Journal of Microbiology

Hao Zhang

5 Articles

Development of a strategy for the screening of α-glucosidase-producing microorganisms: Bo Zhou+ , Nan Huang+ , Wei Zeng+ , Hao Zhang , Guiguang Chen , Zhiqun Liang; J. Microbiol. 2020;58(2):163-172. Published online January 29, 2020; DOI: https://doi.org/10.1007/s12275-020-9267-4

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α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-D-methylglucoside (AMG) in glass plates was tested for the primary screening of α-glucosidaseproducing strains. First, α-glucosidase-producing Aspergillus niger strains were selected on plates containing EY and AMG based on transparent zone formation resulting from the solubilization of EY by the hydrolyzed product. Conventional
methods
that use trypan blue (TB) and p-nitrophenyl-α-Dglucopyranoside (pPNP) as indicators were then compared with the new strategy. The results showed that EY-containing plates provide the advantages of low price and higher specificity for the screening of α-glucosidase-producing strains. We then evaluated the correlation between the hydrolytic activity of α-glucosidase and diffusion distance, and found that good linearity could be established within a 6–75 U/ml enzyme concentration range. Finally, the hydrolytic and transglycosylation activities of α-glucosidase obtained from the target isolates were determined by EY plate assay and 3,5- dinitrosalicylic acid-Saccharomyces cerevisiae assay, respectively. The results showed that the diameter of the transparent zone varied among isolates was positively correlated with α-glucosidase hydrolytic activity, while good linearity could also be established between α-glucosidase transglycosylation activity and non-fermentable reducing sugars content. With this strategy, 7 Aspergillus niger mutants with high yield of α-glucosidase from 200 obvious single colonies on the primary screen plate were obtained.

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Enhanced Production, Purification, and Characterization of α-Glucosidase from NTG-Mutagenized Aspergillus niger for Industrial Applications
Bowei Yao, Qian Liu, Junjie Xiong, Guangming Feng, Zhongyi Chang, Hongliang Gao
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Molecular Docking and Dynamics Simulations Reveal the Antidiabetic Potential of a Novel Fucoxanthin Derivative from Chnoospora minima
Sachini Sigera, Kavindu D. Theekshana, Sathmi G. Dinanja, Pasindu Eranga, Nayanatharie Karunathilake, Shamali Abeywardhana, Laksiri Weerasinghe, Tharindu Senapathi, Dinithi C. Peiris
Marine Drugs.2025; 23(12): 471. CrossRef
Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
Process Biochemistry.2024; 136: 109. CrossRef
Development of a PMA‐LAMP visual detection assay for viable Cronobacter sakazakii
Qiming Chen, Yang Yu, Xiaodi Chen, Fangming Tu, Peng Wang, Junyi Huang, Zhanmin Liu
International Journal of Dairy Technology.2024; 77(2): 427. CrossRef
Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation
Chunxu Jiang, Han Wang, Menghan Liu, Li Wang, Ruwen Yang, Peng Wang, Zongmei Lu, Yong Zhou, Zhiming Zheng, Genhai Zhao
Applied Microbiology and Biotechnology.2022; 106(21): 6993. CrossRef
Cloning and characterization of a recombinant α-glucosidase from Ensifer adhaerens NBRC 100388 and evaluation of its glucosyl transfer activity
Tatsuya Suzuki, Miyu Fukaya, Kazuki Takahashi, Michiki Takeuchi, Ryotaro Hara, Jun Ogawa, Makoto Ueda
Biocatalysis and Agricultural Biotechnology.2020; 30: 101837. CrossRef

Gastrointestinal microbiota alteration induced by Mucor circinelloides in a murine model: Katherine D. Mueller , Hao Zhang , Christian R. Serrano , R. Blake Billmyre , Eun Young Huh , Philipp Wiemann , Nancy P. Keller , Yufeng Wang , Joseph Heitman , Soo Chan Lee; J. Microbiol. 2019;57(6):509-520. Published online May 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8682-x

