Journal of Microbiology

Guiguang Chen

3 Articles

Development of a strategy for the screening of α-glucosidase-producing microorganisms: Bo Zhou+ , Nan Huang+ , Wei Zeng+ , Hao Zhang , Guiguang Chen , Zhiqun Liang; J. Microbiol. 2020;58(2):163-172. Published online January 29, 2020; DOI: https://doi.org/10.1007/s12275-020-9267-4

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α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-D-methylglucoside (AMG) in glass plates was tested for the primary screening of α-glucosidaseproducing strains. First, α-glucosidase-producing Aspergillus niger strains were selected on plates containing EY and AMG based on transparent zone formation resulting from the solubilization of EY by the hydrolyzed product. Conventional
methods
that use trypan blue (TB) and p-nitrophenyl-α-Dglucopyranoside (pPNP) as indicators were then compared with the new strategy. The results showed that EY-containing plates provide the advantages of low price and higher specificity for the screening of α-glucosidase-producing strains. We then evaluated the correlation between the hydrolytic activity of α-glucosidase and diffusion distance, and found that good linearity could be established within a 6–75 U/ml enzyme concentration range. Finally, the hydrolytic and transglycosylation activities of α-glucosidase obtained from the target isolates were determined by EY plate assay and 3,5- dinitrosalicylic acid-Saccharomyces cerevisiae assay, respectively. The results showed that the diameter of the transparent zone varied among isolates was positively correlated with α-glucosidase hydrolytic activity, while good linearity could also be established between α-glucosidase transglycosylation activity and non-fermentable reducing sugars content. With this strategy, 7 Aspergillus niger mutants with high yield of α-glucosidase from 200 obvious single colonies on the primary screen plate were obtained.

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Enhanced Production, Purification, and Characterization of α-Glucosidase from NTG-Mutagenized Aspergillus niger for Industrial Applications
Bowei Yao, Qian Liu, Junjie Xiong, Guangming Feng, Zhongyi Chang, Hongliang Gao
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Molecular Docking and Dynamics Simulations Reveal the Antidiabetic Potential of a Novel Fucoxanthin Derivative from Chnoospora minima
Sachini Sigera, Kavindu D. Theekshana, Sathmi G. Dinanja, Pasindu Eranga, Nayanatharie Karunathilake, Shamali Abeywardhana, Laksiri Weerasinghe, Tharindu Senapathi, Dinithi C. Peiris
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Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
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Development of a PMA‐LAMP visual detection assay for viable Cronobacter sakazakii
Qiming Chen, Yang Yu, Xiaodi Chen, Fangming Tu, Peng Wang, Junyi Huang, Zhanmin Liu
International Journal of Dairy Technology.2024; 77(2): 427. CrossRef
Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation
Chunxu Jiang, Han Wang, Menghan Liu, Li Wang, Ruwen Yang, Peng Wang, Zongmei Lu, Yong Zhou, Zhiming Zheng, Genhai Zhao
Applied Microbiology and Biotechnology.2022; 106(21): 6993. CrossRef
Cloning and characterization of a recombinant α-glucosidase from Ensifer adhaerens NBRC 100388 and evaluation of its glucosyl transfer activity
Tatsuya Suzuki, Miyu Fukaya, Kazuki Takahashi, Michiki Takeuchi, Ryotaro Hara, Jun Ogawa, Makoto Ueda
Biocatalysis and Agricultural Biotechnology.2020; 30: 101837. CrossRef

Analysis of the L-malate biosynthesis pathway involved in poly(β-L-malic acid) production in Aureobasidium melanogenum GXZ-6 by addition of metabolic intermediates and inhibitors: Wei Zeng , Bin Zhang , Qi Liu , Guiguang Chen , Zhiqun Liang; J. Microbiol. 2019;57(4):281-287. Published online February 5, 2019; DOI: https://doi.org/10.1007/s12275-019-8424-0

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Abstract PDF

Poly(β-L-malic acid) (PMA) is a promising polyester formed from L-malate in microbial cells. Malate biosynthesis is crucial for PMA production. Previous studies have shown that the non-oxidative pathway or oxidative pathway (TCA cycle) is the main biosynthetic pathway of malate in most of PMAproducing strains, while the glyoxylate cycle is only a supplementary pathway. In this study, we investigated the effect of exogenous metabolic intermediates and inhibitors of the malate biosynthetic pathway on PMA production by Aureobasidium melanogenum GXZ-6. The results showed that PMA production was stimulated by maleic acid (a fumarase inhibitor) and sodium malonate (a succinate dehydrogenase inhibitor) but inhibited by succinic acid and fumaric acid. This indicated that the TCA cycle might not be the only biosynthetic pathway of malate. In addition, the PMA titer increased by 18.1% upon the addition of glyoxylic acid after 72 h of fermentation, but the PMA titer decreased by 7.5% when itaconic acid (an isocitrate lyase inhibitor) was added, which indicated that malate for PMA production was synthesized significantly via the glyoxylate cycle rather than the TCA cycle. Furthermore, in vitro enzyme activities of the TCA and glyoxylate cycles suggested that the glyoxylate cycle significantly contributed to the PMA production, which is contradictory to what has been reported previously in other PMA-producing A. pullulans.

