- Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens
-
Md. Azizul Haque , Han Dae Yun , Kye Man Cho
-
J. Microbiol. 2016;54(5):353-363. Published online April 20, 2016
-
DOI: https://doi.org/10.1007/s12275-016-5641-7
-
-
995
View
-
0
Download
-
17
Crossref
-
 Abstract
 PDF
-
The study aimed to reveal the diversity of endophytic bacteria
in the roots of Chinese cabbage (CC) cultivated in two areas
in Korea, namely, Seosang-gun (SS) and Haenam-gun (HN),
and also in a transgenic plant (TP) from the laboratory. A
total of 653 colonies were isolated from the interior of CC
roots, comprising 118, 302, and 233 isolates from SS, HN,
and TP samples, respectively. Based on 16S rRNA gene sequence
analysis, the isolates belonged to four major phylogenetic
groups: high-G+C Gram-positive bacteria (HGC-GPB),
low-G+C Gram-positive bacteria (LGC-GPB), Proteobacteria,
and Bacteriodetes. The most dominant groups in the roots of
the SS, HN, and TP cultivars were LGC-GPB (48.3%), Proteobacteria
(50.2%), and HGC-GPB (38.2%), respectively.
Importantly, most of the isolates that produced cell-walldegrading
enzymes belonged to the genus Bacillus. Bacillus sp.
(HNR03, TPR06), Bacillus pumilus (SSR07, HNR11, TPR07),
and Bacillus subtilis (TPR03) showed high antagonism against
the tested food-borne pathogenic bacteria. In addition, Bacillus
sp. (HNR03, TPR06), Bacillus pumilus (SSR07, HNR11,
HNR17, TPR11), Microbacterium oxidans (SSR09, TPR04),
Bacillus cereus HNR10, Pseudomonas sp. HNR13, and Bacillus
subtilis (TPR02, TPR03) showed strong antagonistic activity
against the fungi Phythium ultimum, Phytophthora capsici,
Fusarium oxysporum, and Rhizoctonia solani. The endophytes
isolated from the TP cultivar showed the strongest antagonistic
reactions against pathogens. This study is the first report
on endophytic bacteria from Chinese cabbage roots.
-
Citations
Citations to this article as recorded by  - Tissue-specific assembly of endophytic microbiota in Agriophyllum squarrosum (L.) Moq.
Wenjing Li, Xin Xiang, Jiao Li, Wenlin Yang, Boai Jia, Wei Wang, Chengti Xu, Benyin Zhang, Hengxia Yin
BMC Microbiology.2026;[Epub] CrossRef - The role of bacterial endophytes in the therapeutic potential of medicinal plants: unveiling the mechanisms behind
Rajosik Bose, Ayan Samadder, Parvathy G. Nair, Sreya Dutta, Amit Kumar Dixit, Deepak Kumar, Shuvadip Sarkar, Kalyan Hazra, Shiddamallayya N. Mathapati, Sanjaya Kumar YR, Susmita Roy
Total Environment Microbiology.2026; 2(1): 100068. CrossRef - Antibiotic-resistant endophytic bacteria in food plants: phenotypic profiles and implications
Akshay Dhanokar, Amit Kumar, Priyanka Yadav, Siddaardha K Duggirala, Vineet Kumar, Pradeep Verma, Arvind Pratap Singh
Total Environment Microbiology.2026; 2(2): 100088. CrossRef - In silico modeling and in vitro evaluation of antidiabetic potential of exopolysaccharides from Brassicaceae root endophytic bacteria
Kusmiati Kusmiati, Arif Nurkanto, Asrul Fanani, Jendri Mamangkey, Dian Alfian Nurcahyanto, Khatarina Meldawati Pasaribu, Indriati Ramadhani, Ade Lia Putri, Fifi Afiati, Ni Wayan S. Agustini, Herman Irawan, Dody Priadi, Yelin Adalina, Muhammad Taufik, Silv
Journal of Genetic Engineering and Biotechnology.2026; 24(3): 100790. CrossRef - Clubroot (Plasmodiophora brassicae) Suppression Under Biocontrol Agents in Pak choi with Variations in Physiological, Biochemical, and Bacterial Diversity