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Abstract PDF

Mucor circinelloides is a pathogenic fungus and etiologic agent of mucormycosis. In 2013, cases of gastrointestinal illness after yogurt consumption were reported to the US FDA, and the producer found that its products were contaminated with Mucor. A previous study found that the Mucor strain isolated from an open contaminated yogurt exhibited virulence in a murine systemic infection model and showed that this strain is capable of surviving passage through the gastrointestinal tract of mice. In this study, we isolated another Mucor strain from an unopened yogurt that is closely related but distinct from the first Mucor strain and subsequently examined if Mucor alters the gut microbiota in a murine host model. DNA extracted from a ten-day course of stool samples was used to analyze the microbiota in the gastrointestinal tracts of mice exposed via ingestion of Mucor spores. The bacterial 16S rRNA gene and fungal ITS1 sequences obtained were used to identify taxa of each kingdom. Linear regressions revealed that there are changes in bacterial and fungal abundance in the gastrointestinal tracts of mice which ingested Mucor. Furthermore, we found an increased abundance of the bacterial genus Bacteroides and a decreased abundance of the bacteria Akkermansia muciniphila in the gastrointestinal tracts of exposed mice. Measurements of abundances show shifts in relative levels of multiple bacterial and fungal taxa between mouse groups. These findings suggest that exposure of the gastrointestinal tract to Mucor can alter the microbiota and, more importantly, illustrate an interaction between the intestinal mycobiota and bacteriota. In addition, Mucor was able to induce increased permeability in epithelial cell monolayers in vitro, which might be indicative of unstable intestinal barriers. Understanding how the gut microbiota is shaped is important to understand the basis of potential methods of treatment for gastrointestinal illness. How the gut microbiota changes in response to exposure, even by pathogens not considered to be causative agents of food-borne illness, may be important to how commercial food producers prevent and respond to contamination of products aimed at the public. This study provides evidence that the fungal microbiota, though understudied, may play an important role in diseases of the human gut.

