Journal of Microbiology

Hae Kyung Lee

4 Articles

Diagnosis and molecular characteristics of human infections caused by Anaplasma phagocytophilum in South Korea: Seung Hun Lee , Sungdo Park , Yeong Seon Lee , Hae Kyung Lee , Seon Do Hwang; J. Microbiol. 2018;56(11):847-853. Published online October 24, 2018; DOI: https://doi.org/10.1007/s12275-018-8385-8

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Human granulocytic anaplasmosis (HGA) is a tick borne infection caused by Anaplasma phagocytophilum. HGA cases in South Korea have been identified since the first report in 2014. In this study, we investigated the serological response in 594 clinical samples of patients with acute febrile illness and molecular characteristics of A. phagocytophilum clinical isolates obtained from HGA patients. In serological test for A. phagocytophilum, 7.91% (47/594 cases) were positive for IgG and Ig M and 13 of 47 cases showed seroconversion. In the detection rate of the 16S rRNA, msp2(p44), and ankA, genes were showed 3.68% (14/380 cases) for A. phagocytophilum- specific 16S rRNA gene. Phylogenetic analysis of three clinical isolates demonstrated high sequence similarity (98.58– 100%) with A. phagocytophilum 16S rRNA sequences identified from public databases. Analysis of the msp2(p44) gene showed highly variable similarity rates (7.24–98.85%) even within isolated countries and host ranges. These results provide clues into the bacterial characterization of A. phagocytophilum originating from Korean patients, providing useful guidance for treatment and improving clinical outcomes.

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Human granulocytotropic anaplasmosis—A systematic review and analysis of the literature
Sophie Schudel, Larissa Gygax, Christian Kositz, Esther Kuenzli, Andreas Neumayr, Ana LTO Nascimento
PLOS Neglected Tropical Diseases.2024; 18(8): e0012313. CrossRef
Molecular and serological detection of Anaplasma spp. in small ruminants in an area of Cerrado Biome in northeastern Brazil
Ellainy Maria Conceição Silva, Ingrid Carolinne Lopes Marques, Victória Valente Califre de Mello, Renan Bressianini do Amaral, Luiz Ricardo Gonçalves, Maria do Socorro Costa Oliveira Braga, Larissa Sarmento dos Santos Ribeiro, Rosangela Zacarias Machado,
Ticks and Tick-borne Diseases.2024; 15(1): 102254. CrossRef
Global status of Anaplasma phagocytophilum infections in human population: A 50-year (1970–2020) meta-analysis
Solomon Ngutor Karshima, Musa Isiyaku Ahmed, Kaltume Mamman Mohammed, Victoria Adamu Pam
Journal of Vector Borne Diseases.2023; 60(3): 265. CrossRef
Human granulocytic anaplasmosis in a Single University Hospital in the Republic of Korea
Da Young Kim, Jun-Won Seo, Na Ra Yun, Choon-Mee Kim, Dong-Min Kim
Scientific Reports.2021;[Epub] CrossRef
Development of a duplex PCR assay for detecting Theileria luwenshuni and Anaplasma phagocytophilum in sheep and goats
Yaqun Yan, Yanyan Cui, Shanshan Zhao, Jichun Jing, Ke Shi, Fuchun Jian, Longxian Zhang, Rongjun Wang, Kunlun Wang, Yongchun Zhou, Changshen Ning
Experimental and Applied Acarology.2021; 85(2-4): 319. CrossRef
The Novel Zoonotic Pathogen, Anaplasma capra, Infects Human Erythrocytes, HL-60, and TF-1 Cells In Vitro
Yongshuai Peng, Chenyang Lu, Yaqun Yan, Jinxing Song, Zhiyang Pei, Pihong Gong, Rongjun Wang, Longxian Zhang, Fuchun Jian, Changshen Ning
Pathogens.2021; 10(5): 600. CrossRef
Parasitic and Vector-Borne Infections in HIV-Positive Patients in Slovakia—Evidence of an Unexpectedly High Occurrence of Anaplasma phagocytophilum
Katarína Šimeková, Ľubomír Soják, Bronislava Víchová, Lenka Balogová, Júlia Jarošová, Daniela Antolová
Pathogens.2021; 10(12): 1557. CrossRef
Are other tick-borne infections overlooked in patients investigated for Lyme neuroborreliosis? A large retrospective study from South-eastern Sweden
Paula Gyllemark, Peter Wilhelmsson, Camilla Elm, Dieuwertje Hoornstra, Joppe W. Hovius, Marcus Johansson, Ivar Tjernberg, Per-Eric Lindgren, Anna J. Henningsson, Johanna Sjöwall
Ticks and Tick-borne Diseases.2021; 12(5): 101759. CrossRef
A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
Yongshuai Peng, Shanshan Zhao, Kunlun Wang, Jinxing Song, Yaqun Yan, Yongchun Zhou, Ke Shi, Fuchun Jian, Rongjun Wang, Longxian Zhang, Changshen Ning
Frontiers in Microbiology.2020;[Epub] CrossRef
Human granulocytic anaplasmosis in Kinmen, an offshore island of Taiwan
Kun-Hsien Tsai, Lo-Hsuan Chung, Chia-Hao Chien, Yu-Jung Tung, Hsin-Yi Wei, Tsai-Ying Yen, Pei-Yun Shu, Hsi-Chieh Wang, José Reck
PLOS Neglected Tropical Diseases.2019; 13(9): e0007728. CrossRef
Co-Infection of Scrub Typhus and Human Granulocytic Anaplasmosis in Korea, 2006
Jeong-Han Kim, Chang-Seop Lee, Chisook Moon, Yee Gyung Kwak, Baek-Nam Kim, Eu Suk Kim, Jae Myung Kang, Wan Beom Park, Myoung-don Oh, Sang-Won Park
Journal of Korean Medical Science.2019;[Epub] CrossRef

Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection: Hwajung Yi , Mi-Seon Lee , Joo-Yeon Lee , Hae Kyung Lee , Chun Kang; J. Microbiol. 2015;53(2):166-175. Published online January 28, 2015; DOI: https://doi.org/10.1007/s12275-015-4642-2

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Since the 2009 pandemic, monoclonal antibodies (mAbs) for rapid influenza diagnostic tests (RIDT) have been developed for specific diagnostics of pandemic viral infection. Most of the mAbs were poorly characterized because of urgency during the pandemic. Further characterization of the mAbs for RIDTs would be beneficial for understanding the immunological properties of the pandemic virus and utilizing the mAbs for other research purposes. In this study, it was confirmed that two mAbs (I38 and D383) in an RIDT for H1N1pdm09 diagnostics were able to detect H1N1pdm09 virus through enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA). Also, the two mAbs exhibited reactivity to hemagglutinins (HAs) of both the H1N1pdm09 and 1918 H1N1 viruses; therefore, the RIDT using the mAbs could detect HAs of H1N1pdm09 and also HAs of 1918 H1N1-like strains. In an extension to our previous study, the epitopes (Sa antigenic site and the interface area of F?and vestigial esterase subdomains on the HA1 domain of HA of H1N1pdm09) recognized by the mAbs were corroborated in depth by IFA with escape-mutants from the mAbs and mapping of the epitopes on the crystal structure of human H1N1 viral HAs. Collectively, these results imply that the mAbs for the RIDT may be suitable for use in studying the immunological properties of H1N1pdm09 viruses and that the Sa antigenic site and the interface area between F?and vestigial esterase subdomains on influenza viral HA recognized by the mAbs are immunologically conserved regions between H1N1pdm09 and 1918 H1N1.

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Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia
Ayako Ohkawara, Masatoshi Okamatsu, Makoto Ozawa, Duc‐Huy Chu, Lam Thanh Nguyen, Takahiro Hiono, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda
Microbiology and Immunology.2017; 61(5): 149. CrossRef

Genomic Diversity of Legionella pneumophila Serogroup 1 from Environmental Water Sources and Clinical Specimens Using Pulsed-Field Gel Electrophoresis (PFGE) from 1985 to 2007, Korea: Hae Kyung Lee , Yeon Ho Kang , Jae Yon Yu; J. Microbiol. 2010;48(5):547-553. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0031-z

