- Packaging of Porcine Reproductive and Respiratory Syndrome Virus Replicon RNA by a Stable Cell Line Expressing Its Nucleocapsid Protein
-
Byung-Hak Song , Jeong-Min Kim , Jin-Kyoung Kim , Han-Saem Jang , Gil-Nam Yun , Eun-Jin Choi , Jae-Young Song , Sang-Im Yun , Young-Min Lee
-
J. Microbiol. 2011;49(3):516-523. Published online June 30, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1280-1
-
-
787
View
-
2
Download
-
5
Crossref
-
 Abstract
 PDF
-
Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the Arteriviridae family, is one of the most common and economically important swine pathogens. Although both live-attenuated and killed-inactivated vaccines against the virus have been available for a decade, PRRSV is still a major problem in the swine industry worldwide. To explore the possibility of producing single-round infectious PRRSV replicon particles as a potential vaccine strategy, we have now generated two necessary components: 1) a stable cell line (BHK/Sinrep19/PRRSV-N) that constitutively expresses the viral nucleocapsid (N) protein localized to the cytoplasm and the nucleolus and 2) a PRRSV replicon vector (pBAC/PRRSV/Replicon-ΔN) with a 177-nucleotide deletion, removing the 3′-half portion of ORF7 in the viral genome, from which the self-replicating propagation-defective replicon RNAs were synthesized in vitro by SP6 polymerase run-off
transcription. Transfection of this replicon RNA into N protein-expressing BHK-21 cells led to the secretion of infectious particles that packaged the replicon RNA, albeit with a low production efficiency of 0.4×102 to 1.1×102 infectious units/ml; the produced particles had only single-round infectivity with no cell-to-cell spread. This trans-complementation system for PRRSV provides a useful platform for studies to define the packaging signals and motifs present within the viral genome and N protein, respectively, and to develop viral replicon-based antiviral vaccines that will stop the infection and spread of this pathogen.
-
Citations
Citations to this article as recorded by  - Research progress on the N protein of porcine reproductive and respiratory syndrome virus
Yajie Zheng, Gan Li, Qin Luo, Huiyang Sha, Hang Zhang, Ruining Wang, Weili Kong, Jiedan Liao, Mengmeng Zhao
Frontiers in Microbiology.2024;[Epub] CrossRef - Glycosylated protein 4-deficient PRRSV in complementing cell line shows low virus titer
Hao Guo, Wudong Gaowa, Hongzhe Zhao, Chunyu Liu, Lina Hou, Yongjun Wen, Fengxue Wang
Research in Veterinary Science.2023; 158: 84. CrossRef - Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications
Jayeshbhai Chaudhari, Hiep L. X. Vu
Viruses.2020; 12(11): 1245. CrossRef - Veterinary Replicon Vaccines
Mia C. Hikke, Gorben P. Pijlman
Annual Review of Animal Biosciences.2017; 5(1): 89. CrossRef - Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology
Long Liu, Zoe Lear, David J. Hughes, Weining Wu, En-min Zhou, Adrian Whitehouse, Hongying Chen, Julian A. Hiscox
Veterinary Microbiology.2015; 176(1-2): 109. CrossRef
|
MSK
