fur) affects the pathogenicity of Aeromonas veronii TH0426 by regulating flagellar assembly and biofilm formation
Jin-shuo Gong, Ying-da Wang, Yan-long Jiang, Di Zhang, Ya-nan Cai, Xiao-feng Shan, He Gong, Hao Dong
Aquaculture.2024; 580: 740361. CrossRef
- Discovery of novel glycoside hydrolases from C-glycoside-degrading bacteria using sequence similarity network analysis
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Bin Wei , Ya-Kun Wang , Jin-Biao Yu , Si-Jia Wang , Yan-Lei Yu , Xue-Wei Xu , Hong Wang
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J. Microbiol. 2021;59(10):931-940. Published online September 23, 2021
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DOI: https://doi.org/10.1007/s12275-021-1292-4
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352
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2
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C-Glycosides are an important type of natural product with
significant bioactivities, and the C-glycosidic bonds of C-glycosides
can be cleaved by several intestinal bacteria, as exemplified
by the human faeces-derived puerarin-degrading bacterium
Dorea strain PUE. However, glycoside hydrolases in
these bacteria, which may be involved in the C-glycosidic bond
cleavage of C-glycosides, remain largely unknown. In this
study, the genomes of the closest phylogenetic neighbours of
five puerarin-degrading intestinal bacteria (including Dorea
strain PUE) were retrieved, and the protein-coding genes in
the genomes were subjected to sequence similarity network
(SSN) analysis. Only four clusters of genes were annotated as
glycoside hydrolases and observed in the genome of D. longicatena
DSM 13814T (the closest phylogenetic neighbour of
Dorea strain PUE); therefore, genes from D. longicatena DSM
13814T belonging to these clusters were selected to overexpress
recombinant proteins (CG1, CG2, CG3, and CG4) in
Escherichia coli BL21(DE3). In vitro assays indicated that
CG4 efficiently cleaved the O-glycosidic bond of daidzin and
showed moderate β-D-glucosidase and β-D-xylosidase activity.
CG2 showed weak activity in hydrolyzing daidzin and pNP-
β-D-fucopyranoside, while CG3 was identified as a highly
selective and efficient α-glycosidase. Interestingly, CG3 and
CG4 could be selectively inhibited by daidzein, explaining
their different performance in kinetic studies. Molecular docking
studies predicted the molecular determinants of CG2,
CG3, and CG4 in substrate selectivity and inhibition propensity.
The present study identified three novel and distinctive
glycoside hydrolases, highlighting the potential of SSN
in the discovery of novel enzymes from genomic data.
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Citations
Citations to this article as recorded by - Two-step computational redesign of Bacillus subtilis cellulase and β-glucanase for enhanced thermostability and activity
Huan Zhang, Tong Zhu, Qinglin Zhai, Qiansi Chen, Xuanshuo Zhang, Yiqiang Chen, Wei He, Jingjing Li, Jianqiang Fan, Jiemeng Tao, Xingchuan Hu, Lingfeng Qi, Chaochao Wang, Kuanqi Liao, Yanchun Chen, Yinglu Cui, Shanyi Chen, Bian Wu
International Journal of Biological Macromolecules.2025; 285: 138274. CrossRef - Conserved Local Structural Motifs in Glycoside Hydrolase Families Facilitate the Discovery of Functional Enzymes
Yu-Peng Liang, Ya-Lan Zhao, Zhong-Wei Yin, Xiao-Wei Gong, Xiu-Lin Han, Meng-Liang Wen
Journal of Agricultural and Food Chemistry.2025; 73(19): 11983. CrossRef - A newly isolated human intestinal strain deglycosylating flavonoid C-glycosides
Sha Wang, Siqi Liu, Jing Wang, Jiayue Tao, Mengjiao Wu, Wenfu Ma, Rufeng Wang
Archives of Microbiology.2022;[Epub] CrossRef
- Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling
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Ling Wang , Xuemei Zhang , Guangying Wu , Yuhong Qi , Jinghui Zhang , Jing Yang , Hong Wang , Wenchun Xu
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J. Microbiol. 2020;58(4):330-339. Published online February 27, 2020
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DOI: https://doi.org/10.1007/s12275-020-9538-0
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336
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11
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Streptococcus pneumoniae is a Gram-positive pathogen with
high morbidity and mortality globally but some of its pathogenesis
remains unknown. Previous research has provided
evidence that aminopeptidase N (PepN) is most likely a virulence
factor of S. pneumoniae. However, its role in S. pneumoniae
virulence and its interaction with the host remains
to be confirmed. We generated a pepN gene deficient mutant
strain and found that its virulence for mice was significantly
attenuated as were in vitro adhesion and invasion of host
cells. The PepN protein could induce a strong innate immune
response in vivo and in vitro and induced secretion of IL-6
and TNF-α by primary peritoneal macrophages via the rapid
phosphorylation of MAPK and PI3K/AKT signaling pathways
and this was confirmed using specific pathway inhibitors.