Shazma Gulzar, Fiza Liaquat, Muhammad Khalid Hameed, Asad Rehman, Muhammad Salman Zahid, Muhammad Aamir Manzoor, Iftikhar Hussain Shah, Yidong Zhang
Journal of Plant Growth Regulation.2024; 43(5): 1331. CrossRef - Enhanced rice plant (BRRI-28) growth at lower doses of urea caused by diazinon mineralizing endophytic bacterial consortia and explorations of relevant regulatory genes in a Klebsiella sp. strain HSTU-F2D4R
Md. Azizul Haque, Simo, Md. Yeasin Prodhan, Sibdas Ghosh, Md. Shohorab Hossain, Aminur Rahman, Uttam Kumar Sarker, Md. Atiqul Haque
Archives of Microbiology.2023;[Epub] CrossRef - Problems and prospects of utilization of bacterial endophytes for the management of plant diseases
Dhruva Kumar Jha
Indian Phytopathology.2023; 76(1): 3. CrossRef - An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells
Mari Kurokawa, Masataka Nakano, Nobutaka Kitahata, Kazuyuki Kuchitsu, Toshiki Furuya
Scientific Reports.2021;[Epub] CrossRef - The hidden treasures of citrus: finding Huanglongbing cure where it was lost
Shahzad Munir, Ayesha Ahmed, Yongmei Li, Pengbo He, Brajesh K. Singh, Pengfei He, Xingyu Li, Suhail Asad, Yixin Wu, Yueqiu He
Critical Reviews in Biotechnology.2021; : 1. CrossRef -
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots
José David Flores-Félix, Esther Menéndez, Martha Helena Ramírez-Bahena, Alvaro Peix, Paula García-Fraile, Encarna Velázquez
International Journal of Systematic and Evolutionary Microbiology
.2020; 70(10): 5512. CrossRef - Physiological change alters endophytic bacterial community in clubroot of tumorous stem mustard infected by Plasmodiophora brassicae
Diandong Wang, Tingting Sun, Songyu Zhao, Limei Pan, Hongfang Liu, Xueliang Tian
BMC Microbiology.2020;[Epub] CrossRef - Unlocking the potential of plant growth-promoting rhizobacteria on soil health and the sustainability of agricultural systems
Zobia Khatoon, Suiliang Huang, Mazhar Rafique, Ali Fakhar, Muhammad Aqeel Kamran, Gustavo Santoyo
Journal of Environmental Management.2020; 273: 111118. CrossRef - Origin of lactic acid bacteria in mulkimchi fermentation
Chung Eun Hwang, Md. Azizul Haque, Su Young Hong, Su Cheol Kim, Kye Man Cho
Journal of Applied Biological Chemistry.2019; 62(4): 441. CrossRef - Isolation and diversity of culturable rhizobacteria associated with economically important crops and uncultivated plants in Québec, Canada
Di Fan, Timothy Schwinghamer, Donald L. Smith
Systematic and Applied Microbiology.2018; 41(6): 629. CrossRef - Biocontrol of the internalization of Salmonella enterica and Enterohaemorrhagic Escherichia coli in mung bean sprouts with an endophytic Bacillus subtilis
Zhenyu Shen, Azlin Mustapha, Mengshi Lin, Guolu Zheng
International Journal of Food Microbiology.2017; 250: 37. CrossRef - Symbiosis-inspired approaches to antibiotic discovery
Navid Adnani, Scott R. Rajski, Tim S. Bugni
Natural Product Reports.2017; 34(7): 784. CrossRef - A endophytic fungus, Ramichloridium cerophilum, promotes growth of a non-mycorrhizal plant, Chinese cabbage
Xie Ling, Usui Erika, Narisawa Kazuhiko
African Journal of Biotechnology.2016; 15(25): 1299. CrossRef
- Chitinibacter suncheonensis sp. nov., a Chitinolytic Bacterium from a Mud Flat in Suncheon Bay
-
Sung Kyum Kim , Yong Ho Kim , Yu Seok Jeong , Han Beur Na , Jungho Kim , Keun Sik Baik , Han Dae Yun , Jung-Kul Lee , Hoon Kim
-
