- Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome
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Caixin Yang , Xingxing Lian , Yanpeng Cheng , Yifan Jiao , Jing Yang , Kui Dong , Shan Lu , Xin-He Lai , Dong Jin , Han Zheng , Ji Pu , Suping Wang , Liyun Liu , Jianguo Xu
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J. Microbiol. 2022;60(6):585-593. Published online April 18, 2022
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DOI: https://doi.org/10.1007/s12275-022-1673-3
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Two Gram-stain-positive, catalase-negative, non-spore-forming,
cocci-shaped strains (dk850T and JY899) were isolated
from the feces of Equus kiang in the Qinghai-Tibet Plateau of
China. 16S rRNA gene sequence-based phylogenetic analyses
showed that strains dk850T and JY899 belong to the genus
Flaviflexus, closest to F. salsibiostraticola KCTC 33148T, F. ciconiae
KCTC 49253T and F. huanghaiensis H5T. The DNA
G + C content of strain dk850T was 62.9%. The digital DNADNA
hybridization values of strain dk850T with the closely related
species were below the 70% threshold for species demarcation.
The two strains grew best at 28°C on brain heart infusion
(BHI) agar with 5% sheep blood. All strains had C18:1ω9c
and C16:0 as the major cellular fatty acids. MK-9(H4) was the
major menaquinone in strain dk850T. The major polar lipids
included diphosphatidylglycerol and an unidentified phospholipid.
Strains dk850T and JY899 were identified as carrying
a class 1 integron containing the aminoglycoside resistance
gene aadA11, both strains were resistant to spectinomycin
and streptomycin. Based on several lines of evidence
from phenotypic and phylogenetic analyses, strains dk850T
and JY899 represent a novel species of the genus Flaviflexus,
for which the name Flaviflexus equikiangi sp. nov. is proposed.
The type strain is dk850T (= CGMCC 1.16593T = JCM 33598T).
- Agromyces laixinhei sp. nov. isolated from bat feces in China
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Yanpeng Cheng , Yibo Bai , Yuyuan Huang , Jing Yang , Shan Lu , Dong Jin , Ji Pu , Han Zheng , Junqin Li , Ying Huang , Suping Wang , Jianguo Xu
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J. Microbiol. 2021;59(5):467-475. Published online March 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-0546-5
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1,078
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Abstract
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Three rod-shaped, Gram-stain-positive, and catalase-positive,
phenotypically closely related isolates (HY052T, HY050, and
HY045) were obtained from fecal samples collected from bats
in Guangxi province and Chongqing city of China. Circular,
smooth, light-yellow colonies appeared on brain heart infusion
plate after 24–48 h incubation at 28°C. The optimal pH
for growth was between 6.0 and 7.5. Based on 16S rRNA, the
three isolates were phylogenetically related to Agromyces terreus
DS-10T, Agromyces aureus AR33T, Agromyces salentinus
20-5T, Agromyces allii UMS-62T, Agromyces lapidis CD55T,
and Agromyces italicus CD1T. Moreover, based on 296 core
genes, the phylogenomic tree indicated that the three isolates
clustered together, closest to Agromyces cerinus VKM Ac-
1340T and Agromyces fucosus VKM Ac-1345T but separated
distantly from other Agromyces species. The average nucleotide
identity values between strain HY052T and other Agromyces
species ranged from 79.3% to 87.9%, lower than the
95–96% threshold. Furthermore, the genome of strain HY052T
contains a circular chromosome of 3,437,203 bp with G + C
content of 69.0 mol%. Main fatty acids were anteiso-C15:0
and anteiso-C17:0. The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, and unidentified glycolipids.
Rhamnose, ribose, and glucose were the primary cell wall sugars.
The major peptidoglycan amino acids included alanine,
glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional
remarkable difference from other Agromyces species
is that MK-12 was the sole menaquinone in strain HY052T.
Based on results from the polyphasic characterizations performed
in this study, our isolates are proposed to be members
of a novel species in genus Agromyces, named Agromyces
laixinhei. The type strain is HY052T (= CGMCC 1.17175T =
JCM 33695T).
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Citations
Citations to this article as recorded by  - Leucobacter faecis sp. nov., a Novel Actinomycete Isolated from Bat Feces of Rousettus and Hipposideros spp.
