- Functional properties of the major outer membrane protein in Stenotrophomonas maltophilia
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Yih-Yuan Chen , Han-Chiang Wu , Juey-Wen Lin , Shu-Fen Weng
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J. Microbiol. 2015;53(8):535-543. Published online July 31, 2015
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DOI: https://doi.org/10.1007/s12275-015-5202-5
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 Abstract
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Stenotrophomonas maltophilia is an opportunistic pathogen
that is closely associated with high morbidity and mortality
in debilitated and immunocompromised individuals.
Therefore, to investigate the pathogenesis mechanism is urgently
required. However, there are very few studies to evaluate
the functional properties of outer membrane protein,
which may contribute to the pathogenesis in S. maltophilia.
In this study, three abundant proteins in the outer membrane
fraction of S. maltophilia were identified by liquid chromatography-
tandem mass spectrometry as OmpW1, MopB, and
a hypothetical protein. MopB, a member of the OmpA family,
was firstly chosen for functional investigation in this study
because many OmpA-family proteins are known to be involved
in pathogenesis and offer potential as vaccines. Membrane
fractionation analyses demonstrated that MopB was
indeed the most abundant outer membrane protein (OMP)
in S. maltophilia. For functional studies, the mopB mutant
of S. maltophilia (SmMopB) was constructed by insertional
mutation. MopB deficiency resulted in a change in the protein
composition of OMPs and altered the architecture of the
outer membrane. The SmMopB strain exhibited reduced
cytotoxicity toward L929 fibroblasts and was more sensitive
to numerous stresses, including human serum, sodium dodecyl
sulfate, and hydrogen peroxide compared with wildtype
S. maltophilia. These results suggest that MopB may be
a good candidate for the design of vaccines or anti-MopB
drugs for controlling serious nosocomial infections of multidrug-
resistant S. maltophilia, especially in immunosuppressed
patients.
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