J. Microbiol. 2012;50(6):1058-1062. Published online December 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2333-9
-
-
707
View
-
0
Download
-
6
Crossref
-
Abstract
PDF
-
A chitinolytic bacterium, designated strain SK16T, was isolated from a mud flat in Suncheon Bay, Republic of Korea. Strain SK16T is Gram-negative, strictly aerobic, motile by a polar flagellum, and short rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Chitinibacter and was most closely related to Chitinibacter tainanensis S1T (98.2% similarity). DNA-DNA hybridization analyses showed a low association value of 20.45±4.08% between them. The major cellular fatty acids, the G+C content of the genomic DNA, and the predominant quinone of the strain were summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c; 50.5%) and C12:0 (12.5%), 52.26 mol%, and Q-8, respectively. Based on the phylogenetic, chemotaxonomic, and phenotypic properties, strain SK16T represents a novel species of the genus Chitinibacter, for which the name Chitinibacter suncheonensis sp. nov. is proposed. The type strain is SK16T (=KCTC 23839T =DSM 25421T).
-
Citations
Citations to this article as recorded by - Characterization of a novel Bi-functional cold-adapted chitinase from Chitinibacter mangrovi FCG-7T for efficient production of N-acetyl-D-glucosamine
Chenxi Gu, Liyan Yang, Susu Han, Dengfeng Yang, Xiaodong Xu, WenChao Zhang, Mingguo Jiang, Lixia Pan
Food Bioscience.2026; 75: 108168. CrossRef - Impact of moisture on microbial decomposition phenotypes and enzyme dynamics
Nicholas J Reichart, Sheryl Bell, Vanessa A Garayburu-Caruso, Natalie Sadler, Sharon Zhao, Kirsten S Hofmockel
The ISME Journal.2025;[Epub] CrossRef - Validation List no. 226: valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef - Molecular Characterization of Four Alkaline Chitinases from Three Chitinolytic Bacteria Isolated from a Mudflat
Sung Kyum Kim, Jong Eun Park, Jong Min Oh, Hoon Kim
International Journal of Molecular Sciences.2021; 22(23): 12822. CrossRef - The Draft Genome Sequence and Analysis of an Efficiently Chitinolytic Bacterium Chitinibacter sp. Strain GC72
Alei Zhang, Xiaofang Mo, Guoguang Wei, Ning Zhou, Sai Yang, Jie Chen, Yingying Wang, Kequan Chen, Pingkai Ouyang
Current Microbiology.2020; 77(12): 3903. CrossRef - Characterization of extracellular chitinase from Chitinibacter sp. GC72 and its application in GlcNAc production from crayfish shell enzymatic degradation
Cong Gao, Alei Zhang, Kequan Chen, Zhikui Hao, Junmao Tong, Pingkai Ouyang
Biochemical Engineering Journal.2015; 97: 59. CrossRef
- The Role of Carbohydrate-Binding Module (CBM) Repeat of a Multimodular Xylanase (XynX) from Clostridium thermocellum in Cellulose and Xylan Binding
-
Thangaswamy Selvaraj , Sung Kyum Kim , Yong Ho Kim , Yu Seok Jeong , Yu-Jeong Kim , Nguyen Dinh Phuong , Kyung Hwa Jung , Jungho Kim , Han Dae Yun , Hoon Kim
-
J. Microbiol. 2010;48(6):856-861. Published online January 9, 2011
-
DOI: https://doi.org/10.1007/s12275-010-0285-5
-
-
812
View
-
0
Download
-
18
Crossref
-
Abstract
PDF
-
A non-cellulosomal xylanase from Clostridium thermocellum, XynX, consists of a family-22 carbohydratebinding module (CBM22), a family-10 glycoside hydrolase (GH10) catalytic module, two family-9 carbohydrate-binding modules (CBM9-I and CBM9-II), and an S-layer homology (SLH) module. E. coli BL21(DE3) (pKM29), a transformant carrying xynX', produced several truncated forms of the enzyme.
