Journal of Microbiology

Gyu-Cheol Lee

3 Articles

Full Sequence Analysis and Characterization of a Human Astrovirus Type 1 Isolate from South Korea: Sung-Geun Lee , Lae-Hyung Kang , Weon-Hwa Jheong , Mi-Hwa Oh , Gyu-Cheol Lee , Sujeong Park , Soon-Young Paik; J. Microbiol. 2013;51(1):123-129. Published online March 2, 2013; DOI: https://doi.org/10.1007/s12275-013-2505-2

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Human astroviruses are recognized as an important cause of infantile gastroenteritis around the world. In South Korea, sporadic cases of HAstV infection have been reported since 2002. However, hitherto, there have been no studies reporting the whole genome sequence of an HAstV isolate from South Korea. Hence, we sequenced and analyzed the entire genome of an HAstV-1 strain (lhar) that was isolated in Seoul, South Korea. The whole-genome sequence analysis revealed 3 open reading frames comprising the whole genome: ORF1a (2,763 bp), ORF1b (1,548 bp), and ORF2 (2,364 bp). The lhar strain showed amino acid identities with 8 other reference strains of 87.6–98.7%, 94.2–98.8%, and 62.6–99.0% in the ORF1a, ORF1b, and ORF2 regions, respectively. The amino acid sequence of the capsid region encoded by ORF2 was compared with a total of 19 HAstV-1 strains and 8 HAstVs reference strains isolated in various countries. This revealed 1 amino acid substitution, at aa412 (Pro → Arg) in ORF2. This study, the first to report the fulllength sequence of an HAstV isolated in South Korea, is meaningful in that it can be used as a full-length HAstV sequence standard for future comparison studies. It may also prove useful to the field of public health field by facilitating the diagnosis and the prediction of new emerging variants.

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Full Sequence Analysis and Characterization of Human Bocavirus Type 2 in South Korea
Bomina Paik, Sung-Geun Lee , Han-Gil Cho, Yu-Jung Won, Lae-Hyung Kang, Soon-Young Paik, Seung-Jin Hong
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Complete genome sequencing of a recombinant strain between human astrovirus antigen types 2 and 8 isolated from South Korea
Hyun-Ju Ha, Sung-Geun Lee, Han-Gil Cho, Ji-Young Jin, Jae Woong Lee, Soon-Yong Paik
Infection, Genetics and Evolution.2016; 39: 127. CrossRef

Measurement of Antiviral Activities Using Recombinant Human Cytomegalovirus: Byung-Hak Song , Gyu-Cheol Lee , Chan-Hee Lee; J. Microbiol. 2000;38(4):255-259.

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Abstract PDF: For rapid and sensitive measurement of antiviral activities, application of a recombinant virus containing firefly luciferase gene was attempted. Recombinant human cytomegalovirus (HCMV) containing luciferase gene driven by HCMV late gene pp28 promoter (HCMV/pp28-luc) was used to test the antiviral activities of three known compounds and the result was compared with results from the conventional plaque assay for measuring the production of infectious viruses. When human fibroblast cells were infected with HCMV/pp28-luc, luciferase activity was observed at 2 days after infection and reached maximum at 6 days after infection, whereas the production of infectious virus was maximal at 4 days after infection. The antiviral activities of ganciclovir, acyclovir, and papaverine were measured in HFF cells infected with HCMV/pp28-luc and the luciferase activity was compared with the infectious virus titers. Luciferase activity decreased as the concentration of ganciclovir or papaverine increased, while there was a slight decrease in luciferase activity with acyclovir. The level of the decrease in luciferase activity was comparable to the level of decrease in the production of infectious virus. Therefore, the antiviral assay using recombinant virus HCMV/pp28-luc resulted in sensitivity similar to the conventional plaque assay with a significant reduction in assay time.

Expression of Human Cytomegalovirus Immediate Early US3 Gene in Human Fibroblast Cells: Gyu-Cheol Lee , Chong-Kyo Lee , Jin-Hyun Ahn , Chan-Hee Lee; J. Microbiol. 2000;38(1):24-30.

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Abstract PDF: US3 gene is a member of the human cytomegalovirus (HCMV) immediate early gene. Although the precise functions of the US3 gene in HCMV replication and pathogenesis are not known, it has been reported to play a role in inhibiting major histocompatibility class I antigen presentation. For further knowledge of US3 gene expression, rabbit polyclonal antiserum of the US3 gene product was used for indirect immunofluorescence assay. In permissive human foreskin fibroblast (HFF) cells, US3 gene expression was detectable as crescent or half-moon shape in the perinuclear region at immediate early times after virus infection. HFF cells infected with mutant HCMV lacking US3 open reading frames were negative for US3 immunofluorescence assay. Double immunofluorescence assay using monoclonal antibody to gamma adaptin (specific for the Golgi complex) and rabbit anti-US3 antiserum revealed that US3 gene product could be localized to the Golgi complex. At later time after HCMV infection, US3 gene products were detected as globular aggregates in the cytosol. These aggregates were positive for gamma adaptin and stained with preimmune serum, suggesting a nonspecific reaction to the Golgi complex. Northern blot analysis revealed that transcription of US3 was observed only during immediate early times after virus infection (until 6 h postinfection). Therefore US3 gene expression appears to be confined to immediate early time and its gene products are localized to the Golgi complex as cres-cent shaped forms in the perinuclear cytoplasm.

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