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The molecular typing of 202 Legionella pneumophila sg 1 isolates obtained from environmental water sources and clinical specimens from 1985 to 2007 was conducted using pulsed-field gel electrophoresis (PFGE). In this study, a total of 212 isolates were grouped into 35 different PFGE types and Type 1 was the predominant type, accounting for 28.7% in PFGE types. Type 1 and Type 8 were observed continuously from 1985 to 2007. In the analysis of the distribution of PFGE types in six geographic regions (Seoul-Incheon, Gangwon, Chungcheong, Gyeongsang, Jeolla, and Jeju), Type 1 was predominant throughout four regions except for Jeolla and Jeju, and Type 6 was observed in four regions except two regions (Gangwon and Jeju). Six clinical isolates belonged to PFGE Type 1, Type 6, Type 9, and Type 15. Type 1 among these types, was isolated from 3 patients with confirmed nosocomial infection at the hospital and Type 6, Type 9, and Type 15 were isolated 3 patients with suspected community-acquired infection. Type R, PFGE pattern of L. pneumophila sg 1 (ATCC 33152, Philadelphia-1), was not observed in the isolates evaluated in this study. Therefore, our results suggest that PFGE Type 1 was very prevalent in the environmental and clinical isolates in Korea. Type 1 was distributed continuously for many years throughout Korea.

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Characterization of Legionella Isolated from the Water System at Public Facilities in Chungcheongnam-do Province
Younghee Cheon, Hyunah Lee, Hae-Sung Nam, Jihye Choi, Dayeon Lee, Young-Eun Ko, Jongjin Park, Miyoung Lee, Junhyuk Park
Journal of Environmental Health Sciences.2021; 47(5): 472. CrossRef
Characterization of Legionella pneumophila Populations by Multilocus Variable Number of Tandem Repeats (MLVA) Genotyping from Drinking Water and Biofilm in Hospitals from Different Regions of the West Bank
Ashraf R. Zayed, Marina Pecellin, Alaa Salah, Hanna Alalam, Suha Butmeh, Michael Steinert, Rene Lesnik, Ingrid Brettar, Manfred G. Höfle, Dina M. Bitar
Pathogens.2020; 9(11): 862. CrossRef
Quantitative Microbial Risk Assessment on Legionella Infection in Children Playing in Interactive Fountains
In-Sup Lee, Young-Gun Zo
The Korean Journal of Microbiology.2013; 49(4): 360. CrossRef
Protective immunity against Legionnaires' disease in an A/J mouse model using a DNA vaccine composed of an outer membrane protein (29 kDa) and the PilE fusion protein
Jia-nan Xu, Zhi-wei Yang, Jian-ping Chen, Da-Li Chen, Tao Wang, Ming-jie Liu, Lei Zhang, Bin-bin Yang
Diagnostic Microbiology and Infectious Disease.2012; 73(1): 9. CrossRef

Prevalence of Antibodies in Response to Legionella Species, Analysis of a Healthy Population from Jeollanam-do Province, Korea: Hae Kyung Lee , Mi Kyeong Woo , Yong In Ju , Soo Jin Baek , Hyeon Je Song , Jin Su Choi , Sun Seog Kweon , Doo Young Jeon , Yeon Ho Kang; J. Microbiol. 2008;46(2):160-164. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-007-0181-9

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Seroepidemological investigation of antibodies to Legionella species in 500 healthy individuals from a single geographical location in Korea was conducted by indirect fluorescent antibody assay (IFA). Considering an antibody titer of ≥1:128 as positive reaction, 15.2% of total sera were positive. In males and females older than 40 years old, levels of IgM and IgG were 1.2% and 14%, respectively. The sera with antibody titers of ≥1:128 to Legionella species accounted for 85 sera, and 9 sera of these were reacted to more than one Legionella species. Reactivity to L. bozemanii, L. micdadei, L. longbeachae, L. pneumophila sg 6, and L. gormanii were 32.9%, 20%, 15%, 10.6%, and 8%, respectively. However, L. pneumophila sg 1, sg 2, and sg 3 did not react to any sera. Serological analysis revealed that the level of antibody in response to L. bozemanii was more prevalent than L. pneumophila. Our results suggest that the antibodies of non-L. pneumophila species, such as L. bozemanii, may be highly prevalent in healthy population within Korea. Although conclusions based on the findings of this study must be cautiously considered given that the population sampled were sourced from a single province, we have added to the knowledge base of serodiagnosis of infections due to non-L. pneumophila species in Korea.

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The Korean Journal of Microbiology.2013; 49(4): 360. CrossRef
An Epidemiological Survey on Serological Diagnosis ofLegionnellaInfection in Seoul, Korea
Yong-Tae Yoon, Chang-Ho Han, Sung-Sun Choi, Jung-Mi Lee, Chang-Kyu Kim, Sung-Min Choi
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