- The putative sensor histidine kinase VadJ coordinates development and sterigmatocystin production in Aspergillus nidulans
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Yanxia Zhao , Mi-Kyung Lee , Jieyin Lim , Heungyun Moon , Hee-Soo Park , Weifa Zheng , Jae-Hyuk Yu
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J. Microbiol. 2021;59(8):746-752. Published online July 5, 2021
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DOI: https://doi.org/10.1007/s12275-021-1055-2
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The VosA-VelB heterocomplex governs expression of several
genes associated with fungal development and secondary
metabolism. In this study, we have investigated the functions
of one of the VosA-VelB-activated developmental genes vadJ
in development and production of the mycotoxin sterigmatocystin
in the model fungus Aspergillus nidulans. The vadJ
gene is predicted to encode a 957-amino acid length protein
containing a highly conserved sensor histidine kinase domain.
The deletion of vosA or velB resulted in decreased mRNA
levels of vadJ throughout the life cycle, suggesting that VosA
and VelB are necessary for proper expression of vadJ. Nullifying
vadJ led to highly restricted colony growth, lowered formation
of asexual spores, and about two-fold reduction in
conidial viability. Conversely, the deletion of vadJ resulted in
elevated production of sexual fruiting bodies and sterigmatocystin.
These suggest that VadJ is necessary for proper coordination
of asexual and sexual development, and sterigmatocystin
production. In accordance with this idea, the deletion
of vadJ led to elevated mRNA levels of the two key sexual
developmental activators esdC and nsdD. In summary, the
putative sensor histidine kinase VadJ represses sexual development
and sterigmatocystin production, but activates
asexual development in A. nidulans.
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Citations
Citations to this article as recorded by  - Velvet regulators as master integrators of development, metabolism, and pathogenicity in filamentous fungi
Wanping Chen, Ye-Eun Son, He-Jin Cho, Yonghua Wang, Hee-Soo Park, Jae-Hyuk Yu, Joseph Heitman
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- Characterization of the velvet regulators in Aspergillus flavus
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Tae-Jin Eom , Heungyun Moon , Jae-Hyuk Yu , Hee-Soo Park
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J. Microbiol. 2018;56(12):893-901. Published online October 25, 2018
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DOI: https://doi.org/10.1007/s12275-018-8417-4
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964
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Abstract
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Fungal development and secondary metabolism are closely
associated via the activities of the fungal NK-kB-type velvet
regulators that are highly conserved in filamentous fungi.
Here, we investigated the roles of the velvet genes in the aflatoxigenic
fungus Aspergillus flavus. Distinct from other Aspergillus
species, the A. flavus genome contains five velvet genes,
veA, velB, velC, velD, and vosA. The deletion of velD blocks
the production of aflatoxin B1, but does not affect the formation
of sclerotia. Expression analyses revealed that vosA and
velB mRNAs accumulated at high levels during the late phase
of asexual development and in conidia. The absence of vosA
or velB decreased the content of conidial trehalose and the
tolerance of conidia to the thermal and UV stresses. In addition,
double mutant analyses demonstrated that VosA and
VelB play an inter-dependent role in trehalose biosynthesis
and conidial stress tolerance. Together with the findings of
previous studies, the results of the present study suggest that
the velvet regulators play the conserved and vital role in sporogenesis,
conidial trehalose biogenesis, stress tolerance, and
aflatoxin biosynthesis in A. flavus.
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