- Paenibacillus insulae sp. nov., isolated from soil
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Sung-Jun Cho , Sung-Heun Cho , Tae-Su Kim , Suhk-Hwan Park , Seung-Bum Kim , Geon-Hyoung Lee
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J. Microbiol. 2015;53(9):588-591. Published online August 27, 2015
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DOI: https://doi.org/10.1007/s12275-015-4610-x
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 Abstract
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A Gram-stain-positive, motile, endospore-forming, and strictly
aerobic rod-shaped bacterium designated DS80T was isolated
from an island soil. The strain DS80T grew at temperatures
between 15 and 40°C (optimum = 30°C) and at pH values
ranging from 5.0 to 9.0 (optimum = 7.0). The phylogenetic
analysis based on the comparisons of the 16S rRNA gene sequences
showed that the isolate was affiliated to the genus
Paenibacillus and was mostly related to Paenibacillus assamensis
GPTSA11T (with the sequence similarity of 96.33%)
and Paenibacillus urinalis 5402403T(95.48%). The G+C content
of the genomic DNA was 44.0 mol% and the major fatty
acids were anteiso-C15:0, iso-C15:0, iso-C16:0, and C16:1 ω11c.
Strain DS80T contained MK-7 as the major menaquinone,
and phosphatidylglycerol, phosphatidylethanolamine, and
diphosphatidylglycerol as the major polar lipids. The peptidoglycan
contained a major amount of meso-diaminopimelic
acid. The chemotaxonomic profile of strain DS80T was consistent
with that of Paenibacillus. However, the phenotypic
properties clearly separated the strain from other species of
the genus. Accordingly, a new species, Paenibacillus insulae
sp. nov., is proposed (type strain =DS80T =JCM 17278T
=KCTC 13833T).
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Citations
Citations to this article as recorded by  - Validation List no. 227: valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
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- Revegetation of a Lakeside Barren Area by the Application of Plant Growth-promoting Rhizobacteria
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Tae-Seok Ahn , Jong-Ok Ka , Geon-Hyoung Lee , Hong-Gyu Song
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J. Microbiol. 2007;45(2):171-174.
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DOI: https://doi.org/2516 [pii]
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Abstract
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The growth stimulation of wild plants by several bacterial species showing plant growth-promoting capabilities was examined in a barren lakeside area at Lake Paro, Korea. Microbial numbers and activities in the field soil were monitored for 73 days after inoculation of the bacteria. The acridine orange direct counts for the total soil bacterial populations ranged between 2.0-2.3×109 cells/g soil and 1.4-1.8×109 cells/g soil in the inoculated and uninoculated soils, respectively. The numbers of Pseudomonas spp., which is known as a typical plant growth-promoting rhizobacteria, and the total microbial activity were higher in the inoculated soil compared to those in the uninoculated soil. The average shoot and root lengths of the wild plants grown in the inoculated soil were 17.3 cm and 12.4 cm, respectively, and longer than those of 11.4 cm and 8.5 cm in the uninoculated soil. The total dry weight of the harvested wild plants was also higher in the inoculated soil (42.0 g) compared to the uninoculated soil (35.1 g). The plant growth-promoting capabilities of the inoculated bacteria may be used for the rapid revegetation of barren or disturbed land, and as biofertilizer in agriculture.
- Rapid Identification of Vibrio vulnificus in Seawater by Real-Time Quantitative TaqMan PCR
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Hye-Young Wang , Geon-Hyoung Lee
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J. Microbiol. 2003;41(4):320-326.
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Abstract
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In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus.
- Monitoring and Characterization of Bacterial Contamination in a High-Purity Water System Used for Semiconductor Manufacturing
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In Seop Kim , Geon-Hyoung Lee , Kye Joon Lee
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J. Microbiol. 2000;38(2):99-104.
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Abstract
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Hydrogen peroxide has been used in cleaning the piping of an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16 megabyte DRAM semiconductor manufacturing. The level of hydrogen peroxide-resistant bacteria in UHPW water was monitored prior to and after disinfecting the piping with hydrogen peroxide. Most of the bacteria isolated after hydrogen peroxide disinfection were highly resistant to hydrogen peroxide. However, the percentage of resistant bacteria decreased with time. The hydrogen peroxide-resistant bacteria were identified as Micrococcus luteus, Bacillus cereus, Alcaligenes latus, Xanthomonas sp. and Flavobacterium indologenes. The susceptibility of the bacteria to hydrogen peroxide was tested as either planktonic cells or attached cells on glass. Attached bacteria as the biofilm on glass exhibited increased hydrogen peroxide resistance, with the resistance increasing with respect to the age of the biofilm. These results indicate that bacteria resistant to hydrogen peroxide play an important role in biofilm regrowth on piping after hydrogen peroxide treatment. In order to optimize the cleaning strategy for piping of the high-purity water system, the disinfecting effect of hydrogen peroxide and peracetic acid on the bacteria was evaluated. The combined use of hydrogen peroxide and peracetic acid was very effective in killing attached bacteria as well as planktonic bacteria.
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