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Nanomaterials.2021; 11(11): 2996. CrossRef
Biosynthetic Polymalic Acid as a Delivery Nanoplatform for Translational Cancer Medicine
Jianguo Zhang, Deyu Chen, Guoxin Liang, Wenrong Xu, Zhimin Tao
Trends in Biochemical Sciences.2021; 46(3): 213. CrossRef
Polymalate (PMA) biosynthesis and its molecular regulation in Aureobasidium spp.
Cong-Yan Qi, Shu-Lei Jia, Guang-Lei Liu, Lu Chen, Xin Wei, Zhong Hu, Zhen-Ming Chi, Zhe Chi
International Journal of Biological Macromolecules.2021; 174: 512. CrossRef
Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1
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Comparative genome analysis proposes three new Aureobasidium species isolated from grape juice
Cristobal A Onetto, Simon A Schmidt, Michael J Roach, Anthony R Borneman
FEMS Yeast Research.2020;[Epub] CrossRef
Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum
Genan Wang, Bingyi Shi, Pan Zhang, Tingbin Zhao, Haisong Yin, Changsheng Qiao
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A novel PMA synthetase is the key enzyme for polymalate biosynthesis and its gene is regulated by a calcium signaling pathway in Aureobasidium melanogenum ATCC62921
Kai Wang, Zhe Chi, Guang-Lei Liu, Cong-Yan Qi, Hong Jiang, Zhong Hu, Zhen-Ming Chi
International Journal of Biological Macromolecules.2020; 156: 1053. CrossRef
Poly(malic acid) production from liquefied corn starch by simultaneous saccharification and fermentation with a novel isolated Aureobasidium pullulans GXL-1 strain and its techno-economic analysis
Wei Zeng, Bin Zhang, Li Jiang, Yao Liu, Su Ding, Guiguang Chen, Zhiqun Liang
Bioresource Technology.2020; 304: 122990. CrossRef
Recent progress on bio-based production of dicarboxylic acids in yeast
Xi Zhang, Yunying Zhao, Yingli Liu, Jing Wang, Yu Deng
Applied Microbiology and Biotechnology.2020; 104(10): 4259. CrossRef

Identification and characterization of a new agar-degrading strain with the novel properties of saccharides inhibition and nitrogen fixation: Hao Wu , Guiguang Chen , Yaxi Bian , Wei Zeng , Bihong Sun , Zhiqun Liang; J. Microbiol. 2017;55(6):475-482. Published online May 28, 2017; DOI: https://doi.org/10.1007/s12275-017-6464-x

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Abstract PDF

In this study, a new agar-degrading strain was isolated from soil with agar as a sole carbon source and energy. Based on its morphological, physiological, biochemical characterization and 16S rDNA sequence, the strain was identified as Strep-tomyces lavendulae UN-8. The extracellular agarase activity reached 0.03 U/ml after fermentation in shake flask (250 ml), which was close to other reported non-marine micro-organisms. Furthermore, it is interesting that the growth of UN-8 would be inhibited by glucose (40 g/L) and maltose (40 g/L) with the inhibitory rate of 100% and 70%, respec-tively. Besides, UN-8 could be grown on the solid medium without any nitrogen sources, then the possible nitrogen fix-ation gene nifU was cloned from its genomic DNA. The de-duced amino acid sequence of nifU has high similarity (98%) with nitrogen fixation protein NifU from Streptomyces sp. NRRL S-104 (KJY22454.1) and Streptomyces sp. NRRL F-4428 (KJK52526.1) based on NCBI blast. It is suggested that the nifU gene of UN-8 also encoded nitrogen fixation protein NifU. These results provided some new information for the further understanding of agar-degrading strain.

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Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase
Amrit Koirala, Nabilah Ali Alshibli, Bikram K. Das, Volker S. Brözel
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A Novel Strategy to Regulate 1-Deoxynojirimycin Production Based on Its Biosynthetic Pathway in Streptomyces lavendulae
Hao Wu, Ye Guo, Lei Chen, Guiguang Chen, Zhiqun Liang
Frontiers in Microbiology.2019;[Epub] CrossRef

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