- The Role of a Dark Septate Endophytic Fungus, Veronaeopsis simplex Y34, in Fusarium Disease Suppression in Chinese Cabbage
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Rida O. Khastini , Hiroyuki Ohta , Kazuhiko Narisawa
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J. Microbiol. 2012;50(4):618-624. Published online August 25, 2012
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DOI: https://doi.org/10.1007/s12275-012-2105-6
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The soil-inhabiting fungal pathogen Fusarium oxysporum has been an increasing threat to Chinese cabbage (Brassica campestris L.). A dark septate endophytic fungus, Veronaeopsis simplex Y34, isolated from Yaku Island, Japan, was evaluated in vitro for the ability to suppress Fusarium disease. Seedlings grown in the presence of the endophyte showed a 71% reduction in Fusarium wilt disease and still had good growth. The disease control was achieved through a synergetic effect involving a mechanical resistance created by a dense network of V. simplex Y34 hyphae, which colonized the host root, and siderophore production acting indirectly to induce a resistance mechanism in the plant. Changes in the relative abundance of the fungal communities in the soil as determined by fluorescently labelled T-RFs (terminal restriction fragments), appeared 3 weeks after application of the fungus. Results showed the dominance of V. simplex Y34, which became established in the rhizosphere and out-competed F. oxysporum.
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- Sphingopyxis granuli sp. nov., a [beta]-Glucosidase-Producing Bacterium in the Family Sphingomonadaceae in [alpha]-4 Subclass of the Proteobacteria
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Myung Kyum Kim , Wan-Taek Im , Hiroyuki Ohta , Myungjin Lee , Sung-Taik Lee
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J. Microbiol. 2005;43(2):152-157.
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DOI: https://doi.org/2169 [pii]
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Abstract
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Strain Kw07^T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07^T belongs to the [alpha]-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033^T (97.8%). Chemotaxonomic data revealed that strain Kw07^T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C_18:1 w7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07^T represents a distinct species. Based on these data, Kw07^T (= KCTC 12209^T = NBRC 100800^T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.
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