- Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber
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Qingqing Yan , Zhouwei Zhang , Yishan Yang , Fusheng Chen , Yanchun Shao
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J. Microbiol. 2018;56(4):255-263. Published online February 28, 2018
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DOI: https://doi.org/10.1007/s12275-018-7425-8
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Monascus spp. are commonly used for a wide variety of applications
in the food and pharmaceutical industries. In previous
studies, the knock-out of mrflbA (a putative regulator
of the G protein α subunit) in M. ruber led to autolysis of
the mycelia, decreased pigmentation and lowered mycotoxin
production. Therefore, we aimed to obtain a comprehensive
overview of the underlying mechanism of mrflbA deletion
at the proteome level. A two-dimensional gel electrophoresis
analysis of mycelial proteins indicated that the abundance
of 178 proteins was altered in the ΔmrflbA strain, 33 of which
were identified with high confidence. The identified proteins
are involved in a range of activities, including carbohydrate
and amino acid metabolism, hyphal development and the oxidative
stress response, protein modification, and the regulation
of cell signaling. Consistent with these findings, the activity
of antioxidative enzymes and chitinase was elevated in
the supernatant of the ΔmrflbA strain. Furthermore, deletion
of mrflbA resulted in the transcriptional reduction of secondary
metabolites (pigment and mycotoxin). In short, the
mutant phenotypes induced by the deletion of mrflbA were
consistent with changes in the expression levels of associated
proteins, providing direct evidence of the regulatory functions
mediated by mrflbA in M. ruber.
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Citations
Citations to this article as recorded by  - Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber
Qianrui Liu, Yunfan Zheng, Baixue Liu, Fufang Tang, Yanchun Shao
Journal of Basic Microbiology.2023; 63(10): 1128. CrossRef - Histone deacetylase MrRpd3 plays a major regulational role in the mycotoxin production of Monascus ruber
Yunfan Zheng, Yueyan Huang, Zejing Mao, Yanchun Shao
Food Control.2022; 132: 108457. CrossRef - Characterization of key upstream asexual developmental regulators in Monascus ruber M7
Lili Jia, Yuyun Huang, Jae-Hyuk Yu, Marc Stadler, Yanchun Shao, Wanping Chen, Fusheng Chen
Food Bioscience.2022; 50: 102153. CrossRef - Quantitative Proteomics Analysis by Sequential Window Acquisition of All Theoretical Mass Spectra–Mass Spectrometry Reveals Inhibition Mechanism of Pigments and Citrinin Production of Monascus Response to High Ammonium Chloride Concentration
Bo Zhou, Yifan Ma, Yuan Tian, Jingbo Li, Haiyan Zhong
Journal of Agricultural and Food Chemistry.2020; 68(3): 808. CrossRef
- Cloning and Functional Analysis of the Gβ Gene Mgb1 and the Gγ Gene Mgg1 in Monascus ruber
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Li Li , Lu He , Yong Lai , Yanchun Shao , Fusheng Chen
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J. Microbiol. 2014;52(1):35-43. Published online January 4, 2014
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DOI: https://doi.org/10.1007/s12275-014-3072-x
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Abstract
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The ascomycetous fungus Monascus ruber is one of the most
well-known species widely used to produce Monascus-fermentation
products for natural food colorants and medicine.
Our previous research on the Gα subunit Mga1 and the regulator
of G protein signaling MrflbA indicated that heterotrimeric
G protein signaling pathways were involved in aspects
of growth, sporulation and secondary metabolite production
in M. ruber. To better understand the G protein signaling
pathways in this fungus, a Gβ subunit gene (Mgb1)
and a Gγ subunit gene (Mgg1) were cloned and investigated
in the current study. The predicted Mgb1 protein consisted
of 353 amino acids and Mgg1 consisted of 94 amino acids,
sharing marked similarity with Aspergillus Gβ and Gγ subunits,
respectively. Targeted deletion (Δ) of Mgb1 or Mgg1
result
ed in phenotypic alterations similar to those resulting
from ΔMga1, i.e., restricted vegetative growth, lowered asexual
sporulation, impaired cleistothecial formation, and enhanced
citrinin and pigment production. Moreover, deletion of Mgg1
suppressed the defects in asexual development and in biosynthesis
of citrinin and pigment caused by the absence of
MrflbA function. These results provide evidence that Mgb1
and Mgg1 form a functional Gβγ dimer and the dimer interacts
with Mga1 to mediate signaling pathways, which are
negatively controlled by MrflbA, for growth, reproduction
and citrinin and pigment biosynthesis in M. ruber.
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Citations
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