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Ayşen İkkan, Osman Merdan, Seçil Ak-Aksoy, Zeinep Chavouz Ametoglou, İmran Sağlık, Hazel Öztürk-Belik, Büşra Çalışır, Özlem Saraydaroğlu, Samet Kızıl, Beyhan Bülbül, Esra Kazak, Beyza Ener
Frontiers in Cellular and Infection Microbiology.2026;[Epub] CrossRef
Microbe on the move: Akkermansia in infectious diseases and emerging roles in gynecological health
Stephanie M. Marroquin, Kelly S. Doran, Michael Y. Galperin
Journal of Bacteriology.2026;[Epub] CrossRef
Yeasts in the gastrointestinal tract
Katherine D Mueller, Soo Chan Lee, Jung Won Hee
FEMS Yeast Research.2026;[Epub] CrossRef
Cytokine Profile and Oxidative Patterns in Murine Models of Disseminated Infection by Mucorales Species
Hiram Villanueva-Lozano, Martín García-Juárez, Adrián G. Rosas-Taraco, Rogelio de J. Treviño-Rangel, Gloria M. González
Pathogens.2025; 14(10): 1036. CrossRef
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Mostafa.A. Sayed, S.M.A. El-Gamal, Alaa Mohsen, M. Ramadan, Mona M. Wetwet, Noha M. Deghiedy, Ahmed E. Swilem, M.M. Hazem
Construction and Building Materials.2024; 413: 134909. CrossRef
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Yejia Zhang, Hanchi Zhang, Tian Xu, Lingbing Zeng, Fadi Liu, Xiaotian Huang, Qiong Liu
The FEBS Journal.2024; 291(8): 1615. CrossRef
Different fungal signatures in ALD and MAFLD
Daya Zhang, Qi Wang, Da Li, Chen Chen, Yanting Lv, Shimei Huang, Fan Zeng, Xianfeng Huang, Fengjiao Mao, Feihu Bai
Frontiers in Microbiology.2024;[Epub] CrossRef
Interaction between intestinal mycobiota and microbiota shapes lung inflammation
Youxia Wang, Fang He, Bingnan Liu, Xiaoyan Wu, Ziyi Han, Xuefei Wang, Yuexia Liao, Jielin Duan, Wenkai Ren
iMeta.2024;[Epub] CrossRef
Characterisation of the surface growth of Mucor circinelloides in cheese agar media using predictive mathematical models
Martina Koňuchová, Agáta Boháčiková, Ľubomír Valík
Heliyon.2024; 10(9): e30812. CrossRef
Landscape of the gut mycobiome dynamics during pregnancy and its relationship with host metabolism and pregnancy health
Yuanqing Fu, Wanglong Gou, Ping Wu, Yuwei Lai, Xinxiu Liang, Ke Zhang, Menglei Shuai, Jun Tang, Zelei Miao, Jieteng Chen, Jiaying Yuan, Bin Zhao, Yunhaonan Yang, Xiaojuan Liu, Yayi Hu, An Pan, Xiong-Fei Pan, Ju-Sheng Zheng
Gut.2024; 73(8): 1302. CrossRef
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Chenguang Niu, Ye Tu, Qiaoqiao Jin, Zhanyi Chen, Keyong Yuan, Min Wang, Pengfei Zhang, Junyuan Luo, Hao Li, Yueyi Yang, Xiaoyu Liu, Mengying Mao, Ting Dong, Wenduo Tan, Xuchen Hu, Yihuai Pan, Lili Hou, Rui Ma, Zhengwei Huang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
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M. Ramadan, Mohamed Kohail, Aref A. Abadel, Yousef R. Alharbi, Ahmed M. Soliman, Alaa Mohsen
Journal of Materials Research and Technology.2023; 25: 3998. CrossRef
Evaluation of Mechanical Performance, Porous Structure and Self-Cleaning Behavior for Hydrothermally Cured Cementitious Composites Containing De-Aluminated Metakaolin Waste and Tio2 Nanoparticles
Alaa Mohsen, Mohamed Kohail, Aref abadel, Yousef Alharbi, Moncef L. Nehdi, Mohammed Ramadan
SSRN Electronic Journal .2022;[Epub] CrossRef
The role of gut mycobiome in health and diseases
Lin Zhang, Hui Zhan, Wenye Xu, Shuai Yan, Siew C. Ng
Therapeutic Advances in Gastroenterology.2021;[Epub] CrossRef
Mycobiome and Cancer: What Is the Evidence?
Natalia Vallianou, Dimitris Kounatidis, Gerasimos Socrates Christodoulatos, Fotis Panagopoulos, Irene Karampela, Maria Dalamaga
Cancers.2021; 13(13): 3149. CrossRef
Caenorhabditis elegans as an Infection Model for Pathogenic Mold and Dimorphic Fungi: Applications and Challenges
Chukwuemeka Samson Ahamefule, Blessing C. Ezeuduji, James C. Ogbonna, Anene N. Moneke, Anthony C. Ike, Cheng Jin, Bin Wang, Wenxia Fang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Crossing Kingdoms: How the Mycobiota and Fungal-Bacterial Interactions Impact Host Health and Disease
William Santus, Jason R. Devlin, Judith Behnsen, Karen M. Ottemann
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Sandeep Vellanki, R. Blake Billmyre, Alejandra Lorenzen, Micaela Campbell, Broderick Turner, Eun Young Huh, Joseph Heitman, Soo Chan Lee, Michael Lorenz
mBio.2020;[Epub] CrossRef
From Birth and Throughout Life: Fungal Microbiota in Nutrition and Metabolic Health
William D. Fiers, Irina Leonardi, Iliyan D. Iliev
Annual Review of Nutrition.2020; 40(1): 323. CrossRef
Understanding Mucormycoses in the Age of “omics”
Alexandra Y. Soare, Tonya N. Watkins, Vincent M. Bruno
Frontiers in Genetics.2020;[Epub] CrossRef
Eukaryotic and Prokaryotic Microbiota Interactions
Aly Kodio, Estelle Menu, Stéphane Ranque
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Drug-Resistant Epimutants Exhibit Organ-Specific Stability and Induction during Murine Infections Caused by the Human Fungal Pathogen Mucor circinelloides
Zanetta Chang, Joseph Heitman, Antonio Di Pietro
mBio.2019;[Epub] CrossRef

Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding: Fengwei Tian , Feifei Chi , Gang Wang , Xiaoming Liu , Qiuxiang Zhang , Yongquan Chen , Hao Zhang , Wei Chen; J. Microbiol. 2015;53(12):856-863. Published online December 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5239-5

