- Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica
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Lan Ji Huang , Jinghua Cui , Hong Hua Piao , Yeongjin Hong , Hyon E. Choy , Phil Youl Ryu
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J. Microbiol. 2010;48(5):663-667. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-9268-9
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Cytolysin A (ClyA) is a pore-forming hemolytic protein encoded by the clyA gene. It has been identified in Salmonella enterica serovars Typhi and Paratyphi A. To identify and characterize the clyA genes in various Salmonella enterica strains, 21 different serotypes of strains isolated from clinical specimens were presently examined. Full-length clyA genes were found in S. enterica serovar Brandenburg, Indiana, Panama, and Schwarzengrund strains by polymerase chain reaction amplification. The ClyA proteins from these four strains showed >97% amino acid identity to that of S. enterica serovar Typhi. Although all four serovars expressed detectable levels of ClyA as determined by Western blot analysis, they did not show a strong hemolytic effect on blood agar, indicating that ClyA may not be efficiently expressed or secreted. Escherichia coli transformed with clyA genes from the four serovars enhanced production of ClyA proteins and hemolytic activities to a level similar to S. enterica serovar Typhi ClyA. The present results suggest that ClyA may play a role in the pathogenesis of S. enterica serovar Brandenburg, Indiana, Panama and Schwarzengrund.
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Citations
Citations to this article as recorded by  - Plasmid profile and role in virulence of salmonella enterica serovars isolated from food animals and humans in Lagos Nigeria
Ajayi Abraham, Smith Stella Ifeanyi, Fowora Muinah, Bode-Sojobi Ibidunni Oreoluwa, Kalpy Julien Coulibaly, Adeleye Isaac Adeyemi
Pathogens and Global Health.2019; 113(6): 282. CrossRef -
Assembly mechanism of the α-pore–forming toxin cytolysin A from
Escherichia coli
Daniel Roderer, Rudi Glockshuber
Philosophical Transactions of the Royal Society B: Biological Sciences.2017; 372(1726): 20160211. CrossRef - Soluble Oligomers of the Pore-forming Toxin Cytolysin A from Escherichia coli Are Off-pathway Products of Pore Assembly
Daniel Roderer, Stephan Benke, Benjamin Schuler, Rudi Glockshuber
Journal of Biological Chemistry.2016; 291(11): 5652. CrossRef - The assembly dynamics of the cytolytic pore toxin ClyA
Stephan Benke, Daniel Roderer, Bengt Wunderlich, Daniel Nettels, Rudi Glockshuber, Benjamin Schuler
Nature Communications.2015;[Epub] CrossRef - Eha, a transcriptional regulator of hemolytic activity ofEdwardsiella tarda
Daqing Gao, Jing Cheng, Enjin Zheng, Yuhong Li, Zeye Shao, Zeyan Xu, Chengping Lu
FEMS Microbiology Letters.2014; 353(2): 132. CrossRef - Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains
Constance Oben Ayuk Enow, Jan Oscarsson, Nikola Zlatkov, Marie Westermark, Marylise Duperthuy, Sun Nyunt Wai, Bernt Eric Uhlin
BMC Microbiology.2014;[Epub] CrossRef
- Immunological Responses Induced by asd and wzy/asd Mutant Strains of Salmonella enterica serovar Typhimurium in BALB/c Mice
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Hong Hua Piao , Vo Thi Minh Tam , Hee Sam Na , Hyun Ju Kim , Phil Youl Ryu , Soo Young Kim , Joon Haeng Rhee , Hyon E. Choy , Suhng Wook Kim , Yeongjin Hong
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J. Microbiol. 2010;48(4):486-495. Published online August 20, 2010
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DOI: https://doi.org/10.1007/s12275-010-0023-z
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Attenuated bacteria have long been developed as vaccine candidates but can have some disadvantages, such as the potential for damage to immune organs due to insufficient clearance. To minimize these disadvantages, we generated Salmonella enterica serovar Typhimurium mutants SHJ2104 (asd::cm) and HTSaYA (wzy::km, asd::cm). The wzy gene codes for the O-antigen polymerase, which is involved in lipopolysaccharide (LPS) biosynthesis, and asd codes for aspartate ß- semialdehyde dehydrogenase, which participates in cell wall formation. The strains synthesized LPS with a short-chain length, and showed lower cytotoxicity and reduced intracellular proliferation in animal cells compared to wild-type bacteria. After oral infection, the mutants were cleared in immune tissues, including the Peyer’s patch, mesenteric lymph node, and spleen, within 5 days. The LD50 of the mutants in Balb/c mice was estimated to be 106 higher than wild-type bacteria when administered either via an oral or i.p. route, indicating that the two strains are highly attenuated. To compare the immune response to and protective effects of the mutants against wild-type bacterial infection, we inoculated the mutants into mice via an oral (1×1010 CFU) or i.p. (1×107 CFU) route once or twice at a two week interval. All immune responses, such as serum IgG and secretory IgA levels, cytokine production, and delayed hypersensitivity, were highly induced by two rounds of immunization. HTSaYA and SHJ2104 induced similar immune responses, and mice immunized with HTSaYA or SHJ2104 via an i.p. route were protected against wild-type Salmonella infection even at 100-fold of the LD50 (5×106 CFU). Taken together, these data indicate that HTSaYA and SHJ2104 could be developed as live attenuated Salmonella vaccine candidates.