Yuyuan Huang, Jing Yang, Qinglian Qin, Zedan Yang, Xiaoting Wei, Ziwei Lin, Wenbo Luo, Wudi Wei, Zongxiang Yuan, Li Ye, Junjun Jiang, Shan Lu, Hao Liang
Current Microbiology.2026;[Epub] CrossRef - Aleya cavernae gen. nov., sp. nov., and Lacrimispora cavernae sp. nov., novel taxa isolated from bat guano
Samuel L. Miller, Nam Lu, Payton R. Shears, Jacobey King, Krithivasan Sankaranarayanan, Paul A. Lawson
International Journal of Systematic and Evolutionary Microbiology
.2026;[Epub] CrossRef - Validation List no. 223. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef -
Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata
Juan Guzman, Maite Ortúzar, Anja Poehlein, Rolf Daniel, Martha E. Trujillo, Andreas Vilcinskas
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - The Threat of Potentially Pathogenic Bacteria in the Feces of Bats
Yuyuan Huang, Yamin Sun, Qianni Huang, Xianglian Lv, Ji Pu, Wentao Zhu, Shan Lu, Dong Jin, Liyun Liu, Zhengli Shi, Jing Yang, Jianguo Xu, Biao He
Microbiology Spectrum.2022;[Epub] CrossRef - Morphological and genomic characteristics of two novel halotolerant actinomycetes, Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces
Yuyuan Huang, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Juan Zhou, Sihui Zhang, Qianni Huang, Xianglian Lv, Wentao Zhu, Ji Pu, Ying Huang, Liyun Liu, Jianguo Xu
Systematic and Applied Microbiology.2022; 45(2): 126294. CrossRef - Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef
- Vagococcus zengguangii sp. nov., isolated from yak faeces
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Yajun Ge , Dong Jin , Xin-He Lai , Jing Yang , Shan Lu , Ying Huang , Han Zheng , Xiaoyan Zhang , Jianguo Xu
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J. Microbiol. 2021;59(1):1-9. Published online December 23, 2020
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DOI: https://doi.org/10.1007/s12275-021-0406-3
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966
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Abstract
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Two unknown Gram-stain-positive, catalase- and oxidasenegative,
non-motile, and coccus-shaped bacteria, designated
MN-17T and MN-09, were isolated from yaks faeces (Bos
grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA
gene sequence-based comparative analyses revealed that the
two strains were grouped within the genus Vagococcus, displaying
the highest similarity with Vagococcus xieshaowenii
CGMCC 1.16436T (98.6%) and Vagococcus elongatus CCUG
51432T (96.4%). Both strains grew optimally at 37°C and pH
7.0 in the presence of 0.5% (w/v) NaCl. The complete genome
of MN-17T comprises 2,085 putative genes with a total
of 2,190,262 bp and an average G + C content of 36.7 mol%.
The major fatty acids were C16:0 (31.2%), C14:0 (28.5%), and
C18:1ω9c (13.0%); the predominant respiratory quinone was
MK-7 (68.8%); the peptidoglycan type was A4α(L-Lys-DAsp);
and the major polar lipid was diphosphatidylglycerol.
Together, these supported the affiliation of strain MN-17T
to the genus Vagococcus. In silico DNA-DNA hybridization
and the average nucleotide identity values between MN-17T
and all recognized species in the genus were 21.6–26.1%
and 70.7–83.0%, respectively. MN-17T produced acid from
D-cellobiose, D-fructose, glycerol, D-glucose, N-acetyl-glucosamine,
gentiobiose, D-mannose, D-maltose, D-ribose, Dsaccharose,
salicin, D-trehalose, and D-xylose. These results
distinguished MN-17T and MN-09 from closely related species
in Vagococcus. Thus, we propose that strains MN-17T
and MN-09 represent a novel species in the genus Vagococcus,
with the name Vagococcus zengguangii sp. The type strain
is MN-17T (= CGMCC 1.16726T = GDMCC 1.1589T = JCM
33478T).
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Citations
Citations to this article as recorded by - Leucobacter faecis sp. nov., a Novel Actinomycete Isolated from Bat Feces of Rousettus and Hipposideros spp.
Yuyuan Huang, Jing Yang, Qinglian Qin, Zedan Yang, Xiaoting Wei, Ziwei Lin, Wenbo Luo, Wudi Wei, Zongxiang Yuan, Li Ye, Junjun Jiang, Shan Lu, Hao Liang
Current Microbiology.2026;[Epub] CrossRef -
Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat
Per Johansson, Elina Jääskeläinen, Elina Säde, Johanna Björkroth
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef
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