- Overexpression of Bacterioferritin Comigratory Protein (Bcp) Enhances Viability and Reduced Glutathione Level in the Fission Yeast Under Stress
-
Ga-Young Kang , Eun-Hee Park , Kyunghoon Kim , Chang-Jin Lim
-
J. Microbiol. 2009;47(1):60-67. Published online February 20, 2009
-
DOI: https://doi.org/10.1007/s12275-008-0077-3
-
-
873
View
-
0
Download
-
7
Crossref
-
 Abstract
 PDF
-
The structural gene encoding bacterioferritin comigratory protein (Bcp) was amplified using PCR from the genomic DNA of Schizosaccharomyces pombe, and transferred into the shuttle vector pRS316 to generate the recombinant plasmid pBCP10. The bcp+ mRNA level in the pBCP10-containing yeast cells was significantly higher than that in the control yeast cells, indicating that the cloned gene is functioning. The S. pombe cells harboring the plasmid pBCP10 exhibited higher survival on the solid minimal media with hydrogen peroxide, tert-BOOH or cadmium than the control yeast cells. They also exhibited enhanced cellular viability in the liquid media containing the stressful agents. The increased viabilities of the fission yeast cells harboring the plasmid pBCP10 were also obtained with 0.4% glucose or 0.4% sucrose as a sole carbon source, and nitrogen starvation, compared with those of the control yeast cells. The total glutathione (GSH) content and total GSH/GSSG ratio were significantly higher in the yeast cells harboring the plasmid pBCP10 than in the control yeast cells. In brief, the S. pombe Bcp plays a protective role in the defensive response to oxidative stress possibly via up-regulation of total and reduced glutathione levels.
-
Citations
Citations to this article as recorded by  - Microbially derived glutathione from Eubacterium rectale alleviates oxidative stress and promotes intestinal epithelial recovery
Tanner G. Richie, Hallie Wiechman, Brooke Vogt, Carson Ingold, Leah Heeren, Abigail Kamke, Sophia Pogranichniy, Kourtney Monk, Trey Summers, Qinghong Ran, Soumyadev Sarkar, Brandon L. Plattner, Ashley M. Sidebottom, Eugene B. Chang, Sonny T. M. Lee
Microbiome.2026;[Epub] CrossRef - A unique thioredoxin reductase plays defensive roles against oxidative, nitrosative and nutritional stresses in Schizosaccharomyces pombe
Dam-Jung Ji, Chang-Jin Lim, Kyunghoon Kim
The Korean Journal of Microbiology.2016; 52(1): 1. CrossRef - Thermoresistant properties of bacterioferritin comigratory protein against high temperature stress in Schizosaccharomyces pombe
In Wang Ryu, Su Hee Lee, Hye-Won Lim, Kisup Ahn, Kwanghark Park, Jae-Hoon Sa, Kyung Jin Jeong, Chang-Jin Lim, Kyunghoon Kim
The Korean Journal of Microbiology.2016; 52(4): 398. CrossRef - Role of bacterioferritin comigratory protein and glutathione peroxidase-reductase system in promoting bentazone tolerance in a mutant of Synechococcus elongatus PCC7942
Palash Kumar Das, Suvendra Nath Bagchi
Protoplasma.2012; 249(1): 65. CrossRef - Proteomic profiling of Rhizobium tropiciPRF 81: identification of conserved and specific responses to heat stress
Douglas Fabiano Gomes, Jesiane Stefânia da Silva Batista, Aline Luiza Schiavon, Diva Souza Andrade, Mariangela Hungria
BMC Microbiology.2012;[Epub] CrossRef - Distinct functional roles of peroxiredoxin isozymes and glutathione peroxidase from fission yeast, Schizosaccharomyces pombe
Ji-Sun Kim, Mi-Ae Bang, Song-Mi Lee, Ho-Zoon Chae, Kang-Hwa Kim
BMB Reports .2010; 43(3): 170. CrossRef - Thiol and Sulfenic Acid Oxidation of AhpE, the One-Cysteine Peroxiredoxin from Mycobacterium tuberculosis: Kinetics, Acidity Constants, and Conformational Dynamics
Martín Hugo, Lucía Turell, Bruno Manta, Horacio Botti, Gisele Monteiro, Luis E. S. Netto, Beatriz Alvarez, Rafael Radi, Madia Trujillo
Biochemistry.2009; 48(40): 9416. CrossRef
|
MSK