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Abstract PDF

Lactobacillus rhamnosus CCFM1107 was screened for high antioxidative activity from 55 lactobacilli. The present study attempted to explore the protective properties of L. rhamnosus CCFM1107 in alcoholic liver injury. A mouse model was induced by orally feeding alcohol when simultaneously treated with L. rhamnosus CCFM1107, the drug Hu-Gan- Pian (HGP), L. rhamnosus GG (LGG), and L. plantarum CCFM1112 for 3 months. Biochemical analysis was performed for both serum and liver homogenate. Detailed intestinal flora and histological analyses were also carried out. Our results indicated that the administration of L. rhamnosus CCFM1107 significantly inhibited the increase in the levels of serum aminotransferase and endotoxin, as well as the levels of triglyceride (TG) and cholesterol (CHO) in the serum and in the liver. Glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were elevated while the levels of malondialdehyde (MDA) were decreased. The enteric dysbiosis caused by alcohol was restored by increasing the numbers of both lactobacilli and bifidobacteria and decreasing the numbers of both enterococci and enterobacter. Histological analysis confirmed the protective effect of L. rhamnosus CCFM1107. Compared with the other lactobacilli and to the drug Hu-Gan-Pian, there is a high chance that L. rhamnosus CCFM1107 provides protective effects on alcoholic liver injury by reducing oxidative stress and restoring the intestinal flora.

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Journal of Microbiology and Biotechnology.2024; 34(1): 149. CrossRef
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Phytomedicine.2024; 135: 156113. CrossRef
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Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
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Shokufeh Beglari, Sepideh Fereshteh, Mahnaz Milani, Niloofar Rezaie, Mahdi Rohani
Food Bioscience.2023; 56: 103202. CrossRef
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Frontiers in Bioscience-Landmark.2023;[Epub] CrossRef
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Wei Ye, Zengqiang Chen, Zhuoqi He, Haochen Gong, Jin Zhang, Jiaju Sun, Shanshan Yuan, Junjie Deng, Yanlong Liu, Aibing Zeng
Nutrients.2023; 15(4): 845. CrossRef
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Frontiers in Pharmacology.2023;[Epub] CrossRef
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Xi Shu, Jing Wang, Liang Zhao, Jian Wang, Pengjie Wang, Feng Zhang, Ran Wang
Heliyon.2023; 9(7): e17878. CrossRef
Recent Perspective of Lactobacillus in Reducing Oxidative Stress to Prevent Disease
Tingting Zhao, Haoran Wang, Zhenjiang Liu, Yang Liu, DeJi, Bin Li, Xiaodan Huang
Antioxidants.2023; 12(3): 769. CrossRef
Costunolide protects against alcohol‐induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro
Jingxin Mao, Honghong Zhan, Fancheng Meng, Guowei Wang, Dan Huang, Zhihua Liao, Min Chen
Phytotherapy Research.2022; 36(3): 1268. CrossRef
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Hong Zhao, Lingzhou Kong, Mengting Shao, Jiayue Liu, Changhai Sun, Changxu Li, Yanyan Wang, Xue Chai, Yuliang Wang, Yu Zhang, Xiaoliang Li
Journal of Ethnopharmacology.2022; 292: 115225. CrossRef
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Qinjian Wang, Jiangmin Shi, Min Zhao, Gaoyi Ruan, Zebin Dai, Yilang Xue, Dibang Shi, Changlong Xu, Ouyue Yu, Fangyan Wang, Zhanxiong Xue
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Nutrients.2021; 13(6): 1875. CrossRef
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Journal of Functional Foods.2020; 72: 104045. CrossRef
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Food & Function.2020; 11(7): 6115. CrossRef
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Dishen Chen, Thanh H. Le, Haleh Shahidipour, Scott A. Read, Golo Ahlenstiel
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Hye In Kim, Jeon-Kyung Kim, Jae-Young Kim, Se-Eun Jang, Myung Joo Han, Dong-Hyun Kim
Nutrition Research.2019; 67: 78. CrossRef
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Journal of Functional Foods.2019; 59: 234. CrossRef
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Lei Ding, Yihang Gong, Zhengfei Yang, Baojia Zou, Xialei Liu, Baimeng Zhang, Jian Li
Digestive Diseases and Sciences.2019; 64(10): 2867. CrossRef
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PLOS ONE.2019; 14(9): e0222339. CrossRef
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Eun Kyung Kwon, Geum-Dan Kang, Won-Kyeong Kim, Myung Joo Han, Dong-Hyun Kim
Journal of Functional Foods.2017; 38: 389. CrossRef
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Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults: Hao Zhang , Jing Sun , Xianting Liu , Chuan Hong , Yuanbo Zhu , Aiping Liu , Siqi Li , Huiyuan Guo , Fazheng Ren; J. Microbiol. 2013;51(6):777-782. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3279-2

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Abstract PDF

Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 1010 CFU of LC01 each day for the 4-week treatment period, and then consumed placebo in the next treatment period, separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichia coli and increase in Lactobacillus, Bifidobacterium, and Roseburia intestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.

