- Plasminogen Activator Inhibitor Type 1 Expression Induced by Lipopolysaccharide of Porphyromonas gingivalis in Human Gingival Fibroblast
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Hee Sam Na , Eun J. Lim , So Y. Jeong , Mi H. Ryu , Mi Hee Park , Jin Chung
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J. Microbiol. 2014;52(2):154-160. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3022-7
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In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved in PAI-1 expression induced by Porphyromonas gingivalis LPS (Pg LPS) in human gingival fibroblasts (HGF). When HGFs were treated with Pg LPS, both PAI-1 mRNA expression and PAI-1 protein were induced in a dose-dependent manner. Pg LPS induced NF-κB activation and the expressions of PAI-1 mRNA and protein were suppressed by pretreating with a NF-κB inhibitor. Pg LPS also induced ERK, p38, and JNK activation, and Pg LPS-induced PAI-1 expression was inhibited by ERK/p38/JNK inhibitor pretreatment. In conclusion, Pg LPS induced PAI-1 expression through NF-κB and MAP kinases activation in HGF.
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Citations
Citations to this article as recorded by  - Regulatory roles of gingival fibroblasts in periodontal homeostasis and inflammatory progression
Sijia Liu, Zixuan Zhao, Na Liu, Qing Liu
Archives of Oral Biology.2026; 189: 106669. CrossRef - Impaired fibrinolysis in patients with atrial fibrillation and elevated circulating lipopolysaccharide
Marcin Sadowski, Michał Ząbczyk, Anetta Undas
Journal of Thrombosis and Thrombolysis.2024; 57(5): 842. CrossRef - Impact of scaling and root planing on salivary and serum plasminogen activator inhibitor‐1 expression in patients with periodontitis with and without type 2 diabetes mellitus
Sanjeela R. Guru, Suchetha Aghanashini
Journal of Periodontology.2023; 94(1): 20. CrossRef - Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update
Aditi Chopra, Subraya G. Bhat, Karthik Sivaraman
Journal of Oral Microbiology.2020; 12(1): 1801090. CrossRef - Periodontal Pathogens and Neuropsychiatric Health
Abhishek Wadhawan, Mark A. Reynolds, Hina Makkar, Alison J. Scott, Eileen Potocki, Andrew J. Hoisington, Lisa A. Brenner, Aline Dagdag, Christopher A. Lowry, Yogesh Dwivedi, Teodor T. Postolache
Current Topics in Medicinal Chemistry.2020; 20(15): 1353. CrossRef - MicroRNA-212-5p regulates the inflammatory response of periodontal ligament cells by targeting myeloid differentiation factor 88
Bo Hua, Junbo Xiang, Lin Guo, Dongling Lu
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Aditi Chopra, Karthik Sivaraman
Critical Reviews in Microbiology.2019; 45(5-6): 514. CrossRef - Periodontitis induced by Porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response
Vincent Blasco-Baque, Lucile Garidou, Céline Pomié, Quentin Escoula, Pascale Loubieres, Sandrine Le Gall-David, Mathieu Lemaitre, Simon Nicolas, Pascale Klopp, Aurélie Waget, Vincent Azalbert, André Colom, Martine Bonnaure-Mallet, Philippe Kemoun, Matteo
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Kah Yan How, Keang Peng Song, Kok Gan Chan
Frontiers in Microbiology.2016;[Epub] CrossRef - Porphyromonas gingivalis: Major Periodontopathic Pathogen Overview
Jaroslav Mysak, Stepan Podzimek, Pavla Sommerova, Yelena Lyuya-Mi, Jirina Bartova, Tatjana Janatova, Jarmila Prochazkova, Jana Duskova
Journal of Immunology Research.2014; 2014: 1. CrossRef
- Immunological Responses Induced by asd and wzy/asd Mutant Strains of Salmonella enterica serovar Typhimurium in BALB/c Mice
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Hong Hua Piao , Vo Thi Minh Tam , Hee Sam Na , Hyun Ju Kim , Phil Youl Ryu , Soo Young Kim , Joon Haeng Rhee , Hyon E. Choy , Suhng Wook Kim , Yeongjin Hong
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J. Microbiol. 2010;48(4):486-495. Published online August 20, 2010
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DOI: https://doi.org/10.1007/s12275-010-0023-z
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819
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Attenuated bacteria have long been developed as vaccine candidates but can have some disadvantages, such as the potential for damage to immune organs due to insufficient clearance. To minimize these disadvantages, we generated Salmonella enterica serovar Typhimurium mutants SHJ2104 (asd::cm) and HTSaYA (wzy::km, asd::cm). The wzy gene codes for the O-antigen polymerase, which is involved in lipopolysaccharide (LPS) biosynthesis, and asd codes for aspartate ß- semialdehyde dehydrogenase, which participates in cell wall formation. The strains synthesized LPS with a short-chain length, and showed lower cytotoxicity and reduced intracellular proliferation in animal cells compared to wild-type bacteria. After oral infection, the mutants were cleared in immune tissues, including the Peyer’s patch, mesenteric lymph node, and spleen, within 5 days. The LD50 of the mutants in Balb/c mice was estimated to be 106 higher than wild-type bacteria when administered either via an oral or i.p. route, indicating that the two strains are highly attenuated. To compare the immune response to and protective effects of the mutants against wild-type bacterial infection, we inoculated the mutants into mice via an oral (1×1010 CFU) or i.p. (1×107 CFU) route once or twice at a two week interval. All immune responses, such as serum IgG and secretory IgA levels, cytokine production, and delayed hypersensitivity, were highly induced by two rounds of immunization. HTSaYA and SHJ2104 induced similar immune responses, and mice immunized with HTSaYA or SHJ2104 via an i.p. route were protected against wild-type Salmonella infection even at 100-fold of the LD50 (5×106 CFU). Taken together, these data indicate that HTSaYA and SHJ2104 could be developed as live attenuated Salmonella vaccine candidates.
