Journal of Microbiology

Hak Kyu Yi

1 Article

Characterization of Bacillus cereus SH-7 Extracellular Protease: Hak Kyu Yi , Young Jin Chum , Han Bok Kim; J. Microbiol. 1999;37(4):213-217.

Abstract PDF: An extracellular endopeptidase from Bacillus cereus SH-7 was purified to homogeneity. The protease was most active at pH 8 and 40 C, respectively. The molecular mass of the protease was 40 kDa on SDS-PAGE, and 120 kDa by gel filtration, suggesting that the native enzyme is composed of three homogeneous subunits. The K_m and V_max values of the protease for N-succinyl-(Ala)_2-Pro-Phe-p-nitroanilide were 11.11 mM and 170 nmol/mg of protein/min, respectively. The protease was also identified as a metalloprotease. The bioactivity of the SH-7 protease will need further study in the future.

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