- Purification and Characterization of the α-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756
-
Ana Flávia Azevedo Carvalho , Maurício Boscolo , Roberto da Silva , Henrique Ferreira , Eleni Gomes
-
J. Microbiol. 2010;48(4):452-459. Published online August 20, 2010
-
DOI: https://doi.org/10.1007/s12275-010-9319-2
-
-
706
View
-
0
Download
-
9
Crossref
-
 Abstract
 PDF
-
Αn α-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70°C. Enzyme showed stability stable in the pH range of 3.0-9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60°C. In the presence of ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ this enzyme maintained 90-105% of its maximum activity and was inhibited by Cr3+, Ag+, and Hg2+. The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and α-PNPG. The Km measured for the α-glucosidase was 0.07 μM, with a Vmax of 318.0 μmol/min/mg.
-
Citations
Citations to this article as recorded by  - Biotechnological Potential of a Glucose-Tolerant β-Glucosidase from Rasamsonia composticola (β-GluRc) in Sugarcane Bagasse Saccharification
Isabela Pavão Vargas, Rodrigo Mattos Silva Galeano, Aline Pereira de Almeida, Ana Lorena de Oliveira Simas, João Victor Souza Ribeiro, Maria de Lourdes Teixeira de Moraes Polizeli, Douglas Chodi Masui, Giovana Cristina Giannesi, Roberto Ruller, Fabiana Fo
Waste and Biomass Valorization.2026; 17(8): 4723. CrossRef - Enzymatic characterization of the lignocellulolytic secretome of Penicillium Variabile wild strain from post-milling sugarcane bagasse in submerged fermentation
Janete Maria da Silva Alves, Renata Cândida Pinto Guerra, Maria Lúcia Gomes e Souza, Junio Cota Silva, Márcio Antônio Silva Pimenta, Maria Carolina Soares Lopes, Henrique Maia Valerio
Evidência.2026; 26: e36808. CrossRef - Enhanced Production, Purification, and Characterization of α-Glucosidase from NTG-Mutagenized Aspergillus niger for Industrial Applications
Bowei Yao, Qian Liu, Junjie Xiong, Guangming Feng, Zhongyi Chang, Hongliang Gao
Catalysts.2025; 15(5): 450. CrossRef - Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
Process Biochemistry.2024; 136: 109. CrossRef - Chemical Constituents, Antioxidant, and α-Glucosidase Inhibitory Activities of Different Fermented Gynostemma Pentaphyllum Leaves and Untargeted Metabolomic Measurement of the Metabolite Variation
Xuechun Zhang, Shi Li, Zhibin Zhang, Kin Weng Kong, Zhenxing Wang, Xiahong He
Antioxidants.2023; 12(8): 1505. CrossRef - The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus
Shenglin Hu, Zhefan Wang, Dongmei Wang, Jichao Wang, Jiong Hong
3 Biotech.2021;[Epub] CrossRef - Production and Catalytic Properties of Amylases fromLichtheimia ramosaandThermoascus aurantiacusby Solid-State Fermentation
Ana Paula Aguero de Oliveira, Maria Alice Silvestre, Nayara Fernanda Lisboa Garcia, Heloíza Ferreira Alves-Prado, André Rodrigues, Marcelo Fossa da Paz, Gustavo Graciano Fonseca, Rodrigo Simões Ribeiro Leite
The Scientific World Journal.2016; 2016: 1. CrossRef - Purification and characterization of a novel α-glucosidase from Malbranchea cinnamomea
Q. J. Yan, P. Han, S. Q. Yang, Z. Q. Jiang
Biotechnology Letters.2015; 37(6): 1279. CrossRef - Biochemical properties of an extracellular trehalase from Malbranchea pulchella var. Sulfurea
Marita Gimenez Pereira, Luis Henrique Souza Guimarães, Rosa Prazeres Melo Furriel, Maria de Lourdes Teixeira de Moraes Polizeli, Hector Francisco Terenzi, João Atílio Jorge
The Journal of Microbiology.2011; 49(5): 809. CrossRef
|
MSK