Among them, three major active species were purified by SDS-PAGE, activity staining, gel-slicing, and diffusion from the gel. The truncated xylanases were different from each other only in their C-terminal regions. In addition to the CBM22 and GH10 catalytic modules, XynX1 had the CBM9-I and most of the CBM9-II, XynX2 had the CBM9-I and about 40% of the CBM9-II, and XynX3 had about 75% of the CBM9-I. The truncated xylanases showed higher binding capacities toward Avicel than those toward insoluble xylan. XynX1 showed a higher affinity toward Avicel (70.5%) than XynX2 (46.0%) and XynX3 (42.1%); however, there were no significant differences in the affinities toward insoluble xylan. It is suggested that the CBM9 repeat, especially CBM9-II, of XynX plays a role in xylan degradation in nature by strengthening cellulose binding rather than by enhancing xylan binding.
-
Citations
Citations to this article as recorded by - Xylanases from Cellulomonas sp. B6 Produce Xylooligosaccharides with Prebiotic Potential from Agro-Industrial Byproducts
Ornella Mailén Ontañon, Hebe Mónica Dionisi, Juliana Topalian, Laura Emilce Navas, Caio Cesar de Mello Capetti, María Esperanza Cerón Cucchi, Victoria Mariel Hracek, Laura Faas, Adam Dowle, Igor Polikarpov, Marina Ciancia, Leonardo Dario Gómez, Eleonora C
Journal of Agricultural and Food Chemistry.2026;[Epub] CrossRef - Eggshell-zeolite composite additive enhances humification and mitigates CH4 emissions during cow-manure composting: Insights from metagenomic analysis
Hongbo Zhang, Jinbiao Gao, Haolong Wu, Yufeng Chen, Xiang He, Xueqin He
Environmental Technology & Innovation.2026; 43: 105058. CrossRef - Structure-Informed Insights into Catalytic Mechanism and Multidomain Collaboration in α-Agarase CmAga
Yuxian You, Bee Koon Gan, Min Luo, Xinzhe Zheng, Nanqing Dong, Yixiong Tian, Caiming Li, Haocun Kong, Zhengbiao Gu, Daiwen Yang, Zhaofeng Li
Journal of Agricultural and Food Chemistry.2025; 73(13): 7975. CrossRef - Cellulolytic and hemicellulolytic capacity of Acetivibrio clariflavus
Katarína Šuchová, Vladimír Puchart
Applied Microbiology and Biotechnology.2025;[Epub] CrossRef - Characterization of the thermophilic xylanase Fsa02490Xyn from the hyperthermophile Fervidibacter sacchari belonging to glycoside hydrolase family 10
Nicole Torosian, Jonathan K. Covington, Allison M. Cook, Nancy O. Nou, Marike Palmer, Ritesh Mewalal, Miranda Harmon‐Smith, Ian K. Blaby, Jan‐Fang Cheng, Matthias Hess, Brian P. Hedlund
FEBS Open Bio.2025; 15(10): 1629. CrossRef - Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion
Min Xiao, Ya-Jun Liu, Edward A. Bayer, Akihiko Kosugi, Qiu Cui, Yingang Feng
Green Carbon.2024; 2(1): 57. CrossRef - Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces
Wenjing Lu, Biqing Zha, Jie Lyu, Chenxi LingHu, Jing Chen, Sisi Deng, Xiangling Zhang, Liang Li, Guoqing Wang