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Citations
Citations to this article as recorded by - The Evolution of Vaccines Development across Salmonella Serovars among Animal Hosts: A Systematic Review
Abubakar Siddique, Zining Wang, Haiyang Zhou, Linlin Huang, Chenghao Jia, Baikui Wang, Abdelaziz Ed-Dra, Lin Teng, Yan Li, Min Yue
Vaccines.2024; 12(9): 1067. CrossRef - Gut Microbiota Analysis and In Silico Biomarker Detection of Children with Autism Spectrum Disorder across Cohorts
Wenjuan Wang, Pengcheng Fu
Microorganisms.2023; 11(2): 291. CrossRef - Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae
Giarlã Cunha da Silva, Ciro César Rossi, Jéssica Nogueira Rosa, Newton Moreno Sanches, Daniela Lopes Cardoso, Yanwen Li, Adam A. Witney, Kate A. Gould, Patrícia Pereira Fontes, Anastasia J. Callaghan, Janine Thérèse Bossé, Paul Richard Langford, Denise Ma
Frontiers in Microbiology.2022;[Epub] CrossRef - VaximmutorDB: A Web-Based Vaccine Immune Factor Database and Its Application for Understanding Vaccine-Induced Immune Mechanisms
Kimberly Berke, Peter Sun, Edison Ong, Nasim Sanati, Anthony Huffman, Timothy Brunson, Fred Loney, Joseph Ostrow, Rebecca Racz, Bin Zhao, Zuoshuang Xiang, Anna Maria Masci, Jie Zheng, Guanming Wu, Yongqun He
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Soyeon Park, Kwang-Yeal Lee, Youngjae Cho, Bokyoung Park, Kiju Kim, Na-Rae Lee, Dong-Gun Kim, Young-Hee Kim, Tae-Wook Hahn
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Sharon M. Tennant, Myron M. Levine
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David W Pascual, Zhiyong Suo, Ling Cao, Recep Avci, Xinghong Yang
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Ignacio Cota, Anne Béatrice Blanc-Potard, Josep Casadesús, Dipshikha Chakravortty
PLoS ONE.2012; 7(5): e36863. CrossRef - Sublingual vaccination with sonicated Salmonella proteins and mucosal adjuvant induces mucosal and systemic immunity and protects mice from lethal enteritis
CHING-FENG HUANG, TZEE-CHUNG WU, CHIA-CHAO WU, CHIN-CHENG LEE, WEN-TSUNG LO, KWEI-SHUAI HWANG, MU-LING HSU, HO-JEN PENG
APMIS.2011; 119(7): 468. CrossRef
- Effect of Iron on Cytolysin A Expression in Salmonella enterica serovar Typhi
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Jinghua Cui , Honghua Piao , Shen Jin , Hee Sam Na , Yeongjin Hong , Hyon E Choy , Phil Youl Ryu
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J. Microbiol. 2009;47(4):479-485. Published online September 9, 2009
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DOI: https://doi.org/10.1007/s12275-009-0039-4
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698
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Previously, a novel protein ClyA (Cytolysin A) has been identified in Escherichia coli K-12, Salmonella enterica serovars Typhi and Paratyphi A and Shigella. Salmonella spp. synthesize substantial amounts of ClyA upon infection of the human host, although the mechanism by which ClyA is induced in vivo is unclear. Since environmental signals could control the expression of virulence determinants, ClyA expression in S. Typhi Ty2 was tested by Western blotting in the presence of normal pooled human serum (NPS). The level of ClyA expression increased in the presence of NPS in a concentration-dependent manner. RPMI 1640 medium similarly induced ClyA expression. ClyA expression was inversely proportional to the concentration of iron in RPMI medium. Therefore, we speculated that iron inhibited the expression of ClyA in S. Typhi Ty2, and free iron depletion may be one of the causes of S. Typhi-mediated induction of ClyA in vivo. Transcription from a clyA-lacZ fusion gene decreased as iron concentration increased, but not as significantly as the ClyA protein expression. It is concluded that the regulatory effect of iron on clyA expression is mainly at translational level.
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Citations
Citations to this article as recorded by - Expression of Bacteroides fragilis hemolysins in vivo and role of HlyBA in an intra‐abdominal infection model
Leandro A. Lobo, Audrey L. Jenkins, C. Jeffrey Smith, Edson R. Rocha
MicrobiologyOpen.2013; 2(2): 326. CrossRef - Molecular cloning and characterization of clyA genes in various serotypes of Salmonella enterica
Lan Ji Huang, Jinghua Cui, Hong Hua Piao, Yeongjin Hong, Hyon E. Choy, Phil Youl Ryu
The Journal of Microbiology.2010; 48(5): 663. CrossRef
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