- Phylogenetic study of trichaptum inferred from nuclear ribosomal DNA sequences
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Ko, Kwon Soo , Hong, Soon Gyu , Jung, Hack Sung
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J. Microbiol. 1997;35(2):79-86.
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Abstract
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For the phylogenetic study of the genus Trichaptum, nuclear ribosomal DNA sequences from eight strains of four Trichaptium species were examined. Phylogenetic trees were constructed using molecular data on 18 rDNA and 5.8S rDNA and thei ITSs. Parsimony analyses of the Trichaptum species showed that T. biforme and T. laricinum made a monophyletic group respectively, suggesting that each species is phylogenetically independent. However, T. abietum represented a polyphyletic group and T. fusco-violaceum formed a polytomous group, suggesting that these species could be in the process of evolutionary differentiation. Examination of base substitutions of the 18S rRNA gene reveals that the C-T transition is most predominant and that there is a stronger transition bias between closely related organisms rather than between distantly related ones.
- Restriction and Transcription Maps of Mitochondrial DNA of Trimorphomyces papilionaceus
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Jeoung, Won Jin , Hong, Soon Gyu , Kang, Young Won , Jung, Hack Sung
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J. Microbiol. 1995;33(2):149-153.
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Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.
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