Journal of Microbiology

Fen Wang

3 Articles

Erratum: Secretions from Serratia marcescens Inhibit the Growth and Biofilm Formation of Candida spp. and Cryptococcus neoformans: Caiyan Xin , Fen Wang , Jinping Zhang , Quan Zhou , Fangyan Liu , Chunling Zhao , Zhangyong Song; J. Microbiol. 2023;61(4):479-479.; DOI: https://doi.org/10.1007/s12275-023-00037-5

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Erratum: Journal of Microbiology (2023) 61:221–232 https://doi.org/10.1007/s12275-022-00007-3 In this article the acknowledgment has been given erroneously. It should be read as follows: This research was supported financially by the Sichuan Science and Technology Program (2023NSFSC1698, 2023NSFSC0529, 2022NSFSC1539, and 2022YFS0629) and Luzhou (2021-JYJ-73 and 2022-JYJ-159), the Technology Strategic Cooperation Project of Luzhou Municipal People’s Government Southwest Medical University (2020LZXNYDJ38 and 2020LZXNYDJ23), and the Foundation of Southwest Medical University (2021ZKMS008, 2022QN042, 2022QN085, 2022QN102, and 2022QN118). The original article has been corrected.

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Sensitive bioluminescence imaging of cryptococcosis in Galleria mellonella improves antifungal screening under in vivo conditions
Eliane Vanhoffelen, Lori Vermoesen, Lauren Michiels, Katrien Lagrou, Agustin Reséndiz-Sharpe, Greetje Vande Velde
Virulence.2024;[Epub] CrossRef

Secretions from Serratia marcescens Inhibit the Growth and Biofilm Formation of Candida spp. and Cryptococcus neoformans: Caiyan Xin , Fen Wang , Jinping Zhang , Quan Zhou , Fangyan Liu , Chunling Zhao , Zhangyong Song; J. Microbiol. 2023;61(2):221-232. Published online February 21, 2023; DOI: https://doi.org/10.1007/s12275-022-00007-3

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Abstract PDF

Candida spp. and Cryptococcus are conditional pathogenic fungi that commonly infect immunocompromised patients. Over the past few decades, the increase in antifungal resistance has prompted the development of new antifungal agents. In this study, we explored the potential antifungal effects of secretions from Serratia marcescens on Candida spp. and Cryptococcus neoformans. We confirmed that the supernatant of S. marcescens inhibited fungal growth, suppressed hyphal and biofilm formation, and downregulated the expression of hyphae-specific genes and virulence-related genes in Candida spp. and C. neoformans. Furthermore, the S. marcescens supernatant retained biological stability after heat, pH, and protease K treatment. The chemical profile of the S. marcescens supernatant was characterized by ultra-high-performance liquid chromatography–linear ion trap/orbitrap high resolution mass spectrometry analysis and a total of 61 compounds with an mzCloud best match of greater than 70 were identified. In vivo, treatment with the S. marcescens supernatant reduced the mortality of fungi-infected Galleria mellonella. Taken together, our results revealed that the stable antifungal substances in the supernatant of S. marcescens have promising potential applications in the development of new antifungal agents.

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Zuoguiyin modulates M2 macrophage polarization to inhibit glycolysis in esophageal squamous cell carcinoma
Yu Wang, Jianxin Guo, Lu Zhou, Jianfeng Yuan, Ruixue Huang, Tiantian Xing, Liping Chang, Zhongbing Wu, Jing Li, Ming Huang
Journal of Ethnopharmacology.2026; 362: 121404. CrossRef
Bacteria in stable fly (Diptera: Muscidae) feces inform foraging decisions of conspecific flies
Emmanuel Hung, Augustus Negraeff, Caelen Watson, Regine Gries, Pantea Zamani, Aryan Monfared, Cyan Luke, Gerhard Gries, Christopher Geden
Journal of Medical Entomology.2026;[Epub] CrossRef
Antifungal activities of Equol against Candida albicans in vitro and in vivo
Fen Wang, Jinping Zhang, Qian Zhang, Zhangyong Song, Caiyan Xin
Virulence.2024;[Epub] CrossRef
Antifungal Effect of Vitamin D3 against Cryptococcus neoformans Coincides with Reduced Biofilm Formation, Compromised Cell Wall Integrity, and Increased Generation of Reactive Oxygen Species
Jian Huang, Junwen Lei, Anni Ge, Wei Xiao, Caiyan Xin, Zhangyong Song, Jinping Zhang
Journal of Fungi.2023; 9(7): 772. CrossRef

Functional Characterization of the Genes tauO, tauK, and tauI in the Biosynthesis of Tautomycetin: Fen Wang , Rixiang Kong , Bo Liu , Jing Zhao , Rongguo Qiu , Li Tang; J. Microbiol. 2012;50(5):770-776. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2154-x

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Abstract PDF

Tautomycetin is a specific protein phosphatase I inhibitor. In an effort to elucidate the biosynthetic mechanism of tautomycetin, we inactivated genes of the tautomycetin biosynthetic gene cluster, tauI, tauO, and tauK, which encode for putative P450 oxidase, citryl-CoA lyase, and esterase enzymes, respectively. The mutant STQ0606 (ΔtauO) did not produce any detectable amount of tautomycetin intermediates but could convert dialkylmaleic anhydride to tautomycetin, strongly indicating that TauO was involved in dialkylmaleic anhydride biosynthesis. STQ1211 (ΔtauK) accumulated dialkylmaleic anhydride, whereas the cofermentation of STQ1211 (ΔtauK) and STQ0606 (ΔtauO) restored the production of tautomycetin. Together, these results suggest that TauK was responsible for the conjugation of dialkylmaleic anhydride and the polyketide moiety in tautomycetin biosynthesis. The disruption of tauI resulted in the accumulation of 5-des-ketotautomycetin, revealing that TauI was responsible for the oxidation at C5 as the last step. Although the shunt pathways were involved in the biosynthesis of tautomycetin, the main post-polyketide synthase tailoring steps were dehydration, decarboxylation and oxidation, taking place consecutively. This study allowed us to predict the biosynthesis of tautomycetin more accurately and provided novel insights into the mechanism of the biosynthesis of tautomycetin.

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Biocatalytic role of cytochrome P450s to produce antibiotics: A review
Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
Biotechnology and Bioengineering.2023; 120(12): 3465. CrossRef
Biosynthesis, regulation, and engineering of a linear polyketide tautomycetin: a novel immunosuppressant in Streptomyces sp. CK4412
Si-Sun Choi, Hee-Ju Nah, Hye-rim Pyeon, Eung-Soo Kim
Journal of Industrial Microbiology and Biotechnology.2017; 44(4-5): 555. CrossRef
Optimized transformation of Streptomyces sp. ATCC 39366 producing leptomycin by electroporation
Yong-Qiang Fan, Hong-Jian Liu, Li Yan, Yu-Shi Luan, Hai-Meng Zhou, Jun-Mo Yang, Shang-Jun Yin, Yu-Long Wang
Journal of Microbiology.2013; 51(3): 318. CrossRef
A Novel Alkaloid from Marine-Derived Actinomycete Streptomyces xinghaiensis with Broad-Spectrum Antibacterial and Cytotoxic Activities
Wence Jiao, Fenghua Zhang, Xinqing Zhao, Jiehan Hu, Joo-Won Suh, Marie-Joelle Virolle
PLoS ONE.2013; 8(10): e75994. CrossRef

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