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Citations
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Abubakar Siddique, Zining Wang, Haiyang Zhou, Linlin Huang, Chenghao Jia, Baikui Wang, Abdelaziz Ed-Dra, Lin Teng, Yan Li, Min Yue
Vaccines.2024; 12(9): 1067. CrossRef - Gut Microbiota Analysis and In Silico Biomarker Detection of Children with Autism Spectrum Disorder across Cohorts
Wenjuan Wang, Pengcheng Fu
Microorganisms.2023; 11(2): 291. CrossRef - Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae
Giarlã Cunha da Silva, Ciro César Rossi, Jéssica Nogueira Rosa, Newton Moreno Sanches, Daniela Lopes Cardoso, Yanwen Li, Adam A. Witney, Kate A. Gould, Patrícia Pereira Fontes, Anastasia J. Callaghan, Janine Thérèse Bossé, Paul Richard Langford, Denise Ma
Frontiers in Microbiology.2022;[Epub] CrossRef - VaximmutorDB: A Web-Based Vaccine Immune Factor Database and Its Application for Understanding Vaccine-Induced Immune Mechanisms
Kimberly Berke, Peter Sun, Edison Ong, Nasim Sanati, Anthony Huffman, Timothy Brunson, Fred Loney, Joseph Ostrow, Rebecca Racz, Bin Zhao, Zuoshuang Xiang, Anna Maria Masci, Jie Zheng, Guanming Wu, Yongqun He
Frontiers in Immunology.2021;[Epub] CrossRef - Efficacy of orally administered ginseng stem and leaf in chickens
Soyeon Park, Kwang-Yeal Lee, Youngjae Cho, Bokyoung Park, Kiju Kim, Na-Rae Lee, Dong-Gun Kim, Young-Hee Kim, Tae-Wook Hahn
Korean Journal of Veterinary Research.2015; 55(1): 1. CrossRef - Live attenuated vaccines for invasive Salmonella infections
Sharon M. Tennant, Myron M. Levine
Vaccine.2015; 33: C36. CrossRef - Attenuating gene expression (AGE) for vaccine development
David W Pascual, Zhiyong Suo, Ling Cao, Recep Avci, Xinghong Yang
Virulence.2013; 4(5): 384. CrossRef - Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection
Kiju Kim, Dooree Kim, Jisun Sun, Soyeon Park, Youngjae Cho, Hyun-Jeong Ko, Hong-Gu Joo, Tae-Wook Hahn
Korean Journal of Veterinary Research.2013; 53(2): 95. CrossRef - STM2209-STM2208 (opvAB): A Phase Variation Locus of Salmonella enterica Involved in Control of O-Antigen Chain Length
Ignacio Cota, Anne Béatrice Blanc-Potard, Josep Casadesús, Dipshikha Chakravortty
PLoS ONE.2012; 7(5): e36863. CrossRef - Sublingual vaccination with sonicated Salmonella proteins and mucosal adjuvant induces mucosal and systemic immunity and protects mice from lethal enteritis
CHING-FENG HUANG, TZEE-CHUNG WU, CHIA-CHAO WU, CHIN-CHENG LEE, WEN-TSUNG LO, KWEI-SHUAI HWANG, MU-LING HSU, HO-JEN PENG
APMIS.2011; 119(7): 468. CrossRef
- Expression of c-Myc Is Related to Host Cell Death Following Salmonella typhimurium Infection in Macrophage
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Jihyoun Seong , Hong Hua Piao , Phil Yeoul Ryu , Youn Uck Kim , Hyon E Choy , Yeongjin Hong
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J. Microbiol. 2009;47(2):214-219. Published online May 2, 2009
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DOI: https://doi.org/10.1007/s12275-008-0308-7
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It has been known that ornithine decarboxylase (ODC) induced by the binding of c-Myc to odc gene is closely linked to cell death. Here, we investigated the relationship between their expressions and cell death in macrophage cells following treatment with Salmonella typhimurium or lipopolysaccharide (LPS). ODC expression was increased by bacteria or LPS and repressed by inhibitors against mitogen-activated protein kinases (MAPKs) in Toll-like receptor 4 (TLR4) signaling pathway. In contrast, c-Myc protein level was increased after treatment with bacteria, but not by treatment with LPS or heat-killed bacteria although both bacteria and LPS increased the levels of c-myc mRNA to a similar extent. c-Myc protein level is dependent upon bacterial invasion because treatment with cytochalasin D (CCD), inhibitors of endocytosis, decreased c-Myc protein level. The cell death induced by bacteria was significantly decreased after treatment of CCD or c-Myc inhibitor, indicating that cell death by S. typhimurium infection is related to c-Myc, but not ODC. Consistent with this conclusion, treatment with bacteria mutated to host invasion did not increase c-Myc protein level and cell death rate. Taken together, it is suggested that induction of c-Myc by live bacterial infection is directly related to host cell death.
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Citations
Citations to this article as recorded by - G-quadruplex-targeting indoloquinoxaline derivative modulates host immunity against Leishmania donovani
Joyshree Karmakar, Sarmistha Pal, Maximilian Johannes Braun, Sk Jubayar Ahashan, Harald Schwalbe, Jyotirmayee Dash
NAR Molecular Medicine.2025;[Epub] CrossRef - Enhanced bacterial cancer therapy delivering therapeutic RNA interference of c-Myc
Jason S. Williams, Adam T. Higgins, Katie J. Stott, Carly Thomas, Lydia Farrell, Cleo S. Bonnet, Severina Peneva, Anna V. Derrick, Trevor Hay, Tianqi Wang, Claire Morgan, Sarah Dwyer, Joshua D’Ambrogio, Catherine Hogan, Matthew J. Smalley, Lee Parry, Paul
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Kaiyi Zhang, Mathias Hornef, Marcus Fulde
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Howard C. H. Yim, James C. B. Li, John C. H. Pong, Allan S. Y. Lau
Proceedings of the National Academy of Sciences.2011; 108(43): 17749. CrossRef
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