In conclusion, PepN is a novel virulence factor that is
essential for the virulence of S. pneumoniae and induces host
innate immunity via MAPK and PI3K/AKT signaling.
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Citations
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Mingzhu Zhou, Yan Liu, Xin Yin, Jiannan Gong, Jianqiang Li
Discover Oncology.2025;[Epub] CrossRef - Integrative network pharmacology and experimental validation of multi-target synergy against multidrug-resistant klebsiella pneumoniae via PI3K/AKT-MAPK pathway disruption
Tian Man, Huiyong Yu, Lei Li, Weicheng Nie, Chengxiang Wang
Naunyn-Schmiedeberg's Archives of Pharmacology.2025;[Epub] CrossRef - Maternal immune activation mediated prenatal chronic stress induces Th17/Treg cell imbalance may relate to the PI3K/Akt/NF-κB signaling pathway in offspring rats
Ye Li, Guixiang Yao, Rui Wang, Jiashu Zhu, Hongyu Li, Deguang Yang, Shuqin Ma, Youjuan Fu, Can Liu, Suzhen Guan
International Immunopharmacology.2024; 126: 111308. CrossRef - Secreted protein NFA47630 from Nocardia farcinica IFM10152 induces immunoprotective effects in mice
Lichao Han, Xingzhao Ji, Shihong Fan, Jirao Shen, Bin Liang, Zhenjun Li
Tropical Diseases, Travel Medicine and Vaccines.2024;[Epub] CrossRef - Human microbiomes in cancer development and therapy
Chenglai Xia, Jiyan Su, Can Liu, Zhikai Mai, Shuanghong Yin, Chuansheng Yang, Liwu Fu
MedComm.2023;[Epub] CrossRef - Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis
Yanan He, Jixin Li, Yanjun Qu, Liyuan Sun, Xibo Zhao, Han Wu, Guangmei Zhang, Amar Singh
Journal of Immunology Research.2023; 2023: 1. CrossRef - The identification of two M20B family peptidases required for full virulence in Staphylococcus aureus
Nathanial J. Torres, Devon N. Rizzo, Maria A. Reinberg, Mary-Elizabeth Jobson, Brendan C. Totzke, Jessica K. Jackson, Wenqi Yu, Lindsey N. Shaw
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef - Exploration of immune response mechanisms in cadmium and copper co-exposed juvenile golden cuttlefish (Sepia esculenta) based on transcriptome profiling
Xiaokai Bao, Weijun Wang, Xipan Chen, Yanwei Feng, Xiaohui Xu, Guohua Sun, Bin Li, Xiumei Liu, Zan Li, Jianmin Yang
Frontiers in Immunology.2022;[Epub] CrossRef - Pathogenicity and virulence ofStreptococcus pneumoniae: Cutting to the chase on proteases
Mary E. Marquart
Virulence.2021; 12(1): 766. CrossRef - Gut-Lung Microbiota in Chronic Pulmonary Diseases: Evolution, Pathogenesis, and Therapeutics
Chang Yi Shi, Chen Huan Yu, Wen Ying Yu, Hua Zhong Ying, Hua Zhang
Canadian Journal of Infectious Diseases and Medical Microbiology.2021; 2021: 1. CrossRef
- Screening and Identification of ClpE Interaction Proteins in Streptococcus pneumoniae by a Bacterial Two-Hybrid System and Co-immunoprecipitation
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WenJuan Yan , YingYing Cai , Qun Zhang , YuSi Liu , WenChun Xu , YiBing Yin , YuJuan He , Hong Wang , XueMei Zhang
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J. Microbiol. 2013;51(4):453-460. Published online August 30, 2013
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DOI: https://doi.org/10.1007/s12275-013-3001-4
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277
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4
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Hsp100/Clp proteins have crucial functions in the protein quality control, stress tolerance, and virulence of many pathogenic bacteria. ClpE is an important virulence factor involved in adherence and invasion in Streptococcus pneumoniae. To explore the underlying mechanism, we screened ClpE interaction proteins using a bacterial two-hybrid system and co-immunoprecipitation. We used ClpE as bait and constructed the pBT-ClpE bait plasmid for two-hybrid screening. Then, we constructed ClpE::GFP fusion for co-immunoprecipitation screening using anti-GFP monoclonal antibody. We obtained eight potential ClpE interaction proteins, including carbamoyl-phosphate synthase, pyruvate oxidase (SpxB), phosphoenolpyruvate-protein phosphotransferase, aminopeptidase N (pepN), L-lactate dehydrogenase, ribosomal protein S4, sensor histidine kinase (SPD_2019), and FtsW (a cell division protein). FtsW, SpxB, pepN, and SPD_2019 were confirmed to interact with ClpE using Bacterial Two-hybrid or Co-immunoprecipitation. Morphologic observations found that ΔclpE strain existed in abnormal division. β-Galactosidase activity assay suggested that ClpE contributed to the degradation of FtsW. Furthermore, FtsW could be induced by heat shock. The results suggested that ClpE might affect cell division by regulating the level of FtsW. These data may provide new insights in studying the role of ClpE in S. pneumoniae.