Frontiers in Microbiology.2024;[Epub] CrossRef - Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes
Yuxian You, Haocun Kong, Caiming Li, Zhengbiao Gu, Xiaofeng Ban, Zhaofeng Li
Biotechnology Advances.2024; 73: 108365. CrossRef - Xylan-breakdown apparatus of Clostridium thermocellum
Pedro R. V. Hamann, Eliane F. Noronha
Cellulose.2022; 29(14): 7535. CrossRef - Exploration of the key functional proteins from an efficient cellulolytic microbial consortium using dilution-to-extinction approach
Qinghua Zhang, Hanguang Li, Xiangdong Zhu, Fenju Lai, Zhijun Zhai, Yuanxiu Wang
Journal of Environmental Sciences.2016; 43: 199. CrossRef - Biochemical characterization of a novel thermostable GH11 xylanase with CBM6 domain from Caldicellulosiruptor kronotskyensis
Weibo Qiao, Shuge Tang, Shuofu Mi, Xiaojing Jia, Xiaowei Peng, Yejun Han
Journal of Molecular Catalysis B: Enzymatic.2014; 107: 8. CrossRef - Thermophilic lignocellulose deconstruction
Sara E. Blumer-Schuette, Steven D. Brown, Kyle B. Sander, Edward A. Bayer, Irina Kataeva, Jeffrey V. Zurawski, Jonathan M. Conway, Michael W. W. Adams, Robert M. Kelly
FEMS Microbiology Reviews.2014; 38(3): 393. CrossRef - An evolutionary analysis of the GH57 amylopullulanases based on the DOMON_glucodextranase_like domains
Yu‐Liang Jiao, Shu‐Jun Wang, Ming‐Sheng Lv, Yao‐Wei Fang, Shu Liu
Journal of Basic Microbiology.2013; 53(3): 231. CrossRef - Purification, Characterization of GH11 Endo-β-1,4-xylanase from Thermotolerant Streptomyces sp. SWU10 and Overexpression in Pichia pastoris KM71H
Warin Deesukon, Yuichi Nishimura, Tatsuji Sakamoto, Wasana Sukhumsirichart
Molecular Biotechnology.2013; 54(1): 37. CrossRef - Recombinant production and characterization of full-length and truncated β-1,3-glucanase PglA from Paenibacillussp. S09
Rui Cheng, Jinping Chen, Xiaohong Yu, Yang Wang, Shiming Wang, Jianfa Zhang
BMC Biotechnology.2013;[Epub] CrossRef - Production of XynX, a Large Multimodular Protein of Clostridium thermocellum, by Protease-Deficient Bacillus subtilis Strains
Nguyen Dinh Phuong, Yu Seok Jeong, Thangaswamy Selvaraj, Sung Kyum Kim, Yong Ho Kim, Kyung Hwa Jung, Jungho Kim, Han Dae Yun, Sui-Lam Wong, Jung-Kul Lee, Hoon Kim
Applied Biochemistry and Biotechnology.2012; 168(2): 375. CrossRef - Biochemical and Structural Insights into Xylan Utilization by the Thermophilic Bacterium Caldanaerobius polysaccharolyticus
Yejun Han, Vinayak Agarwal, Dylan Dodd, Jason Kim, Brian Bae, Roderick I. Mackie, Satish K. Nair, Isaac K.O. Cann
Journal of Biological Chemistry.2012; 287(42): 34946. CrossRef - A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity
Marie Couturier, Julia Feliu, Mireille Haon, David Navarro, Laurence Lesage-Meessen, Pedro M Coutinho, Jean-Guy Berrin
Microbial Cell Factories.2011;[Epub] CrossRef
|
MSK
