Journal of Microbiology

Hong Ki Min

1 Article

Application of Bovine Viral Diarrhoea Virus as an Internal Control in Nucleic Acid Amplification Tests for Hepatitis C Virus RNA in Plasma-Derived Products: Si Hyung Yoo , Seung Hee Hong , Sa Rah Jung , Su Jin Park , Nam Kyung Lee , Soon Nam Kim , Sang Mo Kang , Hong Ki Min , Sue Nie Park , Seung Hwa Hong; J. Microbiol. 2006;44(1):72-76.; DOI: https://doi.org/2334 [pii]

Abstract PDF: Plasma-derived products are produced from plasma via fractionation and chromatography techniques, but can also be produced by other methods. In the performance of nucleic acid amplification tests (NAT) with plasma-derived products, it is necessary to include an internal control for the monitoring of all procedures. In order to avoid false negative results, we confirmed the usefulness of the bovine viral diarrhoea virus (BVDV) for use as an internal control in the detection of hepatitis C virus (HCV) RNA in plasma-derived products. These products, which were spiked with BVDV, were extracted and then NAT was performed. Specificity and sensitivity were determined via the adjustment of primer concentrations and annealing temperatures. BVDV detection allows for validation in the extraction, reverse transcription, and amplification techniques used for HCV detection in plasma-derived products.

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