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Citations
Citations to this article as recorded by - Legume nodulation and nitrogen fixation require interaction of DnaJ-like protein and lipid transfer protein
Dasong Chen, Dongzhi Li, Ziqi Li, Yuting Song, Qingsong Li, Lihong Wang, Donglai Zhou, Fuli Xie, Youguo Li
Plant Physiology.2023; 193(3): 2164. CrossRef - Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling
Ling Wang, Xuemei Zhang, Guangying Wu, Yuhong Qi, Jinghui Zhang, Jing Yang, Hong Wang, Wenchun Xu
Journal of Microbiology.2020; 58(4): 330. CrossRef - IcmF and DotU are required for the virulence of Acidovorax oryzae strain RS-1
Bin Li, Xiaoxuan Wang, Jie Chen, He Liu, Khattak Arif Ali, Yanli Wang, Wen Qiu, Guochang Sun
Archives of Microbiology.2018; 200(6): 897. CrossRef - Antibacterial effects of Traditional Chinese Medicine monomers against Streptococcus pneumoniae via inhibiting pneumococcal histidine kinase (VicK)
Shuai Zhang, Jianmin Wang, Wenchun Xu, Yusi Liu, Wei Wang, Kaifeng Wu, Zhe Wang, Xuemei Zhang
Frontiers in Microbiology.2015;[Epub] CrossRef
- SP0454, A Putative Threonine Dehydratase, Is Required For Pneumococcal Virulence In Mice
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WenJuan Yan , Hong Wang , WenChun Xu , KaiFeng Wu , Run Yao , XiuYu Xu , Jie Dong , YanQing Zhang , Wen Zhong , XueMei Zhang
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J. Microbiol. 2012;50(3):511-517. Published online June 30, 2012
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DOI: https://doi.org/10.1007/s12275-012-2014-8
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255
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5
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Increasing pressure in antibiotic resistance and the requirement for the design of new vaccines are the objectives of clarifying the putative virulence factors in pneumococcal infection. In this study, the putative threonine dehydratase sp0454 was inactivated by erythromycin-resistance cassette replacement in Streptococcus pneumoniae CMCC 31203 strain. The sp0454 mutant was tested for cell growth, adherence, colonization, and virulence in a murine model. The Δsp0454 mutant showed decreased ability for colonization and impaired ability to adhere to A549 cells. However, the SP0454 polypeptide or its antiserum did not affect pneumococcal CMCC 31203 adhesion to A549 cells. The sp0454 deletion mutant was less virulent in a murine intranasal infection model. Real-time RT-PCR analysis revealed significant decrease of the pneumococcal surface antigen A expression in the sp0454 mutant. These results suggest that SP0454 contributes to virulence and colonization, which could be explained in part by modulating the expression of other virulence factors, such as psaA in pneumococcal infection.
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Citations
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Pratima Chapagain, Ali Ali, Mohamed Salem
Pathogens.2023; 12(3): 436. CrossRef - Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Journal of Microbiology.2017; 55(10): 823. CrossRef - Mucosal Immunization with the Live Attenuated Vaccine SPY1 Induces Humoral and Th2-Th17-Regulatory T Cell Cellular Immunity and Protects against Pneumococcal Infection
Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef - A Novel Protein, RafX, Is Important for Common Cell Wall Polysaccharide Biosynthesis in Streptococcus pneumoniae: Implications for Bacterial Virulence
Kaifeng Wu, Jian Huang, Yanqing Zhang, Wenchun Xu, Hongmei Xu, Libin Wang, Ju Cao, Xuemei Zhang, Yibing Yin
Journal of Bacteriology.2014; 196(18): 3324. CrossRef - Serotype-independent protection against pneumococcal infections elicited by intranasal immunization with ethanol-killed pneumococcal strain, SPY1
Xiuyu Xu, Jiangping Meng, Yiping Wang, Jie Zheng, Kaifeng Wu, Xuemei Zhang, Yibing Yin, Qun Zhang
Journal of Microbiology.2014; 52(4): 315. CrossRef
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