- Antiviral Activity Against SARS‑CoV‑2 Variants Using in Silico and in Vitro Approaches
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Hee-Jung Lee , Hanul Choi , Aleksandra Nowakowska , Lin-Woo Kang , Minjee Kim , Young Bong Kim
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J. Microbiol. 2023;61(7):703-711. Published online June 26, 2023
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DOI: https://doi.org/10.1007/s12275-023-00062-4
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emergence in 2019 led to global health crises and the persistent
risk of viral mutations. To combat SARS-CoV-2 variants, researchers have explored new approaches to identifying
potential targets for coronaviruses. This study aimed to identify SARS-CoV-2 inhibitors using drug repurposing. In silico
studies and network pharmacology were conducted to validate targets and coronavirus-associated diseases to select potential
candidates, and in vitro assays were performed to evaluate the antiviral effects of the candidate drugs to elucidate the
mechanisms of the viruses at the molecular level and determine the effective antiviral drugs for them. Plaque and cytopathic
effect reduction were evaluated, and real-time quantitative reverse transcription was used to evaluate the antiviral activity
of the candidate drugs against SARS-CoV-2 variants in vitro. Finally, a comparison was made between the molecular docking
binding affinities of fenofibrate and remdesivir (positive control) to conventional and identified targets validated from
protein–protein interaction (PPI). Seven candidate drugs were obtained based on the biological targets of the coronavirus,
and potential targets were identified by constructing complex disease targets and PPI networks. Among the candidates,
fenofibrate exhibited the strongest inhibition effect 1 h after Vero E6 cell infection with SARS-CoV-2 variants. This study
identified potential targets for coronavirus disease (COVID-19) and SARS-CoV-2 and suggested fenofibrate as a potential
therapy for COVID-19.
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Citations
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Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi
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Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
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- Antiviral activity of Poncirus trifoliata seed extract against oseltamivirresistant influenza virus
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Yoonki Heo , Yeondong Cho , Kwon sung Ju , Hansam Cho , Ki Hoon Park , Hanul Choi , Jong Kwang Yoon , Chiung Moon , Young Bong Kim
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J. Microbiol. 2018;56(8):586-592. Published online July 25, 2018
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DOI: https://doi.org/10.1007/s12275-018-8222-0
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1,125
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Abstract
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The emergence of oseltamivir-resistant variants of influenza
virus has highlighted the necessity for the development of
more effective novel antiviral drugs. To date, numerous researchers
have focused on developing antiviral drugs using
natural resources, such as traditional herbal medicines. Poncirus
trifoliata is widely used in oriental medicine as a remedy
for gastritis, dysentery, inflammation and digestive ulcers. In
this study, we investigated the potential antiviral effect of the
Poncirus trifoliata orange seed extract against influenza virus.
An ethanol extract of Poncirus trifoliata seeds (PTex) inhibited
the activity of influenza viruses, in particular, oseltamivir-
resistant strains, in Madin-Darby canine kidney cells. In
contrast to oseltamivir, PTex exerted a significant inhibitory
effect on the cellular penetration pathway of the virus rather
than HA receptor binding. The potent antiviral effect and novel
working mechanism of PTex support its further development
as an effective natural antiviral drug with a wide spectrum
of activity against influenza and oseltamivir-resistant
viruses.
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Citations
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