- Functional characterization of HigBA toxin-antitoxin system in an Arctic bacterium, Bosea sp. PAMC 26642
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Eunsil Choi , Ahhyun Huh , Changmin Oh , Jeong-Il Oh , Ho Young Kang , Jihwan Hwang
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J. Microbiol. 2022;60(2):192-206. Published online February 1, 2022
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DOI: https://doi.org/10.1007/s12275-022-1619-9
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 Abstract
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Toxin-antitoxin (TA) systems are growth-controlling genetic
elements consisting of an intracellular toxin protein and its
cognate antitoxin. TA systems have been spread among microbial
genomes through horizontal gene transfer and are
now prevalent in most bacterial and archaeal genomes. Under
normal growth conditions, antitoxins tightly counteract the
activity of the toxins. Upon stresses, antitoxins are inactivated,
releasing activated toxins, which induce growth arrest or cell
death. In this study, among nine functional TA modules in
Bosea sp. PAMC 26642 living in Arctic lichen, we investigated
the functionality of BoHigBA2. BohigBA2 is located close to
a genomic island and adjacent to flagellar gene clusters. The
expression of BohigB2 induced the inhibition of E. coli growth
at 37°C, which was more manifest at 18°C, and this growth
defect was reversed when BohigA2 was co-expressed, suggesting
that this BoHigBA2 module might be an active TA
module in Bosea sp. PAMC 26642. Live/dead staining and
viable count analyses revealed that the BoHigB2 toxin had
a bactericidal effect, causing cell death. Furthermore, we demonstrated
that BoHigB2 possessed mRNA-specific ribonuclease
activity on various mRNAs and cleaved only mRNAs
being translated, which might impede overall translation and
consequently lead to cell death. Our study provides the insight
to understand the cold adaptation of Bosea sp. PAMC 26642
living in the Arctic.
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Citations
Citations to this article as recorded by  - Evaluating the Contribution of the Predicted Toxin–Antitoxin System HigBA to Persistence, Biofilm Formation, and Virulence in Burkholderia pseudomallei
Itziar Chapartegui-González, Nittaya Khakhum, Jacob L. Stockton, Alfredo G. Torres, Igor E. Brodsky
Infection and Immunity.2022;[Epub] CrossRef - Chronicle of Research into Lichen-Associated Bacteria
Zichen He, Takeshi Naganuma
Microorganisms.2022; 10(11): 2111. CrossRef - Degradation of amoxicillin by newly isolated Bosea sp. Ads-6
Lei Yan, Ning Yan, Xi-Yan Gao, Ying Liu, Zhi-Pei Liu
Science of The Total Environment.2022; 828: 154411. CrossRef
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