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Gil-Jae Joo 5 Articles
Gibberellin-Producing Promicromonospora sp. SE188 Improves Solanum lycopersicum Plant Growth and Influences Endogenous Plant Hormones
Sang-Mo Kang , Abdul Latif Khan , Muhammad Hamayun , Javid Hussain , Gil-Jae Joo , Young-Hyun You , Jong-Guk Kim , In-Jung Lee
J. Microbiol. 2012;50(6):902-909.   Published online December 30, 2012
DOI: https://doi.org/10.1007/s12275-012-2273-4
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AbstractAbstract PDF
Plant growth-promoting rhizobacteria (PGPR) producing gibberellins (GAs) can be beneficial to plant growth and development. In the present study, we isolated and screened a new strain of Promicromonospora sp., SE188, isolated from soil. Promicromonospora sp. SE188 secreted GAs into its growth medium and exhibited phosphate solubilization potential. The PGPR produced physiologically active (GA1 and GA4) and inactive (GA9, GA12, GA19, GA20, GA24, GA34, and GA53) GAs in various quantities detected by GC/MS-SIM. Solanum lycopersicum (tomato) plants inoculated with Promicromonospora sp. SE188 showed a significantly higher shoot length and biomass as compared to controls where PGPR-free nutrient broth (NB) and distilled water (DW) were applied to plants. The presence of Promicromonospora sp. SE188 significantly up-regulated the non C-13 hydroxylation GA biosynthesis pathway (GA12→GA24→GA9→GA4→GA34) in the tomato plants as compared to the NB and DW control plants. Abscisic acid, a plant stress hormone, was significantly down-regulated in the presence of Promicromonospora sp. SE188. Contrarily, salicylic acid was significantly higher in the tomato plant after Promicromonospora sp. SE188 inoculation as compared to the controls. Promicromonospora sp. SE188 showed promising stimulation of tomato plant growth. From the results it appears that Promicromonospora sp. SE188 has potential as a bio-fertilizer and should be more broadly tested in field trials for higher crop production in eco-friendly farming systems.

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Burkholderia sp. KCTC 11096BP as a Newly Isolated Gibberellin Producing Bacterium
Gil-Jae Joo , Sang-Mo Kang , Muhammad Hamayun , Sang-Kuk Kim , Chae-In Na , Dong-Hyun Shin , In-Jung Lee
J. Microbiol. 2009;47(2):167-171.   Published online May 2, 2009
DOI: https://doi.org/10.1007/s12275-008-0273-1
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AbstractAbstract PDF
We isolated 864 bacteria from 553 soil samples and bioassayed them on cucumber and crown daisy for plant growth promotion. A new bacterial strain, Burkholderia sp. KCTC 11096BP gave maximum growth promotion and was selected for further investigations. The culture filtrate of this bacterium was thus analyzed for the presence of gibberellins and we found physiologically active gibberellins were found (GA1, 0.23 ng/100 ml; GA3, 5.11 ng/100 ml and GA4, 2.65 ng/100 ml) along with physiologically inactive GA9, GA12, GA15, GA20, and GA24. The bacterial isolate also solubilised tricalcium phosphate and lowered the pH of the medium during the process. The isolate was identified as a new strain of Burkholderia through phylogenetic analysis of 16S rDNA sequence. Gibberellin production capacity of genus Burkholderia is reported for the first time in current study.

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Gibberellins-Producing Rhizobacteria Increase Endogenous Gibberellins Content and Promote Growth of Red Peppers
Gil-Jae Joo , Young-Mog Kim , Jung-Tae Kim , In-Koo Rhee , Jin-Ho Kim , In-Jung Lee
J. Microbiol. 2005;43(6):510-515.
DOI: https://doi.org/2297 [pii]
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AbstractAbstract PDF
The growth of red pepper plants was enhanced by treatment with the rhizobacterium, Bacillus cereus MJ-1. Red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. This plant growth-promoting rhizobacterium (PGPR) has been reported to produce gibberellins (GAs). Other GAs-producing rhizobacteria, Bacillus macroides CJ-29 and Bacillus pumilus CJ-69, also enhanced the fw of the plants. They were less effective than B. cereus MJ-1, though. The endogenous GAs content of pepper shoots inoculated with MJ-1 was also higher than in shoots inoculated with CJ-29 or CJ-69. When inoculated with MJ-1, bacterial colonization rate of the roots was higher than that of roots inoculated with CJ-29 or CJ-69. These results support the idea that the plant growth-promoting effect of the bacteria also positively related with the efficiency of root colonization by the bacteria. In addition, we identified the major endogenous GAs of the red pepper as originating from both the early C-13 hydroxylation and the early non C-13 hydroxylation pathways, with the latter being the predominant pathway of GA biosynthesis in red pepper shoots.
Molecular Cloning and Expression of a Thermostable Xylose (Glucose) Isomerase Gene, xylA, from Streptomyces chibaensis J-59
Gil-Jae Joo , Jae-Ho Shin , Gun-Young Heo , Young-Mog Kim , In-Koo Rhee
J. Microbiol. 2005;43(1):34-37.
DOI: https://doi.org/2141 [pii]
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AbstractAbstract PDF
In the present study, the xylA gene encoding a thermostable xylose (glucose) isomerase was cloned from Streptomyces chibaensis J-59. The open reading frame of xylA (1167 bp) encoded a protein of 388 amino acids with a calculated molecular mass of about 43 kDa. The XylA showed high sequence homology (92% identity) with that of S. olivochromogenes. The xylose (glucose) isomerase was expressed in Escherichia coli and purified. The purified recombinant XylA had an apparent molecular mass of 45 kDa, which corresponds to the molecular mass calculated from the deduced amino acid and that of the purified wild-type enzyme. The N-terminal sequences (14 amino acid residues) of the purified protein revealed that the sequences were identical to that deduced from the DNA sequence of the xylA gene. The optimum temperature of the purified enzyme was 85oC and the enzyme exhibited a high level of heat stability.
Chlorothalonil-Biotransformation by Glutathione S-Transferase of Escherichia coli
Young-Mog Kim , Kunbawui Park , Soon-Hyun Jung , Jun-Ho Choi , Won-Chan Kim , Gil-Jae Joo , In-Koo Rhee
J. Microbiol. 2004;42(1):42-46.
DOI: https://doi.org/2002 [pii]
  • 622 View
  • 1 Download
AbstractAbstract PDF
It has recently been reported that one of the most important factors of yeast resistance to the fungicide chlorothalonil is the glutathione contents and the catalytic efficiency of glutathione S-transferase (GST) (Shin et al., 2003). GST is known to catalyze the conjugation of glutathione to a wide variety of xenobiotics, resulting in detoxification. In an attempt to elucidate the relation between chlorothalonil detoxification and GST, the GST of Escherichia coli was expressed and purified. The drug hypersensitive E. coli KAM3 cells harboring a plasmid for the overexpression of the GST gene can grow in the presence of chlorothalonil. The purified GST showed chlorothalonil-biotransformation activity in the presence of glutathione. Thus, chlorothalonil is detoxified by the mechanism of glutathione conjugation catalyzed by GST.

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