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Minerva Gastroenterology.2026;[Epub] CrossRef
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Journal of Microbiology and Biotechnology.2020; 30(10): 1480. CrossRef
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European Journal of Nutrition.2020; 59(4): 1751. CrossRef
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Nutrients.2020; 12(6): 1776. CrossRef
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Probiotics and Antimicrobial Proteins.2020; 12(3): 952. CrossRef
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Nutrients.2019; 11(9): 2089. CrossRef
“Physicochemical, immunomodulatory and safety aspects of milks fermented with Lactobacillus paracasei isolated from kefir”
Ana A. Bengoa, Carolina Iraporda, Leonardo B. Acurcio, Sávio H. de Cicco Sandes, Karen Costa, Gabriele Moreira Guimarães, Rosa M. Esteves Arantes, Elisabeth Neumann, Álvaro Cantini Nunes, Jaques R. Nicoli, Graciela L. Garrote, Analía G. Abraham
Food Research International.2019; 123: 48. CrossRef
Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
Willian Ribeiro, Marco Vinolo, Leandro Calixto, Caroline Ferreira
BIO-PROTOCOL.2018;[Epub] CrossRef
RoseburiaSpp.: A Marker of Health?
Zohreh Tamanai-Shacoori, Imen Smida, Latifa Bousarghin, Olivier Loreal, Vincent Meuric, Shao Bing Fong, Martine Bonnaure-Mallet, Anne Jolivet-Gougeon
Future Microbiology.2017; 12(2): 157. CrossRef
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Ruby Yadav, Pratyoosh Shukla
Critical Reviews in Food Science and Nutrition.2017; 57(15): 3233. CrossRef
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Junpei Nakase, Yuuichi Ukawa, Syoji Takemoto, Takayoshi Kubo, Yuko M Sagesaka, Ayako Aoki-Yoshida, Mamoru Totsuka
Bioscience, Biotechnology, and Biochemistry.2017; 81(6): 1099. CrossRef
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Esther Nova, Fátima Pérez de Heredia, Sonia Gómez‐Martínez, Ascensión Marcos
Nutrition in Clinical Practice.2016; 31(3): 387. CrossRef
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Gregor Reid
International Dairy Journal.2015; 49: 16. CrossRef
Fate, activity, and impact of ingested bacteria within the human gut microbiota
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Trends in Microbiology.2015; 23(6): 354. CrossRef
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Safety Evaluation of Lactobacillus paracasei subsp. paracasei LC-01, Probiotic Bacterium: Hao Zhang , Yu Wang , Jing Sun , Zirui Guo , Huiyuan Guo , Fazheng Ren; J. Microbiol. 2013;51(5):633-638. Published online October 31, 2013; DOI: https://doi.org/10.1007/s12275-013-3336-x

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Abstract PDF

The safety of Lactobacillus paracasei subsp. paracasei LC-01 was evaluated for its use as a potential probiotic. In our in vitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. The results showed that the strain was sensitive to all tested antibiotics and did not produce biogenic amine except for tyramine. The oral toxicity of this strain was evaluated in Balb/C mice. One hundred mice were divided into 10 groups. Four groups were administered 0, 108, 109, or 1010 CFU/mouse per day dissolved in saline solution respectively, for 28 days. Three groups were injected intraperitoneally with 109 CFU/mouse dissolved in saline solution, and were killed 2, 5, and 10 days after injection. The last 3 groups were injected with the vehicle as controls respectively. The results showed that oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. Intraperitoneal administration caused a significant translocation to liver, spleen and kidney. However, this translocation did not cause illness or death throughout the experiment. The results suggest that L. paracasei subsp. paracasei LC-01 is likely to be safe for human consumption.

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