- Kurthia ruminicola sp. nov., isolated from the rumen contents of a Holstein cow
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Myung Kyum Kim , Eun Tae Kim , Sang Bum Kim , Ha Yeon Jeong , Beom Young Park , Sathiyaraj Srinivasan
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J. Microbiol. 2018;56(1):36-41. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7285-2
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Gram-staining-positive, motile, rod-shaped bacteria, designated
as H31022T and H31024 was isolated from rumen contents
of a Holstein cow. Optimum growth occurred at 25°C
and pH 7.0 on R2A agar medium. Oxidase and catalase activities
are positive. The 16S rRNA gene sequence (1,452 bp)
of the new isolates revealed they belong to the genus Kurthia
of the phylum Firmicutes. Highest gene sequence similarities
were assessed to be with Kurthia massiliensis JC30T (98.4%),
Kurthia senegalensis JC8ET (97.5%), and Kurthia populi 10y-
14T (97.4%). Kurthia sibirica DSM 4747T (97.3%), Kurthia
zopfii NBRC 101529T (97.0%), and Kurthia gibsonii NCIMB
9758T (96.7%). DNA G + C content of strains H31022T and
H31024 were 34.4% and 39.7%. Strains H31022T and H31024
has the following chemotaxonomic characteristics; the major
fatty acids are iso-C15:0, iso-C14:0 and anteiso-C15; polar
lipid profile contained diphosphatidylglycerol (DPG), phosphatidylethanolamine
(PE), unknown aminophospholipids
(APL), unknown glycolipids (GL), unknown phospholipids
(PL), and unknown polar lipids (L); the major quinone is MK-7.
Based on polyphasic taxonomic analysis, strains H31022T
(= KCTC 33923T = JCM 19640T) and H31024 (= KCTC 33924T
= JCM 19641T) identified a novel species in the genus Kurthia
for which the name Kurthia ruminicola sp. nov. is proposed.
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Citations
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- Induction of Cytokines and Nitric Oxide in Murine Macrophages Stimulated with Enzymatically Digested Lactobacillus Strains
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Dong Woon Kim , Sung Back Cho , Cheol Heui Yun , Ha Yeon Jeong , Wan Tae Chung , Chang Weon Choi , Hyun Jeong Lee , In Sik Nam , Guk Hyun Suh , Sang Suk Lee , Byong Seak Lee
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J. Microbiol. 2007;45(5):373-378.
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DOI: https://doi.org/2601 [pii]
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Abstract
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Based on observations that lactic acid bacteria have the ability to activate macrophages, we assessed the potential effects of eight different Lactobacillus strains treated with gastrointestinal enzymes on the production of nitric oxide and various cytokines in macrophages. RAW 264.7 murine macrophage cells were cultured with either precipitates or supernatants of Lactobacillus strains digested with pepsin followed by pancreatin. The increased production of nitric oxide and interleukin (IL)-1β, IL-6, IL-12 and tumour necrosis factor (TNF)-α were observed when cultured with precipitates, and this effect was largely strain-dependent. In contrast, the exposure of RAW 264.7 cells to supernatants produced weaker or nearly undetectable effects in comparison to the effects of exposure to precipitates. The induction of nitric oxide appeared to be unaffected. These results demonstrate that nitric oxide and cytokines were effectively induced when the bacterial precipitate was treated with macrophages. The results of the present study also indicate that Lactobacillus strains treated with digestive enzymes are capable of stimulating the production of nitric oxide and cytokines in macrophages, which may modulate the gastrointestinal immune function of the host when it is given as a feed additive.
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